{"id":9242,"date":"2019-07-10T11:49:14","date_gmt":"2019-07-10T03:49:14","guid":{"rendered":"http:\/\/mp.weixin.qq.com\/s?__biz=MzI3NjUzOTM1Mg==&#038;mid=100010707&#038;idx=1&#038;sn=13a67c4cc9493c533a5067b714c291e9&#038;chksm=6b7158645c06d1720270018d98ec328486070a9eb4c370290b171cf9da104476c97e7d4249c4#rd"},"modified":"2026-04-08T10:19:50","modified_gmt":"2026-04-08T02:19:50","slug":"10152100","status":"publish","type":"post","link":"https:\/\/www.geneclub.net.cn\/index.php\/2019\/07\/10\/10152100\/","title":{"rendered":"\u201c\u57fa\u56e02.0\u65f6\u4ee3\u201d\u7684\u6838\u5fc3\u6280\u672f\uff1a\u57fa\u56e0\u5408\u6210\uff08\u4e0b\uff09"},"content":{"rendered":"<p><!-- wechat_content_hash: e4d6021859468331 --><\/p>\n<div>\n\n<div class=\"wp-block-image aligncenter\">\n<figure class=\"\"><img decoding=\"async\" src=\"https:\/\/www.geneclub.net.cn\/wp-content\/uploads\/images\/RmFicvQBAkgCuC54K47siaoibvbClYbpoQdmoEA7Yo9T0l4l5c9wsK8DWv13zXvBYojNDUX4HpbxOEe8enSicCuVVg.gif\" alt=\"RmFicvQBAkgCuC54K47siaoibvbClYbpoQdmoEA7Yo9T0l4l5c9wsK8DWv13zXvBYojNDUX4HpbxOEe8enSicCuVVg\"\/><\/figure><\/div>\n\n<div>\u767e\u5bb6\u8c08<\/div>\n<div>\u751f\u547d\u5065\u5eb7\u7684\u5148\u950b\u58f0\u97f3<\/div>\n<div>\u5173\u952e\u8bcd\/\u57fa\u56e0\u5408\u6210<\/div>\n<div>\u00a0<\/div>\n<div>\u9ad8\u901a\u91cf\u57fa\u56e0\u6d4b\u5e8f\u7b49\u6280\u672f\u201c\u8bfb\u201dDNA\uff0c\u57fa\u4e8e\u9057\u4f20\u4fe1\u606f\u6b63\u5feb\u901f\u7528\u4e8e\u533b\u7597\u5065\u5eb7\u7b49\uff0c\u6b64\u4e3a\u5f53\u4e0b\u201c\u57fa\u56e01.0\u65f6\u4ee3\u201d\uff1b\u800c\u6b63\u5728\u915d\u917f\u7684\u201c\u57fa\u56e02.0\u65f6\u4ee3\u201d\uff0c\u901a\u8fc7\u57fa\u56e0\u5408\u6210 \u201c\u5199\u201dDNA\uff0c\u80fd\u591f\u6df1\u5ea6\u7406\u89e3\u548c\u8bbe\u8ba1\u751f\u7269\u7cfb\u7edf\uff0c\u91cd\u6784\u80b2\u79cd\u548c\u80fd\u6e90\u7b49\u9886\u57df\u3002\u9884\u89c1\u672a\u6765\uff0c\u672c\u6587\u4ece\u6280\u672f\u89d2\u5ea6\u4e00\u89c8\u57fa\u56e0\u5408\u6210\u539f\u7406\u548c\u6d41\u7a0b\uff08\u4e0b\u7bc7\uff09\u3002<\/div>\n<div>* \u6b22\u8fce\u5411\u57fa\u56e0\u6167\u6295\u7a3f\uff0c\u878d\u8d44\u4fe1\u606f\u9996\u53d1\u514d\u8d39\u3002<\/div>\n<div>\u00a0<\/div>\n<div>\u5212\u91cd\u70b9<\/div>\n<div>1. \u4e3b\u8981\u6280\u672f\u5e73\u53f0\uff1a\u7269\u7406\u63a9\u819c\u6cd5\u539f\u4f4d\u5408\u6210\u4eea\u5668\u3001\u5149\u654f\u4fdd\u62a4\u57fa\u56e2\u4ecb\u5bfc\u7684\u5149\u63a7\u539f\u4f4d\u5408\u6210\u4eea\u5668\u3001\u57fa\u4e8e\u7535\u5316\u5b66\u4ecb\u5bfc\u9178\u8131\u4fdd\u62a4\u5408\u6210\u65b9\u6cd5\u7684\u534a\u5bfc\u4f53\u539f\u4f4d\u5408\u6210\u4eea\u5668\u3001\u55b7\u58a8\u5f0f\u6253\u5370DNA\u539f\u4f4d\u5408\u6210\u4eea\u5668\u3001\u57fa\u4e8e\u7845\u7247\u7684\u55b7\u58a8\u5f0f\u5408\u6210\u4eea\u5668\u53ca\u539f\u4f4d\u62fc\u63a5\u6280\u672f\u3002<\/div>\n<div>2.\u4e3b\u8981\u6280\u672f\u6311\u6218\uff1a\u4ea7\u91cf\u8fc7\u4f4e\u3001\u5408\u6210\u957f\u5ea6\u53d7\u9650\u3001\u5408\u6210\u51c6\u786e\u6027\u5c40\u9650\u3001\u6210\u672c\u4ecd\u9700\u4f18\u5316\u3002<\/div>\n<div>3.\u63d0\u9ad8\u5408\u6210\u4fdd\u771f\u6027\u7684\u5bf9\u7b56\uff1a\u9700\u6539\u8fdb\u82af\u7247\u8bbe\u8ba1\u4ee5\u53ca\u5408\u6210\u5de5\u827a\uff0c\u5e76\u5f15\u5165\u989d\u5916\u7684\u9519\u8bef\u7ea0\u6b63\u63aa\u65bd\uff0c\u6765\u964d\u4f4e\u5408\u6210\u9519\u8bef\u7387\u3002<\/div>\n\n<div class=\"wp-block-image aligncenter\">\n<figure class=\"\"><img decoding=\"async\" src=\"https:\/\/www.geneclub.net.cn\/wp-content\/uploads\/images\/RmFicvQBAkgDGqJ25wBjOKiaY8dUKgVawQDE9eia3FiaTO6OShFzPdQY1xASy7U075KU3L7Hrn5zk6CLvD771JW1dQ.jpeg\" alt=\"RmFicvQBAkgDGqJ25wBjOKiaY8dUKgVawQDE9eia3FiaTO6OShFzPdQY1xASy7U075KU3L7Hrn5zk6CLvD771JW1dQ\"\/><\/figure><\/div>\n\n<div style=\"text-align: center;\">\u56fe\u7247\u6765\u81ea\u7f51\u7edc<\/div>\n<div>DNA\u6d4b\u5e8f\u548c\u4eba\u5de5\u5408\u6210\u6280\u672f\u5171\u540c\u63a8\u8fdb\u4e86\u5408\u6210\u751f\u7269\u5b66\u7684\u5feb\u901f\u53d1\u5c55\u3002\u968f\u7740\u6280\u672f\u53d1\u5c55\uff0c\u6d4b\u5e8f\u6210\u672c\u88ab\u6025\u5267\u538b\u7f29\uff0c\u57fa\u56e0\u5408\u6210\u548c\u5be1\u6838\u82f7\u9178\u5408\u6210\u7684\u6210\u672c\u6709\u4e00\u5b9a\u964d\u4f4e\uff0c\u4f46\u5e76\u6ca1\u5448\u73b0\u663e\u8457\u6001\u52bf\uff0c\u4ecd\u6709\u4f18\u5316\u7a7a\u95f4\uff0c\u8fd9\u4e5f\u662f\u89c4\u6a21\u5316\u91cf\u4ea7\u7684\u6838\u5fc3\u56e0\u7d20\u3002<\/div>\n<div>\u5982\u4f55\u89e3\u51b3\u8fd9\u4e2a\u95ee\u9898\uff0c\u6211\u4eec\u5148\u6765\u8ba4\u8bc6DNA\u5408\u6210\u6210\u672c\u53ca\u6280\u672f\u5e73\u53f0\u3002<\/div>\n<div>\u00a0<\/div>\n<div>\u57fa\u56e0\u5408\u6210\u7684\u6210\u672c\u4e00\u822c\u4e0e\u5be1\u6838\u82f7\u9178\u7684\u5408\u6210\u6210\u672c\u76f4\u63a5\u76f8\u5173\u3002\u5341\u591a\u5e74\u6765\uff0c\u5be1\u6838\u82f7\u9178\u7684\u5408\u6210\u6210\u672c\u6ca1\u6709\u660e\u663e\u4e0b\u964d\u3002\u901a\u5e38\u6839\u636e\u5408\u6210\u89c4\u6a21\u3001\u5be1\u6838\u82f7\u9178\u7684\u957f\u5ea6\u548c\u4f9b\u5e94\u5546\u7684\u4e0d\u540c\uff0c\u6bcf\u4e2a\u78b1\u57fa\u7684\u5408\u6210\u6210\u672c\u4e0d\u540c\u3002\u4f20\u7edf\u7684\u4f9b\u5e94\u5546\u8fdb\u884c\u57fa\u56e0\u5408\u6210\u7684\u6210\u672c\u7ea6\u4e3a\u6bcf\u78b1\u57fa0.10\u52300.30\u7f8e\u5143(1kb\u57fa\u56e0\u7684\u6210\u672c\u4e3a100-300\u7f8e\u5143)\uff08Hughes, R.A. and