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\"RmFicvQBAkgCuC54K47siaoibvbClYbpoQdmoEA7Yo9T0l4l5c9wsK8DWv13zXvBYojNDUX4HpbxOEe8enSicCuVVg\"\/<\/figure><\/div>\n\n<\/div>\n
\u5173\u952e\u8bcd\/\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u6280\u672f \u6587\/\u57fa\u56e0\u6167<\/div>\n
<\/div>\n
2019\u5e741\u67084\u65e5\uff0c\u300aScience\u300b\u520a\u767b\u4e86\u65b0\u5e74\u9996\u7bc7\u5173\u4e8e\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u6280\u672f\u5e94\u7528\u7684\u6587\u7ae0\uff0c\u7814\u7a76\u4eba\u5458\u901a\u8fc7\u5c0f\u578b\u4fbf\u643a\u5f0fOxford Nanopore MinION\u8bbe\u5907\uff0c\u5bf9\u5c3c\u65e5\u5229\u4e9a\u7206\u53d1\u7684\u62c9\u6c99\u70ed\u75ab\u60c5\u8fdb\u884c\u4e86\u52a8\u6001\u5b9e\u65f6\u7684\u57fa\u56e0\u7ec4\u76d1\u6d4b\uff0c\u4ec5\u4e03\u5468\u5c31\u5b8c\u6210\u4e86120\u540d\u60a3\u8005\u6837\u672c\u7684\u5b8f\u57fa\u56e0\u7ec4\u6d4b\u5e8f\uff0c\u63ed\u793a\u4e86\u75c5\u6bd2\u7c7b\u578b\u548c\u4f20\u64ad\u9014\u5f84\u7b49\u65f6\u95f4\u654f\u611f\u7684\u95ee\u9898\u3002\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u6280\u672f\u56e0\u5176\u957f\u8bfb\u957f\u3001\u76f4\u63a5\u6d4b\u5e8f\u3001\u5b9e\u65f6\u3001\u6309\u9700\u6d4b\u5e8f\u548c\u7075\u6d3b\u53ef\u62d3\u5c55\u7b49\u4f18\u52bf\uff08\u8be6\u60c5<\/a>\uff09\uff0c\u5728\u4f17\u591a\u751f\u547d\u79d1\u5b66\u5e94\u7528\u573a\u666f\u4e2d\u5e7f\u6cdb\u6d89\u730e\uff0c\u4ec52018\u5e74\u5c31\u6709\u8d85\u8fc7150\u7bc7\u76f8\u5173\u6587\u7ae0\u53d1\u8868\u3002<\/div>\n
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\"?wx_fmt=jpeg\"\/<\/figure><\/div>\n\n
\u56fe\uff0c2019\u5e741\u67084\u65e5\u520a\u767b\u5728\u300aScience\u300b\u4e0a\u7684\u7814\u7a76<\/div>\n
\u6587\u7ae0\u76ee\u5f55\u5982\u4e0b\uff1a<\/div>\n
\u4e00\u3001\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u6280\u672f\u6700\u65b0\u524d\u6cbf\u5e94\u7528<\/div>\n
1. MinION \u6d4b\u5e8f\u4eea\u7528\u4e8e\u5feb\u901f\u3001\u5b9e\u65f6\u76d1\u6d4b\u75be\u75c5\u7206\u53d1\uff0c\u4e86\u89e3\u75c5\u539f\u4f53\u611f\u67d3<\/div>\n
2. GridION\u548cPromethION\u7528\u4e8e\u5927\u578b\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u548c\u7ec4\u88c5\uff0c\u89e3\u6790\u7ed3\u6784\u53d8\u5f02<\/div>\n
3. \u76f4\u63a5RNA\u6d4b\u5e8f<\/div>\n
\u4e8c\u3001\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u6280\u672f\u6700\u65b0\u6280\u672f\u52a8\u6001<\/div>\n
1. R10 \u7eb3\u7c73\u5b54<\/div>\n
2. Flip-flop \u78b1\u57fa\u8bc6\u522b\u8f6f\u4ef6<\/div>\n
3.\u6d4b\u5e8f\u82af\u7247\u66f4\u65b0\uff1aRev D\u7248\u672c\u00a0\u00a0<\/div>\n
4.\u00a0PromethION x24 \u548c PromethION x48\u8bbe\u5907<\/div>\n
5.\u00a0MinION Mk1c<\/div>\n
\u00a0<\/div>\n
\u4e00\u3001\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u6280\u672f\u6700\u65b0\u524d\u6cbf\u5e94\u7528<\/div>\n
MinION \u6d4b\u5e8f\u4eea\u7528\u4e8e\u5feb\u901f\u3001\u5b9e\u65f6\u76d1\u6d4b\u75be\u75c5\u7206\u53d1\uff0c\u4e86\u89e3\u75c5\u539f\u4f53\u611f\u67d3<\/div>\n
\u5728\u75ab\u60c5\u6d41\u884c\u671f\u95f4\u7ed8\u5236\u75c5\u539f\u4f53\u7684\u8fdb\u5316\u56fe\u8c31\uff0c\u5bf9\u4e86\u89e3\u611f\u67d3\u4f20\u64ad\u6a21\u5f0f\u548c\u5236\u5b9a\u76f8\u5e94\u7684\u63a7\u5236\u65b9\u6848\u81f3\u5173\u91cd\u8981\u3002\u7136\u800c\uff0c\u76f8\u5e94\u7684\u6d4b\u5e8f\u5236\u5e93\u5de5\u4f5c\u91cf\u5927\u4e14\u8017\u8d39\u65f6\u95f4\uff0c\u4ece\u91c7\u6837\u5230\u83b7\u5f97\u7ed3\u679c\u7684\u5468\u671f\u901a\u5e38\u9700\u51e0\u5929\u5230\u4e00\u5468\u751a\u81f3\u66f4\u957f\u3002\u5728\u8fd9\u4e2a\u65f6\u95f4\u654f\u611f\u5e76\u5173\u952e\u7684\u9886\u57df\u4e2d\uff0c\u8f6c\u79fb\u8fd0\u8f93\u6837\u672c\u663e\u5f97\u5e76\u4e0d\u660e\u667a\u3002<\/div>\n\n