A.D. Ellington 2019\uff09\u3002<\/div>\n<div>\u00a0<\/div>\n<div>\u57fa\u56e0\u5408\u6210\u7684\u4e3b\u8981\u6210\u672c\u6765\u81ea\u5be1\u6838\u82f7\u9178\u5408\u6210\u7684\u8bd5\u5242\u6d88\u8017\uff0c\u6b64\u5916\uff0cDNA\u5408\u6210\u8fd8\u662f\u4e00\u4e2a\u52b3\u52a8\u5bc6\u96c6\u578b\u7684\u8fc7\u7a0b\u3002\u56e0\u6b64\uff0c\u51cf\u5c11\u5408\u6210\u8bd5\u5242\u6d88\u8017\uff0c\u63d0\u9ad8\u901a\u91cf\uff0c\u63d0\u9ad8\u57fa\u56e0\u88c5\u914d\u8fc7\u7a0b\u7684\u81ea\u52a8\u5316\u548c\u51c6\u786e\u6027\uff0c\u624d\u80fd\u663e\u8457\u964d\u4f4e\u57fa\u56e0\u5408\u6210\u7684\u7efc\u5408\u6210\u672c\u3002\u57fa\u4e8e\u5fae\u9635\u5217\u7684\u5be1\u6838\u82f7\u9178\u5408\u6210\u6280\u672f\uff0c\u4ee5\u53ca\u5bf9\u5e94\u7684\u4e0b\u6e38DNA\u7247\u6bb5\u7ec4\u88c5\u6280\u672f\u7684\u53d1\u5c55\uff0c\u6b63\u662f\u663e\u8457\u964d\u4f4e\u57fa\u56e0\u5408\u6210\u6210\u672c\u7684\u5e0c\u671b\u6240\u5728\u3002<\/div>\n<div>\u00a0<\/div>\n<div>\u00a0\u4e00\u00a0\u00a0\u00a0\u5be1\u6838\u82f7\u9178\u5fae\u9635\u5217\u5408\u6210<\/div>\n<div>1\uff09 \u4e3b\u8981\u6280\u672f\u5e73\u53f0<\/div>\n\n<div class=\"wp-block-image aligncenter\">\n<figure class=\"\"><img decoding=\"async\" src=\"https:\/\/www.geneclub.net.cn\/wp-content\/uploads\/images\/RmFicvQBAkgAtSgvIF2U1FibO11LzMlgICEEvMcwiaktpaEd90Ulls206c4mEgONWRqnHibIYsib7AxNBC3VH8X7pPQ.png\" alt=\"RmFicvQBAkgAtSgvIF2U1FibO11LzMlgICEEvMcwiaktpaEd90Ulls206c4mEgONWRqnHibIYsib7AxNBC3VH8X7pPQ\"\/><\/figure><\/div>\n\n<div style=\"text-align: center;\">\u56fe1\uff1a\u5be1\u6838\u82f7\u9178\u56fa\u76f8\u5408\u6210\u65b9\u6cd5\u3002A.\u67f1\u5f0f\u5408\u6210\uff1bB.\u5fae\u9635\u5217\u5408\u6210\u3002\uff08\u6765\u6e90\uff1aCold Spring Harb Perspect Biol\uff09<\/div>\n<div>\u5fae\u9635\u5217\u5be1\u6838\u82f7\u9178\u5408\u6210\u6280\u672f\uff0c\u4e5f\u88ab\u79f0\u4e3a\u82af\u7247\u5408\u6210\u6280\u672f (\u56fe1B) \uff0c\u6700\u521d\u7528\u4e8e\u8bca\u65ad\uff0c\u73b0\u5728\u5df2\u7ecf\u6210\u4e3a\u4e00\u79cd\u6781\u6709\u5e0c\u671b\u4f4e\u6210\u672c\u66ff\u4ee3\u67f1\u5f0f\u5be1\u6838\u82f7\u9178\u5408\u6210\u7684\u65b9\u6cd5\u3002Affymetrix\u516c\u53f8\u662f\u8fd9\u4e00\u9886\u57df\u7684\u65e9\u671f\u5148\u9a71\u4e4b\u4e00\uff0c\u5f00\u53d1\u4e86\u7269\u7406\u63a9\u819c\u6cd5\u539f\u4f4d\u5408\u6210\u4eea\u5668(Fodor et al. 1991;Pease et al. 1994) \uff0c\u91c7\u7528\u5149\u6d3b\u5316\u5316\u5b66\u6280\u672f\uff0c\u5229\u7528\u6807\u51c6\u63a9\u819c\u5149\u523b\u6280\u672f\u5728\u82af\u7247\u8868\u9762\u9009\u62e9\u6027\u5730\u8131\u4fdd\u62a4\u7279\u6b8a\u7684\u5149\u4e0d\u7a33\u5b9a\u6027\u6838\u82f7\u4e9a\u78f7\u9170\u80fa\u5355\u4f53\uff0c\u4ece\u800c\u63a7\u5236\u4e0d\u540c\u533a\u57df\u7684\u5be1\u6838\u82f7\u9178\u5408\u6210\u3002<\/div>\n\n<div class=\"wp-block-image aligncenter\">\n<figure class=\"\"><img decoding=\"async\" src=\"https:\/\/www.geneclub.net.cn\/wp-content\/uploads\/images\/RmFicvQBAkgAtSgvIF2U1FibO11LzMlgICKDk1LKgAIdbxmbBDC7nibnvrcPnJ62Hex5BAM1NmAptKFPRVHnuMdmg.png\" alt=\"RmFicvQBAkgAtSgvIF2U1FibO11LzMlgICKDk1LKgAIdbxmbBDC7nibnvrcPnJ62Hex5BAM1NmAptKFPRVHnuMdmg\"\/><\/figure><\/div>\n\n<div style=\"text-align: center;\">\u56fe2\uff1a\u4e3b\u8981\u5fae\u9635\u5217\u5408\u6210\u6280\u672f\u5e73\u53f0\u3002\uff08\u6765\u6e90\uff1a\u4f5c\u8005\uff09<\/div>\n<div>Roche Nimblegen\u516c\u53f8\u548cLC Sciences\u516c\u53f8\uff08\u8054\u5ddd\u751f\u7269\u56fd\u9645\u7814\u53d1\u4e2d\u5fc3\uff09\u7b80\u5316\u4e86\u5149\u4ecb\u5bfc\u7684\u5408\u6210\u8fc7\u7a0b\uff0c\u5c06\u5149\u63a7\u65b9\u6cd5\u4ece\u7269\u7406\u63a9\u819c\u5347\u7ea7\u5230\u6570\u5b57\u5316\u63a7\u5236\u65b9\u6cd5\uff0c\u5f00\u53d1\u4e86\u5149\u654f\u4fdd\u62a4\u57fa\u56e2\u4ecb\u5bfc\u7684\u5149\u63a7\u539f\u4f4d\u5408\u6210\u4eea\u5668(Singh-Gasson et al. 1999;Gao et al. 2001)\u3002<\/div>\n<div>\u00a0<\/div>\n<div>CustomArray\u516c\u53f8\u5f00\u53d1\u4e86\u57fa\u4e8e\u7535\u5316\u5b66\u4ecb\u5bfc\u9178\u8131\u4fdd\u62a4\u5408\u6210\u65b9\u6cd5\u7684\u534a\u5bfc\u4f53\u539f\u4f4d\u5408\u6210\u4eea\u5668\uff08Egeland, R.D. et al. 2002; Ghindilis, A.L., et