\n
\"RmFicvQBAkgB3e5YMGGkhDNw2aW9aLO7vCGrwZm2pAX2NNiboqB2ajoibpp4dSlkh4m2hkbJOOXrgMt9j2or6iagog\"\/<\/figure><\/div>\n\n
\u56fe\uff0cOxford Nanopore\u6d4b\u5e8f\u4eea\u5668<\/div>\n
\u4e0d\u540c\u4e8e\u5176\u4ed6\u6d4b\u5e8f\u4eea\uff0cMinION\u91cd\u91cf\u4e0d\u8db3100g\uff0c\u662f\u4e00\u6b3e\u5b8c\u5168\u4fbf\u643a\u5f0f\u6d4b\u5e8f\u88c5\u7f6e\uff0c\u53ef\u901a\u8fc7\u6807\u51c6\u822a\u7a7a\u516c\u53f8\u8fdb\u884c\u88c5\u8fd0\u3002\u7814\u7a76\u4eba\u5458\u53ef\u5b9e\u5730\u8fdb\u884c\u5206\u6790\uff0c\u672a\u6765\u6709\u671b\u5b9e\u73b0\u5373\u65f6\u68c0\u6d4b\u6216\u8fdc\u7a0b\u68c0\u6d4b\uff08\u76ee\u524d\u4ec5\u4f9b\u7814\u7a76\u4f7f\u7528\uff09\u3002\u8f83\u5927\u578bGridION\u548cPromethION\u8bbe\u5907\u4e5f\u53ef\u7528\u4e8e\u6ee1\u8db3\u66f4\u5927\u6570\u91cf\u7684\u6837\u54c1\u6d4b\u5e8f\u3002\u6240\u6709\u8fd9\u4e9b\u529f\u80fd\u90fd\u4f7f\u6570\u5206\u949f\u5185\u5b8c\u6210\u75c5\u539f\u4f53\u4ece\u91c7\u6837\u5230\u83b7\u5f97\u7ed3\u679c\u7684\u5e38\u89c4\u8bca\u65ad\u8fc7\u7a0b\u9010\u6e10\u6210\u4e3a\u53ef\u80fd\uff0c\u4e14\u6709\u6548\u63a8\u8fdb\u4e86\u4f20\u67d3\u6027\u751f\u7269\u4f53\u7684\u68c0\u6d4b\u548c\u6297\u5fae\u751f\u7269\u836f\u7269\u8010\u836f\u6027\u7814\u7a76\u9886\u57df\u7684\u53d1\u5c55\u3002<\/div>\n
\u00a0<\/div>\n
\u516c\u5171\u536b\u751f\u56e2\u961f\u5df2\u4f7f\u7528\u7eb3\u7c73\u5b54\u6280\u672f\u5bf9\u72c2\u72ac\u75c5\u3001\u62c9\u6c99\u70ed\u3001\u9ec4\u70ed\u75c5\u3001\u6d41\u611f\u3001\u767b\u9769\u70ed\u3001\u759f\u75be\u3001\u5927\u80a0\u6746\u83cc\u3001MRSA\u7b49\u75ab\u60c5\u8fdb\u884c\u4e86\u7814\u7a76\uff0c\u5728\u75ab\u60c5\u7206\u53d1\u521d\u671f\u8fc5\u901f\u5bf9\u75c5\u539f\u4f53\u8fdb\u884c\u5b9e\u65f6\u9274\u5b9a\uff0c\u53ef\u4ee5\u4f7f\u516c\u5171\u536b\u751f\u5b98\u5458\u505a\u51fa\u6070\u5f53\u7684\u54cd\u5e94\u5e76\u5408\u7406\u5206\u914d\u8d44\u6e90\u3002<\/div>\n
2018\u5e748\u6708\uff0c\u4e1c\u82f1\u5409\u5229\u5927\u5b66\u7684Justin O’Grady\u7814\u7a76\u5c0f\u7ec4\u4f7f\u7528\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u6280\u672f\u5feb\u901f\u9274\u5b9a\u51fa\u60a3\u8005\u4e0b\u547c\u5438\u9053\u611f\u67d3\uff08LRTI\uff09\u548c\u8010\u836f\u7279\u5f81\u3002LRTI\u662f\u4e00\u79cd\u81f4\u547d\u4f20\u67d3\u75c5\uff0c\u5feb\u901f\u51c6\u786e\u7684\u68c0\u6d4b\u5bf9\u60a3\u8005\u6cbb\u7597\u548c\u9884\u540e\u8d77\u5230\u5173\u952e\u4f5c\u7528\u3002\u76f8\u8f83\u57f9\u517b\u6cd5\u901a\u5e38\u9700\u8d85\u8fc748\u5c0f\u65f6\u7684\u5178\u578b\u53cd\u5e94\u65f6\u95f4\uff0c\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u53ef\u4ee5\u5c06\u65f6\u95f4\u964d\u81f36\u5c0f\u65f6\u4ee5\u4e0b\uff0c\u6781\u5927\u5730\u7f29\u77ed\u4e86\u7b49\u5f85\u65f6\u95f4\u3002<\/div>\n
\u00a0<\/div>\n
\u5728\u4e16\u754c\u5176\u4ed6\u5730\u65b9\uff0c\u82f1\u56fd\u725b\u6d25\u5927\u5b66\u7814\u7a76\u5c0f\u7ec4\u4f7f\u7528MinION\u5b9e\u73b0\u4e86\u7ed3\u6838\u5206\u679d\u6746\u83cc\u79cd\u5c5e\u7684\u5feb\u901f\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u3002\u901a\u8fc7\u5b9e\u65f6\u7eb3\u7c73\u5b54\u6d4b\u5e8f\uff0c\u7ed3\u6838\u75c5\u81f4\u75c5\u751f\u7269\u4f53\u7684\u6297\u5fae\u751f\u7269\u836f\u7269\u8010\u836f\u6027\u5206\u6790\u6240\u9700\u65f6\u95f4\u4ece8-15\u5468\u7f29\u77ed\u5230\u4ec512\u5c0f\u65f6\u3002\u7f29\u77ed\u65f6\u95f4\u7684\u540c\u65f6\u4f7f\u5f97\u75f0\u6db2\u6837\u672c\u5206\u6790\u66f4\u52a0\u7075\u654f\uff0c\u66f4\u5177\u6210\u672c\u6548\u76ca\u3002<\/div>\n\n
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\"?wx_fmt=jpeg\"\/<\/figure><\/div>\n\n
\u56fe\uff0cMinIT\u793a\u610f\u56fe<\/div>\n
2018\u5e74\uff0cOxford Nanopore\u53d1\u5e03\u4e86\u4e00\u6b3e\u53ef\u4ee5\u642d\u8f7dMinION\/Flongle\u4f7f\u7528\u7684\u5c0f\u578b\u8d85\u7ea7\u8ba1\u7b97\u673a\u2014\u2014MinIT\u4fbf\u643a\u5f0f\u5206\u6790\u4eea\u3002\u5b83\u6bd4\u5178\u578b\u57fa\u4e8eCPU\u7684\u7b14\u8bb0\u672c\u7535\u8111\u5feb7\u500d\uff0c\u5e26\u6709\u9884\u914d\u7f6e\u7684\u63a7\u5236\u548c\u5206\u6790\u8f6f\u4ef6\u8f6f\u4ef6\uff0c\u53ef\u5b9e\u73b0\u5b9e\u65f6\u3001\u9ad8\u901a\u91cf\u7684\u6570\u636e\u5206\u6790\uff0c\u53ef\u4ee5\u5728\u5b9e\u9a8c\u5ba4\u73af\u5883\u4e2d\u6216\u66f4\u591a\u81ea\u7136\u73af\u5883\u4e0b\u4f7f\u7528\u3002\u7814\u7a76\u4eba\u5458\u4f7f\u7528MinION\u6d4b\u5e8f\u4eea\u548cMinIT\u4fbf\u643a\u5f0f\u5206\u6790\u4eea\uff0c\u9762\u4e34\u767d\u4ee4\u6d778\u7ea7\u98ce\u66b4\u7684\u6076\u52a3\u73af\u5883\uff0c\u5728\u963f\u62c9\u65af\u52a0\u6d77\u6d0b\u7814\u7a76\u8239\u4e0a\u5b8c\u6210\u4e86\u5bf9\u6d77\u6d0b\u5fae\u751f\u7269\u7ec4\u7684\u5206\u6790\uff0c\u5728\u6301\u7eed\u6570\u5468\u5230\u6570\u6708\u7684\u6d77\u4e0a\u8003\u5bdf\u671f\u95f4\uff0c\u68c0\u6d4b\u5206\u6790\u5f97\u5230\u7684\u5b9e\u65f6\u6570\u636e\u5bf9\u7406\u89e3\u6d77\u6d0b\u751f\u7269\u591a\u6837\u6027\u5177\u6709\u5f88\u5927\u5e2e\u52a9\u3002\u5728\u5730\u7403\u7684\u53e6\u4e00\u8fb9\uff0c\u4f4d\u4e8e\u4e1c\u975e\u7684\u7814\u7a76\u4eba\u5458\u5728\u5766\u6851\u5c3c\u4e9a\u4e00\u4e2a\u5c0f\u519c\u573a\u7684\u6811\u4e0b\u4f7f\u7528MinIT\u548cMinION\u8fdb\u884c\u5b9e\u9a8c\uff0c\u9274\u5b9a\u4e86\u91cd\u8981\u7684\u7ecf\u6d4e\u4f5c\u7269\u6728\u85af\u643a\u5e26\u7684\u75c5\u6bd2\uff0c\u4ece\u6837\u54c1\u5230\u5f97\u51fa\u7ed3\u679c\u4ec5\u9700\u4e09\u5c0f\u65f6\u3002<\/div>\n
\u00a0<\/div>\n
\u4f7f\u7528GridION\u548cPromethION\u8fdb\u884c\u5927\u578b\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u548c\u7ec4\u88c5\uff0c\u89e3\u6790\u7ed3\u6784\u53d8\u5f02<\/div>\n
\u5927\u591a\u6570\u57fa\u56e0\u7ec4\u542b\u6709\u5927\u91cf\u91cd\u590d\u7684DNA\uff0c\u5982\u8f6c\u5ea7\u5b50\u548c\u57fa\u56e0\u590d\u5236\u7b49\uff0c\u4f20\u7edf\u7684\u77ed\u8bfb\u957f\u53ef\u80fd\u65e0\u6cd5\u8de8\u8d8a\u6bcf\u4e2a\u7ed9\u5b9a\u7684\u91cd\u590d\u533a\u57df\uff0c\u4ea7\u751f\u7684\u57fa\u56e0\u7ec4\u7ec4\u88c5\u53ef\u80fd\u9ad8\u5ea6\u7247\u6bb5\u5316\u3002\u5728\u8fd9\u4e00\u70b9\u4e0a\uff0c\u957f\u8bfb\u957f\u6d4b\u5e8f\u6280\u672f\u5177\u6709\u663e\u8457\u4f18\u52bf\uff0c\u5176\u751f\u6210\u7684\u8bfb\u957f\u66f4\u6709\u53ef\u80fd\u8de8\u8d8a\u6574\u4e2a\u91cd\u590d\u533a\u57df\uff0c\u4ece\u800c\u5c06\u7f3a\u53e3\u6700\u5c0f\u5316\uff0c\u5b9e\u73b0\u57fa\u56e0\u7ec4\u7684\u51c6\u786e\u7ec4\u88c5\u3002\u5982\u540c\u4e00\u4e2a\u62fc\u56fe\uff0c\u62fc\u56fe\u7ec4\u5757\u8d8a\u5927\uff0c\u62fc\u56fe\u8d8a\u5bb9\u6613\u3002\u6d4b\u5e8f\u8bfb\u957f\u8d8a\u957f\uff0c\u5b83\u4e0e\u5176\u4ed6\u8bfb\u957f\u4e4b\u95f4\u7684\u91cd\u53e0\u5c31\u8d8a\u591a\u3002\u56e0\u6b64\uff0c\u5c06\u8d8a\u5bb9\u6613\u5c06DNA\u7247\u6bb5\u7ec4\u88c5\u56de\u6b63\u786e\u7684\u987a\u5e8f\u3002\u4e0e\u5176\u5b83\u6280\u672f\u4e0d\u540c\uff0c\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u7684\u8bfb\u957f\u7247\u6bb5\u4e0d\u53d7\u6d4b\u5e8f\u4eea\u7684\u9650\u5236\uff0c2018\u5e745\u6708\uff0c\u82f1\u56fd\u8bfa\u4e01\u6c49\u5927\u5b66\u7684\u79d1\u5b66\u5bb6Matt