al.2007\uff09\uff0c\u53ef\u4f7f\u7528\u6807\u51c6\u7684\u4e9a\u78f7\u9170\u80fa\u5355\u4f53\u548c\u76f8\u5173\u8bd5\u5242\u8fdb\u884cDNA\u5408\u6210\uff0c\u907f\u514d\u4e86\u6602\u8d35\u7684\u7269\u7406\u63a9\u819c\u6216\u5149\u5b66\u4eea\u5668\u3002Agilent\u516c\u53f8\u7814\u53d1\u7684\u55b7\u58a8\u5f0f\u6253\u5370DNA\u539f\u4f4d\u5408\u6210\u4eea\u5668\u5219\u91c7\u7528\u975e\u63a5\u89e6\u5f0f\u5de5\u4e1a\u55b7\u58a8\u5370\u5237\u5de5\u827a\uff0c\u56db\u79cd\u78b1\u57fa\u5355\u4f53\u4f5c\u4e3a\u201c\u58a8\u6c34\u201d\u9010\u4e2a\u55b7\u70b9\u5728\u82af\u7247\u4e0a\uff0c\u5b9e\u73b0\u539f\u4f4d\u6253\u5370\u5be1\u6838\u82f7\u9178\u5f15\u7269\uff0c\u8be5\u516c\u53f8\u80fd\u591f\u5408\u6210\u957f\u5ea6\u8d85\u8fc7200\u4e2a\u6838\u82f7\u9178\u7684\u957f\u94fe\u5f15\u7269\uff08Recht, M.I., et al.1996\uff1bHughes, T.R., et al.2001\uff09\u3002Twist\u516c\u53f8\u5f00\u53d1\u4e86\u57fa\u4e8e\u7845\u7247\u7684\u55b7\u58a8\u5f0f\u5408\u6210\u4eea\u5668\u53ca\u539f\u4f4d\u62fc\u63a5\u6280\u672f\uff08\u4e13\u5229US9981239B2, US9677067B2\uff09\u3002<\/div>\n<div>\u00a0<\/div>\n<div>2\uff09\u00a0\u4e3b\u8981\u6280\u672f\u4f18\u52bf\u53ca\u7f3a\u9677<\/div>\n<div>\u57fa\u4e8e\u82af\u7247\u7684\u5be1\u6838\u82f7\u9178\u5fae\u9635\u5217\u5408\u6210\u6280\u672f\u63d0\u4f9b\u4e86\u4f20\u7edf\u67f1\u5f0f\u5408\u6210\u6240\u4e0d\u53ef\u80fd\u5b9e\u73b0\u7684\u591a\u901a\u9053\u5408\u6210\u6a21\u5f0f\u3002\u7531\u4e8e\u6bcf\u5f20\u82af\u7247\u53ef\u80fd\u6709\u4e0a\u4e07\u7684\u5408\u6210\u5bc6\u5ea6\uff0c\u4e00\u4e9b\u5408\u6210\u4eea\u751a\u81f3\u80fd\u591f\u540c\u65f6\u5408\u6210\u591a\u5f20\u82af\u7247\uff0c\u5fae\u9635\u5217\u7684\u5408\u6210\u80fd\u529b\u8fdc\u8fdc\u8d85\u8fc7\u4e86\u6700\u5927\u7684\u67f1\u5f0f\u5408\u6210\u4eea\u5668\u3002\u4f46\u662f\u56e0\u4e3a\u82af\u7247\u5408\u6210\u7684\u5be1\u6838\u82f7\u9178\u7ec4\u5206\u8fc7\u4e8e\u590d\u6742\uff0c\u65e0\u6cd5\u5355\u72ec\u5206\u79bb\uff0c\u5982\u679c\u8fd9\u4e9b\u5be1\u6838\u82f7\u9178\u7528\u4e8eDNA\u5408\u6210\uff0c\u4f1a\u5b58\u5728\u7ec4\u88c5\u80cc\u666f\u8fc7\u4e8e\u590d\u6742\u7684\u6311\u6218\u3002<\/div>\n<div>\u00a0<\/div>\n<div>\u5fae\u9635\u5217\u5408\u6210\u5e73\u53f0\u53ef\u4ee5\u663e\u8457\u63d0\u9ad8\u5be1\u6838\u82f7\u9178\u7684\u5408\u6210\u901a\u91cf\uff0c\u5408\u6210\u6240\u9700\u6d88\u8017\u7684\u8bd5\u5242\u91cf\u663e\u8457\u51cf\u5c11\uff0c\u4ece\u800c\u5927\u5927\u964d\u4f4e\u4e86\u6210\u672c\u3002\u6839\u636e\u5e73\u53f0\u3001\u5be1\u6838\u82f7\u9178\u957f\u5ea6\u548c\u5408\u6210\u89c4\u6a21\u7684\u4e0d\u540c\uff0c\u57fa\u4e8e\u5fae\u9635\u5217\u5e73\u53f0\u5408\u6210\u7684\u5be1\u6838\u82f7\u9178\u6210\u672c\u4ece0.00001\u52300.0001\u7f8e\u5143\u4e0d\u7b49\u3002\u4e0e\u67f1\u5f0f\u5408\u6210\u5be1\u6838\u82f7\u9178\u6bcf\u4e2a\u78b1\u57fa0.05-0.10\u7f8e\u5143\u7684\u6210\u672c\u76f8\u6bd4\uff0c\u82af\u7247\u5408\u6210\u5be1\u6838\u82f7\u9178\u5728\u57fa\u56e0\u5408\u6210\u5e94\u7528\u4e2d\u7684\u4f18\u52bf\u5341\u5206\u660e\u663e\u3002<\/div>\n<div>\u00a0<\/div>\n<div>\u3010\u5bf9\u4e8e\u57fa\u56e0\u5408\u6210\u5e94\u7528\u573a\u666f\uff0c\u4e0d\u5229\u4e4b\u5904\u3011<\/div>\n<div>1.\u5355\u6761\u5be1\u6838\u82f7\u9178\u7684\u4ea7\u91cf\u8fc7\u4f4e\uff0c\u901a\u5e38\u6bd4\u67f1\u5f0f\u5408\u6210\u4f4e2-4\u4e2a\u6570\u91cf\u7ea7\u3002<\/div>\n<div>2.\u5408\u6210\u957f\u5ea6\u53d7\u9650\u3001\u4ea7\u7269\u8fc7\u4e8e\u590d\u6742\uff0c\u5e72\u6270\u540e\u7eed\u7684DNA\u7ec4\u88c5\u8fc7\u7a0b\u3002<\/div>\n<div>3.\u5408\u6210\u51c6\u786e\u6027\u53d7\u9650\uff0c\u5f15\u5165\u66f4\u591a\u5408\u6210\u9519\u8bef\uff0c\u63d0\u9ad8\u9a8c\u8bc1\u548c\u9519\u8bef\u77eb\u6b63\u6210\u672c\u3002<\/div>\n<div>\u00a0<\/div>\n<div>\u00a0\u4e8c\u00a0\u00a0\u00a0\u57fa\u4e8e\u5fae\u9635\u5217\u5be1\u6838\u82f7\u9178\u7684\u57fa\u56e0\u5408\u6210\u7b56\u7565<\/div>\n<div>1\uff09\u00a0\u4e3b\u8981\u6311\u6218\u53ca\u5bf9\u5e94\u89e3\u51b3\u65b9\u6848<\/div>\n<div>\u76ee\u524d\u57fa\u56e0\u7ec4\u5c42\u9762\u7684\u4eba\u5de5\u5408\u6210\u57fa\u672c\u662f\u59cb\u4e8e\u67f1\u5f0f\u5408\u6210\u7684\u5be1\u6838\u82f7\u9178\uff0c\u6216\u8005\u5546\u4e1a\u5408\u6210\u7684\u7ecf\u8fc7\u514b\u9686\u9a8c\u8bc1\u76841-2 kbDNA\u6a21\u5757\uff0c\u65e0\u6cd5\u6210\u719f\u8fd0\u7528\u6210\u672c\u66f4\u4f4e\u3001\u901a\u91cf\u66f4\u9ad8\u7684\u5fae\u9635\u5217\u5408\u6210\u5be1\u6838\u82f7\u9178\uff0c\u539f\u56e0\u5728\u4e8e\u5fae\u9635\u5217\u8fdb\u884cDNA\u7684\u4ece\u5934\u5408\u6210\u62fc\u63a5\u4ecd\u5b58\u5728\u4e00\u7cfb\u5217\u5f85\u89e3\u51b3\u7684\u95ee\u9898\u3002<\/div>\n<div>\u00a0<\/div>\n<div>\u3010\u5f85\u89e3\u51b3\u7684\u95ee\u9898\u3011<\/div>\n<div>1.\u5408\u6210\u957f\u5ea6\u77ed\u3002<\/div>\n<div>2.\u9519\u8bef\u7387\u9ad8\u3002<\/div>\n<div>3.\u5355\u79cd\u5e8f\u5217\u5408\u6210\u4ea7\u91cf\u4f4e\u3002<\/div>\n<div>4.