Loose\u6253\u7834\u4e86\u6b64\u524d1Mb\u7684\u8bfb\u957f\u8bb0\u5f55\uff0c\u83b7\u5f97\u8fc4\u4eca\u4e3a\u6b62\u6700\u957f\u7684\u88ab\u8fde\u7eed\u6d4b\u5e8f\u7684DNA\u7247\u6bb5\uff0c\u957f\u5ea6\u7ea6230\u4e07\u4e2a\u78b1\u57fa\u3002<\/div>\n
\u00a0<\/div>\n
2018\u5e741\u6708\uff0c\u300aNature Biotechnology\u300b\u6742\u5fd7\u53d1\u6587\uff0c\u7814\u7a76\u4eba\u5458\u4f7f\u7528\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u6280\u672f\u5b8c\u6210\u4e86\u8d85\u957f\u8bfb\u957f\u7684\u4eba\u7c7b\u57fa\u56e0\u7ec4\u7ec4\u88c5\uff0c\u5728\u63d0\u9ad8\u7ec4\u88c5\u8fde\u7eed\u6027\u7684\u540c\u65f6\uff0c\u8fd8\u80fd\u89e3\u6790\u5927\u578b\u7684\u7ed3\u6784\u590d\u6742\u533a\u57df\uff0c\u8fd9\u4e5f\u662f\u8fc4\u4eca\u4e3a\u6b62\u4f7f\u7528\u5355\u4e00\u6280\u672f\u5b8c\u6210\u7684\u6700\u5b8c\u6574\u7684\u4eba\u7c7b\u57fa\u56e0\u7ec4\u7ec4\u88c5\u30022017\u5e74\u7b2c\u4e00\u4e2a\u7eb3\u7c73\u5b54\u4eba\u7c7b\u57fa\u56e0\u7ec4\u53d1\u8868\u65f6\uff0c\u7814\u7a76\u8005\u4f7f\u7528\u4e86MinION\u6d4b\u5e8f\u4eea\u7684\u8bb8\u591a\u6d4b\u5e8f\u82af\u7247\u6765\u5b8c\u6210\u3002\u800c\u5982\u4eca\uff0c\u968f\u7740\u6280\u672f\u7684\u4e0d\u65ad\u53d1\u5c55\u548c\u63d0\u5347\uff0c\u76f8\u540c\u768491.2Gb\u6570\u636e\u53ea\u9700\u51e0\u5f20\u4fbf\u643a\u6d4b\u5e8f\u4eeaMinION\u82af\u7247\uff0c\u751a\u81f3\u53ea\u8981\u4e00\u5f20\u9ad8\u901a\u91cf\u6d4b\u5e8f\u4eeaPromethION\u82af\u7247\u5c31\u80fd\u83b7\u5f97\u3002<\/div>\n

2018\u5e743\u6708\u300aNature Biotechnology\u300b\u6742\u5fd7\u53d1\u8868\u52a0\u5dde\u5927\u5b66\u5723\u514b\u9c81\u5179\u5206\u6821Karen Miga\u535a\u58eb\u9886\u5bfc\u7684\u4e00\u9879\u6709\u5173Y\u67d3\u8272\u4f53\u7740\u4e1d\u7c92\u7684\u7814\u7a76\uff0c\u7531\u4e8e\u4eba\u7c7b\u7740\u4e1d\u7c92\u7531\u5927\u91cf\u8fd1\u4f3c\u76f8\u540c\u7684\u4e32\u8054\u91cd\u590d\u7ec4\u6210\uff0c\u96be\u4ee5\u4f7f\u7528\u77ed\u8bfb\u5e8f\u5217\u6280\u672f\u8fdb\u884c\u7ec4\u88c5\uff0c\u8be5\u7814\u7a76\u5229\u7528\u7eb3\u7c73\u5b54\u6280\u672f\u4ea7\u751f\u7684\u957f\u8bfb\u957f\uff0c\u5c55\u793a\u4e86\u4f7f\u7528\u7eb3\u7c73\u5b54\u6280\u672f\u4ea4\u4ed8\u7684\u9996\u4e2a\u5b8c\u6574\u548c\u51c6\u786e\u7684\u4eba\u7c7b\u7740\u4e1d\u7c92\u5e8f\u5217\u30022019\u5e741\u6708\u521d\u300aNature Communication\u300b\u7684\u4e00\u7bc7\u6587\u7ae0\u5c55\u793a\u4e86\u9ad8\u5ea6\u8fde\u7eed\u548c\u5b8c\u6574\u7684\u975e\u6d32\u8d77\u6e90\u7684\u9996\u4e2a\u4eba\u7c7bY\u67d3\u8272\u4f53\u7ec4\u88c5\uff0c\u5e76\u5c06\u7ec4\u88c5\u7684\u8fde\u7eed\u6027\u8f83\u4e4b\u524d\u7684\u65b9\u6cd5\u63d0\u9ad8\u4e86800\uff05\u4ee5\u4e0a\u3002\u00a0<\/p>\n

2017\u5e7412\u6708\u4e2d\u56fd\u79d1\u5b66\u5bb6\u5ba3\u5e03\u7387\u5148\u5b8c\u6210\u83ca\u82b1\u7684\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\uff0c\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u88ab\u7528\u4e8e\u8fdb\u884c\u4ece\u5934\u6d4b\u5e8f\u548c\u7ec4\u88c5C.nankingense\u57fa\u56e0\u7ec4\u3002\u83ca\u82b1\u57fa\u56e0\u7ec4\u9ad8\u5ea6\u590d\u6742\uff0c\u5927\u5c0f\u7ea63.07 Gb\uff0c\u5177\u6709\u9ad8\u6742\u5408\u6027\u3001\u9ad8\u5ea6\u76f8\u4f3c\u7684\u957f\u91cd\u590d\u5e8f\u5217\uff0c\u8bc1\u660e\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u6280\u672f\u80fd\u591f\u63d0\u4f9b\u5927\u91cf\u7684\u8bfb\u957f\u53ca\u76f4\u63a5\u6d4b\u5e8f\u6570\u636e\u3002<\/div>\n