\u5be1\u6838\u82f7\u9178\u5e93\u6210\u5206\u590d\u6742\uff0c\u4e0d\u5229\u4e8e\u62fc\u63a5\u3002<\/div>\n<div>\u4f46\u662f\u8fd9\u4e9b\u95ee\u9898\u4e0d\u662f\u4e0d\u53ef\u903e\u8d8a\u7684\uff08\u56fe3\uff09\u3002\u6bd4\u5982\uff0cChurch\u7814\u7a76\u7ec4\u7b49\u91c7\u7528\u5be1\u6838\u82f7\u9178\u4e9a\u5e93\u6269\u589e\u7684\u7b56\u7565\uff0c\u5e76\u5f15\u5165\u9176\u6cd5\u7ea0\u6b63\u7cfb\u7edf\uff0c\u89e3\u51b3\u4e86\u9519\u8bef\u7387\u3001\u4ea7\u91cf\u548c\u6210\u5206\u590d\u6742\u7684\u96be\u70b9(Kosuri, Eroshenko et al. 2010)\uff1b\u9ad8\u6653\u8fde\u7814\u7a76\u7ec4\u548cChurch\u7814\u7a76\u7ec4\u7b49\u901a\u8fc7\u6269\u589e\u63d0\u9ad8\u5be1\u6838\u82f7\u9178\u6d53\u5ea6\uff0c\u5e76\u901a\u8fc7\u53cd\u5411\u94fe\u6742\u4ea4\u6765\u964d\u4f4e\u9519\u8bef\u7387(Tian, Gong et al. 2004)\uff1b\u7530\u656c\u4e1c\u7814\u7a76\u7ec4\u91c7\u7528\u7269\u7406\u624b\u6bb5\u5c06\u5be1\u6838\u82f7\u9178\u5206\u7ec4\u5230\u4e0d\u540c\u5fae\u5b54\uff0c\u518d\u901a\u8fc7\u539f\u4f4d\u6269\u589e\u62fc\u63a5\uff0c\u6765\u964d\u4f4e\u6210\u5206\u7684\u590d\u6742\u6027(Quan, Saaem et al. 2011)\u3002<\/div>\n\n<div class=\"wp-block-image aligncenter\">\n<figure class=\"\"><img decoding=\"async\" src=\"https:\/\/www.geneclub.net.cn\/wp-content\/uploads\/images\/RmFicvQBAkgAtSgvIF2U1FibO11LzMlgICVVpWpW9pYh8WOStbicxwaEL2TCOUbgZhWhMGfZuFuMjHcMedvhphelw.png\" alt=\"RmFicvQBAkgAtSgvIF2U1FibO11LzMlgICVVpWpW9pYh8WOStbicxwaEL2TCOUbgZhWhMGfZuFuMjHcMedvhphelw\"\/><\/figure><\/div>\n\n<div style=\"text-align: center;\">\u56fe3\uff1a\u5fae\u9635\u5217\u5be1\u6838\u82f7\u9178\u7528\u4e8e\u57fa\u56e0\u5408\u6210\u3002A. \u82af\u7247\u539f\u4f4d\u7ec4\u88c5\uff1bB. \u4e9a\u5e93\u7ec4\u88c5\u7b56\u7565\u3002\uff08\u6765\u6e90\uff1aCold Spring Harb Perspect Biol\uff09<\/div>\n<div>\u00a0<\/div>\n<div>2\uff09 \u5fae\u9635\u5217\u5be1\u6838\u82f7\u9178\u5408\u6210\u53ca\u62fc\u63a5\u4fdd\u771f\u6027\u4f4e<\/div>\n<div>\u5fae\u9635\u5217\u5408\u6210\u5be1\u6838\u82f7\u9178\u7528\u4e8e\u57fa\u56e0\u5408\u6210\u6700\u4e25\u5cfb\u7684\u4e00\u4e2a\u95ee\u9898\u662f\u5fae\u9635\u5217\u5408\u6210\u7684\u5be1\u6838\u82f7\u9178\u8d28\u91cf\u5f80\u5f80\u8f83\u4f4e\uff0c\u4e0e\u67f1\u5f0f\u5408\u6210\u7684\u5be1\u6838\u82f7\u9178\u76f8\u6bd4\u5b58\u5728\u66f4\u591a\u5408\u6210\u9519\u8bef(Kosuri and Church 2014;Wan et al. 2014)\u3002\u00a0<\/div>\n<div>\u00a0<\/div>\n<div>\u5bfc\u81f4\u5408\u6210\u8d28\u91cf\u4e0b\u964d\u7684\u539f\u56e0\u4e4b\u4e00\u662f\u82af\u7247\u4e0a\u5be1\u6838\u82f7\u9178\u5e8f\u5217\u7684\u8131\u560c\u5464\u5316\uff0c\u5728\u5408\u6210\u5468\u671f\u4e2d\uff0c\u7531\u4e8e\u65b0\u751f\u7684\u5be1\u6838\u82f7\u9178\u957f\u65f6\u95f4\u66b4\u9732\u5728\u8131\u4fdd\u62a4\u8bd5\u5242\u4e2d\uff0c\u8131\u560c\u5464\u4f1a\u81ea\u53d1\u53d1\u751f\u3002\u4f18\u5316\u53cd\u5e94\u6761\u4ef6\u548c\u8bd5\u5242\u5728\u5408\u6210\u82af\u7247\u7684\u6d41\u52a8\u5faa\u73af\uff0c\u53ef\u4ee5\u6709\u6548\u63d0\u9ad8\u5be1\u6838\u82f7\u9178\u5408\u6210\u82af\u7247\u7684\u8d28\u91cf\uff0c\u51cf\u5c11\u8131\u560c\u5464\u7684\u53d1\u751f\uff0c\u63d0\u9ad8\u5f97\u7387\uff0c\u8fdb\u800c\u5408\u6210\u957f\u8fbe200nt\u7684\u5be1\u6838\u82f7\u9178(LeProust et al . 2010\u5e74)\u3002\u00a0<\/div>\n<div>\u00a0<\/div>\n<div>\u53e6\u4e00\u4e2a\u539f\u56e0\u662f \u201c\u8fb9\u7f18\u6548\u5e94\u201d\uff0c\u5305\u62ec\u53cd\u5e94\u6db2\u6ef4\u672a\u5bf9\u51c6\uff0c\u8bd5\u5242\u5c01\u95ed\u4e0d\u529b\uff0c\u6216\u5149\u63a7\u7cfb\u7edf\u4e2d\u5149\u675f\u504f\u79fb\u5bfc\u81f4\u4e0d\u7cbe\u786e\u7684\u8131\u4fdd\u62a4\u53cd\u5e94\uff0c\u90fd\u4f1a\u9020\u6210\u8fb9\u7f18\u6548\u5e94\uff0c\u5bfc\u81f4\u90bb\u8fd1\u533a\u57df\u7684\u5be1\u6838\u82f7\u9178\u5e8f\u5217\u4ea7\u751f\u78b1\u57fa\u9519\u8bef\u3002\u5bf9\u82af\u7247\u8bbe\u8ba1\u7684\u6539\u8fdb\u53ef\u4ee5\u63d0\u9ad8\u5fae\u9635\u5217\u5be1\u6838\u82f7\u9178\u7684\u4fdd\u771f\u5ea6(Saaem et al. 2010)\u3002\u56e0\u6b64\uff0c\u5fae\u9635\u5217\u82af\u7247\u8bbe\u8ba1\u548c\u5408\u6210\u8bd5\u5242\u3001\u5de5\u827a\u7684\u4e0d\u65ad\u6539\u8fdb\uff0c\u5c06\u63a8\u52a8\u4f4e\u6210\u672c\u3001\u9ad8\u8d28\u91cf\u3001\u957f\u5be1\u6838\u82f7\u9178\u7684\u9ad8\u4fdd\u771f\u5408\u6210\uff0c\u4e3a\u57fa\u56e0\u5408\u6210\u670d\u52a1\u3002<\/div>\n<div>\u00a0<\/div>\n<div>3\uff09 \u63d0\u9ad8\u5408\u6210\u4fdd\u771f\u6027\u7684\u5bf9\u7b56<\/div>\n<div>\u76ee\u524d\u5fae\u9635\u5217\u5be1\u6838\u82f7\u9178\u76f4\u63a5\u7528\u4e8e\u57fa\u56e0\u5408\u6210\u7684\u6700\u5927\u95ee\u9898\u4f9d\u65e7\u662f\u9519\u8bef\u7387\u8f83\u9ad8\uff0c\u9700\u6539\u8fdb\u82af\u7247\u8bbe\u8ba1\u4ee5\u53ca\u5408\u6210\u5de5\u827a\u6765\u964d\u4f4e\u5408\u6210\u9519\u8bef\u7387(Wan, Li et al. 2014, Lubock, Zhang et al. 2017)\u3002\u5728\u5be1\u6838\u82f7\u9178\u5316\u5b66\u5408\u6210\u8fc7\u7a0b\u4e2d\uff0c\u6bcf\u4e00\u6b65\u5355\u4f53\u6210\u529f\u5076\u8054\u7684\u6548\u7387\u5927\u4e8e\u6216\u7b49\u4e8e98% (Tian, Gong et al. 2004)\u3002\u63d2\u5165\u548c\u7f3a\u5931\u7a81\u53d8\u662f\u8fd9\u4e00\u8fc7\u7a0b\u4e2d\u6700\u5e38\u89c1\u7684\u4e24\u79cd\u7a81\u53d8\u7c7b\u578b\u3002\u7f3a\u5931\u7a81\u53d8\u901a\u5e38\u6765\u81ea\u672a\u5b8c\u6210\u7684\u5076\u8054\u6216\u8005\u8131\u4fdd\u62a4\u6b65\u9aa4\uff0c\u6bcf\u4e2a\u4f4d\u7f6e\u53d1\u751f\u7f3a\u5931\u7a81\u53d8\u7684\u6982\u7387\u5927\u7ea6\u662f5%\u3002\u800c\u63d2\u5165\u7a81\u53d8\u901a\u5e38\u662f\u975e\u9884\u671f\u7684DMT\u5207\u5272\u53cd\u5e94\u5bfc\u81f4\u7684\uff0c\u6bcf\u4e2a\u4f4d\u7f6e\u53d1\u751f\u7684\u6982\u7387\u9ad8\u8fbe0.4% (Hall, Micheletti et al. 2009)\u3002\u9664\u53bb\u8fd9\u4e9b\u975e\u7279\u5f02\u7684\u526f\u4ea7\u7269\uff0c\u82af\u7247\u5408\u6210\u7684100 mer\u5be1\u6838\u82f7\u9178\u4e2d\u53ea\u670930%\u662f\u76ee\u6807\u4ea7\u7269(Tian, Ma et al. 2009)\u3002\u5b58\u5728\u5408\u6210\u9519\u8bef\u7684\u5be1\u6838\u82f7\u9178\u88ab\u5f15\u5165\u57fa\u56e0\u62fc\u63a5\u9636\u6bb5\uff0c\u4f1a\u5c06\u9519\u8bef\u7387\u8fdb\u4e00\u6b65\u653e\u5927\u3002<\/div>\n<div>\u00a0<\/div>\n<div>\u3010\u62fc\u63a5\u8fc7\u7a0b\u9519\u8bef\u7387\u9ad8\u7684\u5e94\u5bf9\u65b9\u6cd5\u3011<\/div>\n<div>1.\u5be1\u6838\u82f7\u9178\u7eaf\u5316\uff1a\u7eaf\u5316\u540e\u7684\u5be1\u6838\u82f7\u9178\u53ef\u4ee5\u5c06\u5408\u6210\u4ea7\u7269\u7684\u4fdd\u771f\u6027\u63d0\u9ad8\u6570\u500d(Xiong, Yao et al. 2006)\u3002\u4f46\u662f\u8fd9\u79cd\u65b9\u6cd5\u53ea\u80fd\u53bb\u9664\u957f\u5ea6\u4e0d\u6b63\u786e\u7684\u9519\u8bef\u5e8f\u5217\uff0c\u65e0\u6cd5\u8bc6\u522b\u78b1\u57fa\u66ff\u6362\u7684\u7a81\u53d8\u7c7b\u578b(Young and Dong 2004)\u3002\u5bf9\u4e8e\u5206\u8fa8\u548c\u53bb\u9664\u5355\u4e2a\u78b1\u57fa\u7f3a\u5931\u6216\u63d2\u5165\u7a81\u53d8\u7684\u5be1\u6838\u82f7\u9178\u4e5f\u5b58\u5728\u4e00\u5b9a\u56f0\u96be\uff0c\u800c\u8fd9\u79cd\u7a81\u53d8\u7c7b\u578b\u6070\u597d\u662f\u57fa\u56e0\u62fc\u88c5\u8fc7\u7a0b\u4e2d\u7684\u4e3b\u8981\u7a81\u53d8\u7c7b\u578b(Tian, Gong et al. 2004)\u3002\u00a0<\/div>\n<div>\u00a0<\/div>\n<div>2.\u529f\u80fd\u7b5b\u9009\uff1a\u5f53\u5408\u6210\u7684\u76ee\u6807DNA\u662f\u7f16\u7801\u5e8f\u5217\u65f6\uff0c\u529f\u80fd\u7b5b\u9009\u53ef\u4ee5\u7528\u6765\u53bb\u9664\u542b\u6709\u7a81\u53d8\u5bfc\u81f4\u5931\u53bb\u529f\u80fd\u7684DNA\u4ea7\u7269\u3002\u5728\u5316\u5b66\u5408\u6210\u5be1\u6838\u82f7\u9178\u7684\u8fc7\u7a0b\u4e2d\uff0cn-1\u7684\u8fb9\u4ea7\u7269\u662f\u5f15\u5165\u7a81\u53d8\u7684\u4e3b\u8981\u7c7b\u578b(Tian, Gong et al. 2004)\u3002\u529f\u80fd\u7b5b\u9009\u65b9\u6cd5\u5bf9\u4e8e\u53bb\u9664\u5be1\u6838\u82f7\u9178\u5316\u5b66\u5408\u6210\u8fc7\u7a0b\u4e2d\u7684\u5355\u4e2a\u78b1\u57fa\u7f3a\u5931\u7a81\u53d8\u7c7b\u578b\u662f\u975e\u5e38\u6709\u6548\u7684\u3002Cox\u7b49\u4eba\u5229\u7528\u8be5\u65b9\u6cd5\uff0c\u5c06\u6b63\u786e\u5408\u6210\u7684DNA\u5e8f\u5217\u7684\u6bd4\u4f8b\u63d0\u9ad8\u4e864\u52305\u500d(Cox, Lape et al. 2007)\u3002\u00a0<\/div>\n<div>\u00a0<\/div>\n<div>3.\u4e8c\u4ee3\u6d4b\u5e8f\u65b9\u6cd5\u9009\u62e9\u6b63\u786e\u5be1\u6838\u82f7\u9178\uff1aMatzas\u7b49\u4eba\u5229\u7528\u7f57\u6c0f454\u6d4b\u5e8f\u6280\u672f\u4e0e\u6311\u62e3\u78c1\u73e0\u7684\u673a\u5668\u4eba\u76f8\u7ed3\u5408\uff0c\u4ece\u6d4b\u5e8f\u5e8f\u5217\u4e2d\u6709\u9009\u62e9\u5730\u53bb\u9664\u5be1\u6838\u82f7\u9178\u5e8f\u5217(\u9644\u7740\u5728\u78c1\u73e0\u4e0a)\uff0c\u5c06\u6b63\u786e\u5e8f\u5217\u7528\u4e8e\u57fa\u56e0\u5408\u6210(Matzas, Stahler et al. 2010)\u3002Kim\u7b49\u4eba\u4f7f\u7528\u968f\u673a\u5e8f\u5217\u6807\u7b7e\u5728454\u6d4b\u5e8f\u53cd\u5e94\u4e2d\u6807\u8bb0\u5355\u4e2a\u5e8f\u5217\uff0c\u5e76\u4f7f\u7528\u8fd9\u4e9b\u6807\u7b7e\u9009\u62e9\u6027\u5730\u6269\u589e\u6b63\u786e\u5e8f\u5217(Kim, Han et al. 2012)\u3002Schwartz\u7b49\u4eba\u91c7\u7528\u7c7b\u4f3c\u7684\u65b9\u6cd5\uff0c\u5229\u7528Illumina\u6d4b\u5e8f\u6761\u7801\uff0c\u901a\u8fc7\u9009\u62e9\u6027\u6269\u589e\u5e8f\u5217\u9a8c\u8bc1\u7684\u6761\u7801\u5e8f\u5217\uff0c\u9009\u51fa\u6b63\u786e\u7684\u5e8f\u5217(Schwartz, Lee et al. 2012)\u30022016\u5e74Klein\u7b49\u4eba\u6539\u8fdb\u4e86\u8fd9\u4e00\u65b9\u6cd5\uff0c\u4ece\u5355\u4e2a\u5be1\u6838\u82f7\u9178\u6c60\u4e2d\u7ec4\u88c5\u4e862271\u6761131-250 bp\u957f\u5ea6\u7684\u7247\u6bb5\uff0c\u5c06\u6b63\u786e\u6bd4\u4f8b\u63d0\u9ad8\u523070.6% (Klein, Lajoie et al. 2016)\u3002\u00a0<\/div>\n<div>\u00a0<\/div>\n<div>4.