\u00a0<\/div>\n
Oxford Nanopore\u4e8e2018\u5e74\u53d1\u5e03\u4e86\u9ad8\u901a\u91cf\u6d4b\u5e8f\u8bbe\u5907PromethION\uff0c\u53ef\u7528\u4e8e\u9488\u5bf9\u5927\u578b\u57fa\u56e0\u7ec4\u548c\u5927\u578b\u6d4b\u5e8f\u9879\u76ee\u7684\u957f\u8bfb\u957f\u3001\u76f4\u63a5\u6d4b\u5e8f\u3002\u968f\u7740\u6d4b\u5e8f\u6027\u80fd\u4e0d\u65ad\u63d0\u5347\uff0c\u76ee\u524d\u5df2\u6709\u79d1\u5b66\u5bb6\u5728\u5355\u5f20\u6d4b\u5e8f\u82af\u7247\u83b7\u5f97148Gb\u7684\u6d4b\u5e8f\u6570\u636e\uff0c \u5185\u90e8\u6d4b\u8bd5\u4e2d\u5df2\u83b7\u5f97220Gb\u3002\u57282018\u5e74\u7f8e\u56fd\u4eba\u7c7b\u9057\u4f20\u5b66\u5927\u4f1a\u4e0a\uff0c\u6765\u81eaVIB\u7684\u56e2\u961f\u53d1\u5e03\u4e86\u9996\u4e2a\u4f7f\u7528PromethION\u8fdb\u884c\u7684\u4e00\u4e2a\u4eba\u7c7b\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u6587\u7ae0\uff0c\u6807\u5fd7\u7740\u5927\u578b\u3001\u53ef\u8d1f\u62c5\u8d77\u7684\u957f\u8bfb\u957f\u6d4b\u5e8f\u5df2\u7ecf\u5230\u6765\u3002PromethION\u6b63\u5728\u88ab\u8d8a\u6765\u8d8a\u591a\u7684\u7528\u4e8e\u9ad8\u5f71\u54cd\u529b\u7684\u9879\u76ee\u4e2d\uff0c\u5305\u62ec\u79cd\u7fa4\u89c4\u6a21\u7684\u57fa\u56e0\u7ec4\u7814\u7a76\uff0c\u4ee5\u53ca\u690d\u7269\u548c\u4eba\u7c7b\u57fa\u56e0\u7ec4\u3002<\/div>\n
\u00a0<\/div>\n
\u76f4\u63a5RNA\u6d4b\u5e8f<\/div>\n
2018\u5e74\u521d\uff0c\u9996\u4e2aRNA\u76f4\u63a5\u6d4b\u5e8f\u7684\u6587\u7ae0\u8363\u767b\u300aNature Methods\u300b\u671f\u520a\u5c01\u9762\uff0c\u63cf\u8ff0\u4e86\u65e0\u9700\u8fdb\u884c\u9006\u8f6c\u5f55\u6216\u6269\u589e\u6b65\u9aa4\uff0c\u5141\u8bb8\u751f\u6210\u5168\u957f\u3001\u94fe\u7279\u5f02\u6027\u7684RNA\u5e8f\u5217\uff0c\u4fee\u9970\u4fe1\u606f\u88ab\u5b8c\u6574\u4fdd\u7559\u7684RNA\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u6280\u672f\u30022018\u5e744\u6708\uff0c\u7f8e\u56fd\u75be\u75c5\u63a7\u5236\u548c\u9884\u9632\u4e2d\u5fc3\uff08CDC\uff09\u7684\u5fae\u751f\u7269\u5b66\u5bb6John Barnes\u7387\u9886\u7814\u7a76\u56e2\u961f\uff0c\u9996\u6b21\u4ee5\u5176\u5929\u7136\u5f62\u5f0f\u6d4b\u5e8f\u4e86\u5b8c\u6574\u6d41\u611f\u75c5\u6bd2RNA\u57fa\u56e0\u7ec4\u3002\u5728\u300aNature\u300b\u6742\u5fd7\u53d1\u8868\u7684\u65b0\u95fb\u8bc4\u8bba\u6587\u7ae0\u4e2d\uff0cJohn Barnes\u8868\u793a\uff1a\u201c\u8fd9\u662f\u7b2c\u4e00\u6b21\uff0c\u6211\u4eec\u771f\u6b63\u5f00\u59cb\u89c2\u5bdf\u57fa\u56e0\u7ec4\u5728\u539f\u59cb\u72b6\u6001\u4e0b\u7684\u672c\u8d28\uff0c\u5f00\u8f9f\u4e86\u975e\u5e38\u591a\u7684\u53ef\u80fd\u3002\u201d<\/div>\n\n
\n
\"RmFicvQBAkgB3e5YMGGkhDNw2aW9aLO7vewwLOyicntGQSC6cCS01LeyUuiacicGk0k5fGxJTHEJ1xndqfp6tpia7ibw\"\/<\/figure><\/div>\n\n
\u56fe\uff0cMinION\u5df2\u7ecf\u5728\u56fd\u9645\u7a7a\u95f4\u7ad9\u4e0a\u6267\u884c\u4e86\u7eb3\u7c73\u5b54DNA\u548c\u76f4\u63a5RNA\u6d4b\u5e8f\u3002\u56fe\u7247\u6765\u6e90\uff1aOxford Nanopore \u6280\u672f\u539f\u7406\u624b\u518c<\/div>\n