\u9519\u914d\u8bc6\u522b\u7ea0\u9519\u65b9\u6cd5\uff1a\u9519\u914d\u4fee\u590d\u7cfb\u7edf\u76f8\u5173\u7684\u86cb\u767d\u53ef\u4ee5\u7ef4\u6301\u57fa\u56e0\u7ec4DNA\u590d\u5236\u8fc7\u7a0b\u4e2d\u5e8f\u5217\u7684\u4fdd\u771f\u6027(Modrich 1991)\u3002\u5f15\u5165\u9519\u914d\u8bc6\u522b\u86cb\u767d\u5230\u4eba\u5de5\u5408\u6210DNA\u7684\u7ea0\u9519\u7cfb\u7edf\uff0c\u53ef\u4ee5\u663e\u8457\u63d0\u9ad8DNA\u62fc\u88c5\u4ea7\u7269\u7684\u4fdd\u771f\u6027\u3002<\/div>\n<div>\u00a0<\/div>\n<div>\u57fa\u4e8e\u9519\u914d\u4fee\u9970\u7cfb\u7edf\u76f8\u5173\u86cb\u767d\u6765\u63d0\u9ad8\u5408\u6210\u4fdd\u771f\u6027\u7684\u7b56\u7565\uff0c\u4e00\u79cd\u662f\u9519\u914d\u7ed3\u5408\u86cb\u767d\u7cfb\u7edf\u3002\u9519\u914d\u7ed3\u5408\u86cb\u767d\u53ef\u4ee5\u9009\u62e9\u6027\u5730\u7ed3\u5408\u542b\u6709\u9519\u914d\u7684DNA\u53cc\u94fe\uff0c\u5f62\u6210\u86cb\u767d\u8d28-DNA\u590d\u5408\u4f53\uff0c\u8be5\u590d\u5408\u4f53\u548c\u672a\u7ed3\u5408\u7684\u6b63\u786eDNA\u53ef\u4ee5\u901a\u8fc7\u51dd\u80f6\u963b\u6ede\u5206\u6790\uff08Gel-shift assay\uff09\u6216\u8005\u4eb2\u548c\u67f1\u5206\u79bb\uff0c\u6700\u7ec8\u8fbe\u5230\u9519\u8bef\u53bb\u9664\u7684\u76ee\u7684(Modrich 1991)\u3002Carr\u7b49\u4eba\u6210\u529f\u5f00\u53d1\u7528\u4e8e\u7ea0\u9519\u7cfb\u7edf\uff0c\u5e76\u53ef\u4ee5\u663e\u8457\u63d0\u9ad8DNA\u7684\u4fdd\u771f\u5ea6\uff0c\u5c06\u9519\u8bef\u7387\u4ece1.8 errors\/kb\u964d\u4f4e\u52300.1 error\/kb (Carr, Park et al. 2004, Binkowski, Richmond et al. 2005)\u3002Binkowski, B. F.\u7b49\u4eba\u5219\u5728\u6b64\u57fa\u7840\u4e0a\u4f18\u5316\u4e86\u7ea0\u9519\u7cfb\u7edf\uff0c\u5c06\u86cb\u767d\u8d28\u56fa\u5b9a\u5728\u56fa\u76f8\u57fa\u8d28\u4e0a\uff0c\u7279\u5f02\u6027\u7ed3\u5408\u542b\u6709\u9519\u914d\u7684DNA\u53cc\u94fe\uff0c\u5f62\u6210\u8f83\u5927\u7684\u86cb\u767d\u8d28-DNA\u590d\u5408\u4f53\uff0c\u800c\u540e\u901a\u8fc7\u79bb\u5fc3\u65b9\u6cd5\u53bb\u9664\uff0c\u5f97\u5230\u672a\u7ed3\u5408\u7684\u6b63\u786e\u914d\u5bf9\u7684DNA\u53cc\u94fe\uff0c\u8be5\u65b9\u6cd5\u53ef\u4ee5\u5c06\u7ec4\u88c5\u7684GFPuv\u57fa\u56e0\u5e8f\u5217\u7684\u9519\u8bef\u7387\u4ece1.3 error\/kb\u964d\u4f4e\u52300.3 error\/kb(Binkowski, Richmond et al. 2005)\u3002\u8054\u5ddd\u521b\u59cb\u4eba\u9ad8\u6653\u8fde\u6559\u6388\u8bfe\u9898\u7ec4\u4f7f\u7528MutS\u56fa\u5b9a\u5316\u7ea4\u7ef4\u7d20\u67f1\u6210\u529f\u5c06\u57fa\u4e8e\u82af\u7247\u5be1\u6838\u82f7\u9178\u62fc\u63a5\u7684DNA\u7247\u6bb5\u9519\u8bef\u7387\u964d\u4f4e\u8d85\u8fc720\u500d\uff08\u4ece14.25 error\/kb \u964d\u81f30.66 error\/kb\uff09 (Wan, Li et al. 2014)\u3002\u00a0<\/div>\n<div>\u00a0<\/div>\n<div>\u53e6\u4e00\u79cd\u7528\u4e8e\u7ea0\u9519\u7cfb\u7edf\u7684\u86cb\u767d\u2014\u2014\u9519\u914d\u8bc6\u522b\u5185\u5207\u9176\uff0c\u53ef\u4ee5\u7279\u5f02\u6027\u5730\u8bc6\u522b\u5e76\u5207\u5272\u5f02\u6e90DNA\u53cc\u94fe\u4e0a\u7684\u9519\u914d\u4f4d\u7f6e\u3002\u5207\u5272\u540e\u7684\u5e8f\u5217\u53ef\u4ee5\u8fdb\u884c\u5927\u5c0f\u7b5b\u9009\uff0c\u6216\u52a0\u5165\u53e6\u5916\u7684\u9176\uff0c\u6bd4\u5982DNA\u805a\u5408\u9176\u6216\u8005\u5916\u5207\u9176\u8fdb\u884c\u964d\u89e3\u6216\u8005\u4fee\u590d\u3002\u4fee\u590d\u540e\u7684DNA\u7247\u6bb5\u53ef\u4ee5\u901a\u8fc7\u7247\u6bb5\u4e4b\u95f4\u7684\u91cd\u53e0\u533a\u57df\u91cd\u65b0\u88ab\u5ef6\u957f\u81f3\u76ee\u6807\u957f\u5ea6\u3002Fuhrmann\u7b49\u4eba\u6bd4\u8f83\u4e86\u566c\u83cc\u4f53T4\u5185\u5207\u9176VII\u548c\u5927\u80a0\u6746\u83cc\u5185\u5207\u9176V\u7684\u7ea0\u9519\u6548\u679c\uff0c\u5206\u522b\u53ef\u4ee5\u5c06\u5408\u6210\u57fa\u56e0\u7684\u9519\u8bef\u7387\u4ece6.52 error\/kb\u964d\u4f4e\u52301.62 error\/kb\u548c1.98 error\/kb(Fuhrmann, Oertel et al. 2005)\u3002\u57fa\u4e8e\u7279\u5f02\u6027\u9519\u914d\u8bc6\u522b\u5185\u5207\u9176CEL\u7684\u7ea0\u9519\u7cfb\u7edf\u5df2\u7ecf\u6709\u5546\u4e1a\u5316\u8bd5\u5242\u76d2\uff0c\u53ef\u4ee5\u5c06\u5316\u5b66\u5408\u6210DNA\u4ea7\u7269\u7684\u9519\u8bef\u7387\u964d\u4f4e16\u500d\uff0c\u81f30.11 error\/kb(Kosuri, Eroshenko et al. 2010, Saaem, Ma et al. 2012)\u3002\u4f46\u76ee\u524d\u8fd9\u4e9b\u65b9\u6cd5\u8981\u60f3\u5e94\u7528\u5230\u81ea\u52a8\u5316\u57fa\u56e0\u5408\u6210\u4e2d\uff0c\u5176\u6210\u672c\u3001\u901a\u91cf\u548c\u5de5\u4f5c\u6a21\u5f0f\u4ecd\u9700\u8981\u6539\u8fdb\u3002\u56e0\u6b64\uff0c\u5bf9\u4e8e\u57fa\u4e8e\u5fae\u9635\u5217\u5be1\u6838\u82f7\u9178\u7684DNA\u4ece\u5934\u5408\u6210\u6765\u8bf4\uff0c\u5408\u6210\u57fa\u56e0\u957f\u5ea6\u4e14\u5e8f\u5217\u6b63\u786e\u7684DNA\u7247\u6bb5\u6bd4\u62fc\u63a5\u66f4\u957f\u7247\u6bb5\uff08\u6bd4\u5982\u4ee3\u8c22\u901a\u8def\u6216\u57fa\u56e0\u7ec4\uff09\u66f4\u9700\u8981\u7a81\u7834\u65f6\u95f4\u548c\u6210\u672c\u7684\u9650\u5236\u3002<\/div>\n<div>\u00a0<\/div>\n<div>\u00a0\u4e09\u00a0\u00a0\u00a0\u5c55\u671b<\/div>\n<div>\u5728\u8fc7\u53bb\u768440\u5e74\u4e2d\uff0c\u5316\u5b66\u5408\u6210\u65b9\u6cd5\u7684\u81ea\u52a8\u5316\u53d1\u5c55\u4ee5\u53ca\u57fa\u56e0\u5408\u6210\u65b9\u6cd5\u7684\u53d1\u5c55\u4fc3\u8fdb\u4e86\u6211\u4eec\u5bf9\u751f\u7269\u5b66\u7684\u7406\u89e3\uff0c\u5e76\u4e3a\u751f\u7269\u7cfb\u7edf\u7684\u53ef\u9884\u6d4b\u5de5\u7a0b\u5316\u6539\u9020\u5960\u5b9a\u4e86\u57fa\u7840\u3002\u4eba\u5de5\u5408\u6210\u7684DNA\u5df2\u88ab\u7528\u4e8e\u8bc4\u6d4b\u529f\u80fd\u7ec4\u4ef6(Salis et al.2009\uff1bCallura et al. 2012)\uff0c\u5408\u6210\u57fa\u56e0\u75ab\u82d7(Dormitzer et al.2013)\uff0c\u6784\u5efa\u5408\u6210\u57fa\u56e0\u56de\u8def(Salis et al.2009\uff1bSowa et al. 2015\uff1bNielsen et al. 2016)\uff0c\u57fa\u56e0\u7ec4\u5408\u6210\u4ee5\u53ca\u65b0\u5174\u7684\u201cDNA\u5b58\u50a8\u201d\u9886\u57df\u3002<\/div>\n<div>\u00a0<\/div>\n<div>DNA\u5408\u6210\u6280\u672f\u5728\u751f\u7269\u5de5\u7a0b\u53d1\u5c55\u4e2d\u5904\u4e8e\u6838\u5fc3\u5730\u4f4d\uff0c\u5f53\u4e0e\u7531NGS\u4ea7\u751f\u7684\u5927\u91cf\u5e8f\u5217\u6570\u636e\u76f8\u7ed3\u5408\u65f6\uff0c\u5c06\u6709\u52a9\u4e8e\u5408\u6210\u751f\u7269\u5b66\u5411\u66f4\u5e7f\u9614\u7684\u5e94\u7528\u65b9\u5411\u53d1\u5c55\u3002\u9ad8\u901a\u91cf\u5be1\u6838\u82f7\u9178\u5408\u6210\u6280\u672f\uff0c\u4e0e\u9ad8\u4fdd\u771f\u7ec4\u88c5\u6280\u672f\u3001\u81ea\u52a8\u5316\u6280\u672f\u7684\u7ed3\u5408\uff0c\u4f7f\u5927\u89c4\u6a21\u7684\u5de5\u7a0b\u5316\u6539\u9020\u6210\u4e3a\u53ef\u80fd\u3002\u00a0<\/div>\n<div>\u00a0<\/div>\n<div>\u5f53\u524d\uff0c\u8f83\u77ed\u7684DNA\u7247\u6bb5(1kb)\u901a\u5e38\u53ef\u4ee5\u8f7b\u6613\u5f97\u5230\uff0c\u4f46\u66f4\u957f\u7684DNA\u7247\u6bb5\u7ec4\u88c5\u4ecd\u7136\u5bf9\u64cd\u4f5c\u4eba\u5458\u7684\u6280\u672f\u6c34\u5e73\u63d0\u51fa\u66f4\u9ad8\u8981\u6c42\u548c\u6311\u6218\u3002\u76ee\u6807\u5e8f\u5217\u4e2d\u5b58\u5728\u4e8c\u7ea7\u7ed3\u6784\u4ee5\u53ca\u91cd\u590d\u5e8f\u5217\u90fd\u4f1a\u5bf9\u5176\u7ec4\u88c5\u6784\u6210\u6311\u6218\u3002\u5728DNA\u5408\u6210\u5b8c\u6bd5\u8f6c\u5165\u7ec6\u80de\u65f6\uff0c\u5408\u6210DNA\u7ed3\u6784\u8fd8\u53ef\u80fd\u5bf9\u5bbf\u4e3b\u7ec6\u80de\u9020\u6210\u751f\u957f\u6291\u5236\u3002\u7136\u800c\uff0c\u79d1\u6280\u7684\u8fdb\u6b65\u4e4b\u5feb\u4ee4\u4eba\u548b\u820c\uff0c40\u591a\u5e74\u524d\u7b2c\u4e00\u6761tRNA\u57fa\u56e0\u88ab\u5408\u6210\uff0c2016\u5e74\u7b2c\u4e00\u4e2a\u6700\u5c0f\u7ec6\u83cc\u57fa\u56e0\u7ec4\u88ab\u5408\u6210\u3002\u4eba\u4eec\u5bf9\u751f\u7269\u7cfb\u7edf\u53ca\u5de5\u7a0b\u6539\u9020\u7684\u590d\u6742\u6027\u5df2\u7ecf\u6709\u4e86\u6df1\u523b\u7684\u8ba4\u8bc6\uff0c\u4f46\u672a\u6765\u4ecd\u6709\u8bb8\u591a\u77e5\u8bc6\u7b49\u5f85\u6211\u4eec\u53bb\u63a2\u7d22\u3002<\/div>\n<div>\u6b22\u8fce\u5c31\u6280\u672f\/\u4ea7\u4e1a\u7efc\u8ff0\u7b49\u5411\u57fa\u56e0\u6167\u6295\u7a3f<\/div>\n<div>\u6295\u7a3f\u90ae\u7bb1\uff1ainfo@genonet.cn<\/div>\n<div>\u00a0<\/div>\n<div>\u00a0 \u4f5c\u8005\u7b80\u4ecb\u00a0\u00a0<\/div>\n\n<div class=\"wp-block-image aligncenter\">\n<figure class=\"\"><img decoding=\"async\" src=\"https:\/\/www.geneclub.net.cn\/wp-content\/uploads\/images\/RmFicvQBAkgDGqJ25wBjOKiaY8dUKgVawQ7x23QFAK2GNOSIFg9aibt4PzpECc0VN5pVCZMwRv89hZjthrBcYEetw.jpeg\" alt=\"RmFicvQBAkgDGqJ25wBjOKiaY8dUKgVawQ7x23QFAK2GNOSIFg9aibt4PzpECc0VN5pVCZMwRv89hZjthrBcYEetw\"\/><\/figure><\/div>\n\n<div style=\"text-align: center;\">\u674e\u7490\u7490\u00a0\u00a0\u535a\u58eb<\/div>\n<div>\u674e\u7490\u7490\uff08\u5fae\u4fe1\u53f7\uff1aluannelee\uff09\uff0c\u535a\u58eb\uff0c\u6bd5\u4e1a\u4e8e\u4e2d\u56fd\u79d1\u5b66\u6280\u672f\u5927\u5b66\u751f\u7269\u5316\u5b66\u4e0e\u5206\u5b50\u751f\u7269\u5b66\u4e13\u4e1a\uff0c\u5e08\u4ece\u5408\u6210\u751f\u7269\u5b66\u5148\u9a71\u9ad8\u6653\u8fde\u6559\u6388\uff0c\u4e3b\u8981\u8fdb\u884c\u57fa\u4e8e\u82af\u7247\u5be1\u6838\u82f7\u9178\u7684\u9ad8\u4fdd\u771f\u7ec4\u88c5\u6280\u672f\u5f00\u53d1\uff0c\u6210\u679c\u53d1\u8868\u4e8eNucleic Acids Research \u6742\u5fd7\u30022014\u5e74\u52a0\u5165\u676d\u5dde\u8054\u5ddd\u751f\u7269\uff0c\u73b0\u4efb\u7814\u53d1\u90e8\u4e3b\u7ba1\uff0c\u8d1f\u8d23DNA\u5408\u6210\u6280\u672f\u53caNGS\u57fa\u56e0\u68c0\u6d4b\u6280\u672f\u7684\u5f00\u53d1\u5de5\u4f5c\u3002<\/div>\n<div>\u00a0<\/div>\n<div>\u53c2\u8003\u8d44\u6599\uff1a<\/div>\n<div>1.Binkowski, B. F., K. E. Richmond, J. Kaysen, M. R. Sussman andP. J. Belshaw (2005). &#8220;Correcting errors in synthetic DNA throughconsensus shuffling.&#8221; Nucleic Acids Research 33(6).<\/div>\n<div>2.Carr, P. A., J. S. Park, Y. J. Lee, T. Yu, S. G. Zhang and J. M.Jacobson (2004). &#8220;Protein-mediated error correction for de novo DNAsynthesis.&#8221; Nucleic Acids Research 32(20).<\/div>\n<div>3.Cox, J. C., J. Lape, M. A. Sayed and H. W. Hellinga (2007).&#8221;Protein fabrication automation.&#8221; Protein Science 16(3): 379-390.<\/div>\n<div>4.Fuhrmann, M., W. Oertel, P. Berthold and P. Hegemann (2005).&#8221;Removal of mismatched bases from synthetic genes by enzymatic mismatchcleavage.&#8221; Nucleic Acids Research 33(6).<\/div>\n<div>5.Hall, B., J. M. Micheletti, P. Satya, K. Ogle, J. Pollard and A.D. Ellington (2009). &#8220;Design, synthesis, and amplification of DNA poolsfor in vitro selection.&#8221; Curr Protoc Nucleic Acid Chem Chapter 9: Unit 92.<\/div>\n<div>6.Kim, H., H. Han, J. Ahn, J. Lee, N. Cho, H. Jang, H. Kim, S.Kwon and D. Bang (2012). &#8220;&#8216;Shotgun DNA synthesis&#8217; for the high-throughputconstruction of large DNA molecules.&#8221; Nucleic Acids Res 40(18): e140.