2018\u5e749\u6708\uff0c\u7f8e\u56fdNASA\u822a\u5929\u5c40\u5b87\u822a\u5458\u4f7f\u7528MinION\u9996\u6b21\u5728\u5b87\u5b99\u56fd\u9645\u7a7a\u95f4\u7ad9\u5b8c\u6210\u4e86\u5bf9RNA\u7684\u76f4\u63a5\u6d4b\u5e8f\uff0c\u6709\u52a9\u4e86\u89e3\u4ece\u5fae\u751f\u7269\u5230\u690d\u7269\u518d\u5230\u5b87\u822a\u5458\u7b49\u5404\u79cd\u751f\u7269\u5982\u4f55\u9002\u5e94\u56fd\u9645\u7a7a\u95f4\u7ad9\u7684\u751f\u6d3b\u6761\u4ef6\uff0c\u6539\u5584\u5b87\u822a\u5458\u5065\u5eb7\u72b6\u6001\u3002\u968f\u540e11\u6708\u9884\u5370\u5728\u300aBioRxiv\u300b\u7684\u4e00\u7bc7\u91cc\u7a0b\u7891\u5f0f\u7684\u6587\u7ae0\u4e2d\uff0c\u7814\u7a76\u8005\u4eec\u4f7f\u7528\u7eb3\u7c73\u5b54\u5929\u7136RNA\u6d4b\u5e8f\u8fdb\u884c\u4e86\u4eba\u7c7b\u591a\u805a\u817a\u82f7\u9178\u5c3e\uff08poly-A\uff09\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\u3002<\/div>\n
RNA\u6d4b\u5e8f\u662f\u7814\u7a76\u4eba\u7c7b\u9057\u4f20\u5b66\u548c\u75be\u75c5\u76f8\u5173\u75c5\u7406\u5b66\u7684\u5b9d\u8d35\u5de5\u5177\uff0c\u8fd1\u5e74\u5728\u751f\u7269\u5b66\u3001\u533b\u5b66\u4e43\u81f3\u519c\u4e1a\u73af\u5883\u4e2d\u7684\u5404\u4e2a\u9886\u57df\u90fd\u53d6\u5f97\u4e86\u524d\u6240\u672a\u6709\u7684\u53d1\u5c55\u3002\u7531\u4e8e\u4f20\u7edf\u6d4b\u5e8f\u65b9\u6cd5\u8fdb\u884c\u7684RNA\u6d4b\u5e8f\u5206\u6790\u9700\u5c06RNA\u8f6c\u5316\u4e3a\u4e92\u8865DNA\uff08cDNA\uff09\u94fe\uff0c\u800c\u5e76\u975e\u6240\u6709\u7684\u8f6c\u5f55\u7269\u90fd\u80fd\u4ee5\u76f8\u540c\u7684\u6548\u7387\u6269\u589e\uff0c\u8fd9\u4f1a\u5bfc\u81f4\u4e00\u4e9b\u79cd\u7c7bRNA\u6269\u589e\u4e2d\u65ad\uff0c\u4ee5\u53ca\u5176\u5b83\u79cd\u7c7bRNA\u7684\u8fc7\u5ea6\u6269\u589e\uff0c\u4ece\u800c\u5f15\u5165\u9006\u8f6c\u5f55\u6216\u6269\u589e\u5e26\u6765\u7684\u504f\u597d\u6027\u3002\u8fd9\u4e9b\u65b9\u6cd5\u7684\u5c40\u9650\u6027\u4f53\u73b0\u5728\u83b7\u5f97\u5168\u957f\u8f6c\u5f55\u7269\u3001\u95ee\u9898\u5f02\u6784\u4f53\u7684\u8bc6\u522b\u548c\u91cf\u5316\u3001\u6587\u5e93\u6784\u5efa\u76f8\u5173\u7684\u504f\u501a\uff08PCR\u3001\u9006\u8f6c\u5f55\u7b49\uff09\u7b49\u65b9\u9762\u3002<\/div>\n
\u00a0<\/div>\n
\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u80fd\u63d0\u4f9b\u957f\u8bfb\u957f\u5728\u8f6c\u5f55\u7ec4\u5206\u6790\u4e2d\u6240\u5360\u6709\u7684\u6240\u6709\u4f18\u52bf\uff0c\u751f\u6210\u5168\u957f\u8f6c\u5f55\u672c\uff0c\u9002\u7528\u4e8e\u5f02\u6784\u4f53\u4ee5\u53ca\u4e30\u5ea6\u7684\u7cbe\u786e\u9274\u5b9a\u3002\u4fbf\u643a\u3001\u5feb\u901f\u7684\u5b9e\u9a8c\u5ba4\u5236\u5907\u6b65\u9aa4\u548c\u5b9e\u65f6\u5206\u6790\u6d41\u7a0b\uff0c\u4f7f\u5f97\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u5728\u75c5\u6bd2\u6027\u75c5\u539f\u4f53\u7814\u7a76\u9886\u57df\uff0c\u7279\u522b\u662f\u5b9e\u5730\u4f5c\u4e1a\u73af\u5883\u4e2d\u5177\u6709\u4f18\u52bf\u3002\u65e0\u9006\u8f6c\u5f55\u548cPCR\u6269\u589e\u504f\u501a\u7684RNA\u76f4\u63a5\u6d4b\u5e8f\u7684\u51fa\u73b0\u66f4\u662f\u8fdb\u4e00\u6b65\u63a8\u52a8\u4e86\u8f6c\u5f55\u7ec4\u5b66\u7684\u5206\u6790\u3002RNA\u76f4\u63a5\u6d4b\u5e8f\u8fd8\u53ef\u4ee5\u901a\u8fc7\u5355\u4e2a\u6587\u5e93\u5236\u5907\u9274\u5b9a\u591a\u79cdRNA\u4fee\u9970\uff0c\u65e0\u9700\u7ecf\u8fc7\u5197\u957f\u7684\u4e9a\u786b\u9178\u6c22\u76d0\u8f6c\u5316\u6216\u6297\u4f53\u6c89\u6dc0\u64cd\u4f5c\u3002\u8fd9\u4e9b\u7279\u70b9\uff0c\u518d\u7ed3\u5408GridION X5\u548cPromethION\u5e73\u53f0\u6240\u63d0\u4f9b\u7684\u89c4\u6a21\u4e0a\u7684\u53ef\u6269\u5c55\u6027\uff0c\u501f\u7528\u5370\u7b2c\u5b89\u7eb3\u5927\u5b66\u4f2f\u660e\u987f\u5206\u6821Marinov\u535a\u58eb\u7684\u8bdd\uff0c\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u6210\u4e3a\u4e86 \u201c\u8f6c\u5f55\u7ec4\u7814\u7a76\u4e2d\u6700\u4ee4\u4eba\u5174\u594b\u7684\u53d1\u5c55\u201d\u3002<\/div>\n
\u00a0<\/div>\n
\u4e8c\u3001\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u6280\u672f\u6700\u65b0\u6280\u672f\u52a8\u6001<\/div>\n
R10 \u7eb3\u7c73\u5b54<\/div>\n
R10 \u662f\u4e00\u4e2a\u65b0\u7eb3\u7c73\u5b54\u8bbe\u8ba1\uff0c\u9884\u671f\u57282019\u5e74\u65e9\u4e9b\u65f6\u5019\u53d1\u5e03\u3002R10\u7248\u7eb3\u7c73\u5b54\u7684\u201c\u6876\u8eab\u201d\u66f4\u957f\uff0c\u5177\u6709\u4e24\u5bf9Reader heads\u3002 \u66f4\u591a\u7684\u78b1\u57fa\u4e3b\u5bb0\u4fe1\u53f7\u4ee5\u8fbe\u5230\u66f4\u9ad8\u7684\u51c6\u786e\u5ea6\uff0c\u5c24\u5176\u662f\u5728\u5747\u805a\u7269\uff08\u7531\u4e00\u79cd\u5355\u4f53\u805a\u5408\u6210\u7684\uff09\u65b9\u9762\u3002\u78b1\u57fa\u8d28\u91cf\u503c\u662f\u8861\u91cf\u6d4b\u5e8f\u8d28\u91cf\u7684\u91cd\u8981\u6307\u6807\uff0c\u8d28\u91cf\u503c\uff08Q\uff09\u8d8a\u9ad8\u4ee3\u8868\u78b1\u57fa\u88ab\u6d4b\u9519\u7684\u6982\u7387\u8d8a\u5c0f\u3002\u5728\u5185\u90e8\u6d4b\u8bd5\u4e2d\uff0c\u4ee575x\u8986\u76d6\u5ea6\uff0c\u65b0\u7684R10\u7eb3\u7c73\u5b54\u80fd\u591f\u63d0\u4f9b\u8d28\u91cf\u503c>Q40\u4ee5\u53ca\u4e00\u81f4\u6027\uff08identity\uff09\u8d28\u91cf\u503cQ50 \u3002<\/div>\n\n
\n
\"?wx_fmt=jpeg\"\/<\/figure><\/div>\n\n
\u56fe\uff0c\u65b0R10\u7eb3\u7c73\u5b54<\/div>\n
Flip-flop \u78b1\u57fa\u8bc6\u522b\u8f6f\u4ef6<\/div>\n\n
\n
\"0?wx_fmt=jpeg\"\/<\/figure><\/div>\n\n
\u56fe\uff0c\u57fa\u4e8e\u201cFlip-flop\u201d\u7b97\u6cd5\u7684\u65b0\u78b1\u57fa\u8bc6\u522b\u8f6f\u4ef6<\/div>\n
\u65b0\u7684\u78b1\u57fa\u8bc6\u522b\u8f6f\u4ef6\u201cFlip-flop\u201d\u4e3aR9\u7684\u5171\u6709\u5e8f\u5217\u4e00\u81f4\u6027\u63d0\u4f9b\u4e86\u5927\u7684\u63d0\u5347\u2014\u2014\u91cd\u65b0\u8bc6\u522b\u73b0\u6709\u6570\u636e\u53ef\u53d6\u5f97Q37\u3002\u8be5\u8f6f\u4ef6\u57282018\u5e7412\u6708\u4e2d\u65ec\u901a\u8fc7Guppy\u8f6f\u4ef6\u53d1\u5e03\u3002Guppy\u662fOxford Nanopore\u63d0\u4f9b\u7684\u4e00\u6b3e\u751f\u6210\u78b1\u57fa\u8bc6\u522b\u8f6f\u4ef6\uff0c\u7ecf\u8fc7\u4f18\u5316\uff0c\u53ef\u4e0e\u78b1\u57fa\u8bc6\u522b\u52a0\u901f\u5668\u4e00\u8d77\u8fd0\u884c\u3002<\/div>\n
\u00a0<\/div>\n
\u6d4b\u5e8f\u82af\u7247\u66f4\u65b0\uff1aRev D\u7248\u672c<\/div>\n
2018\u5e7410\u6708\uff0cOxford Nanopore\u53d1\u5e03\u4e86\u5168\u65b0\u7248\u201cRev D\u201dASIC\u7684MinION\u548cGridION\u6d4b\u5e8f\u82af\u7247\u3002\u65b0\u7248\u672c\u5ef6\u957f\u4e86\u6d4b\u5e8f\u82af\u7247\u53ef\u7528\u4e8eDNA\u6d4b\u5e8f\u6216RNA\u6d4b\u5e8f\u7684\u65f6\u95f4\uff0c\u5c06DNA\u5e8f\u5217\u6570\u636e\u7684\u603b\u4ea7\u91cf\u63d0\u9ad8\u5230\u6bcf\u4e2a\u6d4b\u5e8f\u82af\u7247\u53ef\u9ad8\u81f330 Gb\u3002\u5728\u6b64\u6027\u80fd\u4e0b\uff0c\u76f8\u5f53\u4e8e500\u7f8e\u5143\u5373\u53ef\u6d4b\u5e8f\u8986\u76d6\u5ea6\u4e3a10x\u7684\u4eba\u7c7b\u57fa\u56e0\u7ec4\u3002<\/div>\n
\u00a0<\/div>\n
\u81ea\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u6280\u672f\u63a8\u51fa\u4ee5\u6765\uff0c\u5355\u4e2a\u6d4b\u5e8f\u82af\u7247\u53ef\u4ea7\u751f\u7684\u6d4b\u5e8f\u6570\u636e\u91cf\u4e0d\u65ad\u5267\u589e\u3002\u6b64\u524d\u7684\u4ea7\u91cf\u589e\u957f\u53d7\u5230\u4e86\u5404\u79cd\u6280\u672f\u8981\u7d20\u7684\u63a8\u52a8\uff0c\u5305\u62ec\u63d0\u9ad8DNA\u7247\u6bb5\u901a\u8fc7\u7eb3\u7c73\u5b54\u7684\u901f\u7387\uff0c\u4ece\u63a8\u51fa\u65f6\u7684\u6bcf\u79d235\u4e2a\u78b1\u57fa\u63d0\u5347\u5230\u73b0\u5728\u6bcf\u79d2450\u4e2a\u78b1\u57fa\u3002\u6b64\u5916\u8fd8\u5305\u62ec\u53d1\u5e03\u4e86\u6d4b\u5e8f\u8bbe\u5907\u63a7\u5236\u8f6f\u4ef6MinKNOW\u7684\u66f4\u65b0\u7248\u672c\uff1a\u7528\u6237\u754c\u9762\u66f4\u53cb\u597d\uff1b\u00a0\u65b0\u529f\u80fd\u652f\u6301\u66f4\u9ad8\u6570\u636e\u4ea7\u51fa\uff1b\u201c\u7535\u538b\u63a7\u5236\u201d\u66f4\u597d\u5730\u9002\u5e94\u5b9e\u9a8c\uff0c\u4fdd\u6301\u4fe1\u53f7\u7684\u4e00\u81f4\u6027\uff1b\u66f4\u65b0\u6838\u5fc3API\uff08\u5e94\u7528\u7a0b\u5e8f\u7f16\u7a0b\u63a5\u53e3\uff09\uff1b\u4efb\u52a1\u65f6\u95f4\u8868\u4f7f\u7528\u6237\u80fd\u591f\u8fc5\u901f\u68c0\u67e5\u6587\u5e93\u8d28\u91cf\u548c\u6c61\u67d3\u60c5\u51b5\uff0c\u6839\u636e\u559c\u597d\u51b3\u5b9a\u662f\u5426\u505c\u6b62\u8fd0\u884c\u3001\u6e05\u6d17\u82af\u7247\u3001\u518d\u6b21\u8fd0\u884c\u3002\u5e76\u53d1\u5e03\u65b0\u7248\u8bd5\u5242\u76d2LSK109\uff0c\u53ef\u5728\u5355\u4e2a\u8bd5\u5242\u76d2\u4e2d\u5b9e\u73b0\u8bfb\u957f\u957f\u5ea6\u548c\u4ea7\u91cf\u6700\u5927\u5316\u3002<\/div>\n
\u00a0<\/div>\n
PromethION x24 \u548c PromethION x48\u8bbe\u5907<\/div>\n
\u57282019\u5e74\u5c06\u53d1\u5e03\u4e24\u4e2a\u65b0\u7248\u7684PromethION\u8bbe\u5907\uff1a24\u5f20\u6d4b\u5e8f\u82af\u7247\u7684 x24\u7248, \u4ee5\u53ca48\u5f20\u6d4b\u5e8f\u82af\u7247\u7684x48\u7248\u3002\u5f53\u524d\u6700\u4f73\u7684PromethION\u7528\u6237\u8fd0\u884c\u5df2\u7ecf\u975e\u5e38\u63a5\u8fd1150Gb \u6bcf\u5f20\u82af\u7247\uff0c\u4ea7\u91cf\u4e2d\u4f4d\u6570\u4e3a~60Gb\u3002\u76ee\u524d\u7684\u5185\u90e8\u8bb0\u5f55\u4e3a220Gb\u6bcf\u5f20\u82af\u7247\uff0c\u8fd0\u884c\u5168\u90e824\u5f20\u82af\u7247\u4e3a3.8 Tb\u3002\u610f\u5473\u7740\u4e00\u53f0\u53ef\u542f\u752848\u5f20\u82af\u7247\u7684\u8bbe\u5907\u5e94\u80fd\u591f\u4ea4\u4ed87Tb\u6570\u636e\u3002<\/div>\n\n
\n
\"?wx_fmt=jpeg\"\/<\/figure><\/div>\n\n
\u56fe\uff0c\u00a0\u65b0\u7248PromethION x24 \u548c PromethION x48<\/div>\n
MinION Mk1c<\/div>\n
MinION Mk1c \u662f\u4e00\u4e2a\u96c6MinION\u6d4b\u5e8f\u4eea\u3001\u4fbf\u643a\u5f0f\u5206\u6790\u4eeaMinIT \u548c\u4e00\u4e2a\u5c4f\u5e55\u4e3a\u4e00\u4f53\u7684\u8bbe\u5907\uff0c\u53ef\u901a\u8fc7WiFi\/\u84dd\u7259\u548cSIM\u5361\u8fde\u7f51\u3002MinION Mk1c \u6b63\u5728\u8fdb\u4e00\u6b65\u7684\u7814\u53d1\u4e2d\u3002<\/div>\n\n
\n
\"?wx_fmt=jpeg\"\/<\/figure><\/div>\n\n
\u56fe\uff0cMinION Mk1c<\/div>\n
\u53c2\u8003\u94fe\u63a5\uff1a<\/div>\n
1. https:\/\/www.nature.com\/articles\/nbt.4109<\/div>\n
2. https:\/\/www.nature.com\/articles\/s41467-018-07885-5<\/div>\n
3. https:\/\/www.nature.com\/articles\/nbt.4060<\/div>\n
4. https:\/\/www.cell.com\/molecular-plant\/pdf\/S1674-2052(18)30308-3.pdf<\/div>\n
5. https:\/\/erj.ersjournals.com\/content\/52\/suppl_62\/PA5308<\/div>\n
6. https:\/\/nanoporetech.com\/about-us\/news\/longer-and-longer-dna-sequence-more-two-million-bases-now-achieved-nanopore<\/div>\n
7. https:\/\/www.biorxiv.org\/content\/early\/2018\/11\/09\/459529<\/div>\n
8. https:\/\/nanoporetech.com\/about-us\/news\/oxford-nanopore-releases-rev-d-flow-cells-enabling-increase-data-yields<\/div>\n
\u00a0\u7f16\u8005\uff1aCandice\u00a0 \u6821\u5bf9\uff1aEric\u00a0 \u5ba1\u6838\uff1aMark<\/div>\n\n
\n
\"RmFicvQBAkgCcBof1z1ClbNQiaYoYF4N7gVsxWuyhTN2ToHsTIIibjqZSY8qQTRqOvgWF821ubnyL2psrKQQH2zXQ\"\/<\/figure><\/div>\n\n\n

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