<\/div>\n<div>7.Klein, J. C., M. J. Lajoie, J. J. Schwartz, E. M. Strauch, J.Nelson, D. Baker and J. Shendure (2016). &#8220;Multiplex pairwise assembly ofarray-derived DNA oligonucleotides.&#8221; Nucleic Acids Res 44(5): e43.<\/div>\n<div>8.Kosuri, S., N. Eroshenko, E. M. Leproust, M. Super, J. Way, J.B. Li and G. M. Church (2010). &#8220;Scalable gene synthesis by selectiveamplification of DNA pools from high-fidelity microchips.&#8221; Nat Biotechnol28(12): 1295-1299.<\/div>\n<div>9.Lubock, N. B., D. Zhang, A. M. Sidore, G. M. Church and S.Kosuri (2017). &#8220;A systematic comparison of error correction enzymes bynext-generation sequencing.&#8221; Nucleic acids research 45(15): 9206-9217.<\/div>\n<div>10.Matzas, M., P. F. Stahler, N. Kefer, N. Siebelt, V. Boisguerin,J. T. Leonard, A. Keller, C. F. Stahler, P. Haberle, B. Gharizadeh, F.Babrzadeh and G. M. Church (2010). &#8220;High-fidelity gene synthesis byretrieval of sequence-verified DNA identified using high-throughputpyrosequencing.&#8221; Nat Biotechnol 28(12): 1291-1294.<\/div>\n<div>11.Modrich, P. (1991). &#8220;Mechanisms and biological effects ofmismatch repair.&#8221; Annu Rev Genet 25: 229-253.<\/div>\n<div>12.Quan, J., I. Saaem, N. Tang, S. Ma, N. Negre, H. Gong, K. P.White and J. Tian (2011). &#8220;Parallel on-chip gene synthesis and applicationto optimization of protein expression.&#8221; Nat Biotechnol 29(5): 449-452.<\/div>\n<div>13.Saaem, I., S. Ma, J. Quan and J. Tian (2012). &#8220;Errorcorrection of microchip synthesized genes using Surveyor nuclease.&#8221;Nucleic Acids Res 40(3): e23.<\/div>\n<div>14.Schwartz, J. J., C. Lee and J. Shendure (2012). &#8220;Accurategene synthesis with tag-directed retrieval of sequence-verified DNAmolecules.&#8221; Nature Methods 9: 913.<\/div>\n<div>15.Tian, J., H. Gong, N. Sheng, X. Zhou, E. Gulari, X. Gao and G.Church (2004). &#8220;Accurate multiplex gene synthesis from programmable DNAmicrochips.&#8221; Nature 432(7020): 1050-1054.<\/div>\n<div>16.Tian, J. D., K. S. Ma and I. Saaem (2009). &#8220;Advancinghigh-throughput gene synthesis technology.&#8221; Molecular Biosystems 5(7):714-722.<\/div>\n<div>17.Wan, W., L. Li, Q. Xu, Z. Wang, Y. Yao, R. Wang, J. Zhang, H.Liu, X. Gao and J. Hong (2014). &#8220;Error removal in microchip-synthesizedDNA using immobilized MutS.&#8221; Nucleic Acids Res 42(12): e102.<\/div>\n<div>18.Xiong, A. S., Q. H. Yao, R. H. Peng, H. Duan, X. Li, H. Q. Fan,Z. M. Cheng and Y. Li (2006). &#8220;PCR-based accurate synthesis of long DNAsequences.&#8221; Nature Protocols 1(2): 791-797.<\/div>\n<div>19.Young, L. and Q. H. Dong (2004). &#8220;Two-step total genesynthesis method.&#8221; Nucleic Acids Research 32(7).<\/div>\n<div>20.Xie, Z.-X., et al., &#8220;Perfect&#8221; designer chromosome Vand behavior of a ring derivative. Science, 2017. 355(6329).<\/div>\n<div>21.Shen, Y., et al., Deep functional analysis of synII, a770-kilobase synthetic yeast chromosome. Science, 2017. 355(6329).<\/div>\n<div>22.Wu, Y., et al., Bug mapping and fitness testing of chemicallysynthesized chromosome X. Science, 2017. 355(6329).<\/div>\n<div>23.Zhang, W., et al., Engineering the ribosomal DNA in a megabasesynthetic chromosome. Science, 2017. 355(6329).<\/div>\n<div>24.Hughes, R.A.and A.D. Ellington, Synthetic DNASynthesis and Assembly: Putting the Synthetic in Synthetic Biology. ColdSpring Harb Perspect Biol, 2017. 9(1).<\/div>\n<div>\u00a0<\/div>\n<div>\u00a0\u62d3\u5c55\u9605\u8bfb\u00a0<\/div>\n<div><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzI3NjUzOTM1Mg==&amp;mid=2247494342&amp;idx=1&amp;sn=79eff87183ea7d4590ab6924dc97a19d&amp;chksm=eb715871dc06d167406f217f7bf6a18af24e6654fdc550d6a940d089d809b57038ff4198c3f0&amp;scene=21#wechat_redirect\">\u201c\u57fa\u56e02.0\u65f6\u4ee3\u201d\u7684\u57fa\u56e0\u5408\u6210\u6280\u672f\u5e72\u8d27\uff08\u4e0a\uff09<\/a><\/div>\n<div><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzIzMzE4MzU3NA==&amp;mid=2651632901&amp;idx=1&amp;sn=daff78f75065bb8cfe8683dc81ac9541&amp;chksm=f371953cc4061c2a3d8ba8918db022562ebef0ef637e6fec8d784db97e5e51cd2e597d6071f4&amp;scene=21#wechat_redirect\">\u6234\u4fca\u5f6a\u535a\u58eb\u8c08\u5408\u6210\u57fa\u56e0\u7ec4<\/a><\/div>\n<div><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzIzMzE4MzU3NA==&amp;mid=2651632998&amp;idx=1&amp;sn=bd9ba768c58628edef24e0b1b84fd921&amp;chksm=f371955fc4061c49e285b2e702d9782df61cd8d9fdb0e57a9adf57eea4d78fa44608d21b1cce&amp;scene=21#wechat_redirect\">\u4e00\u5957PPT\u6d45\u8c08\u5408\u6210\u57fa\u56e0\u7ec4\u6280\u672f\u548c\u4ea7\u4e1a<\/a><\/div>\n<div>\u58f0\u660e\uff1a\u4ee5\u4e0a\u6587\u7ae0\u4ec5\u4f9b\u7814\u7a76\u53c2\u8003\uff0c\u4e0d\u4f5c\u4e3a\u4e34\u5e8a\u7b49\u51b3\u7b56\u4f9d\u636e\uff1b\u7f16\u8f91\u540e\u7684\u6587\u7ae0\u7684\u77e5\u8bc6\u7248\u6743\u5c5e\u4e8e\u57fa\u56e0\u6167\uff0c\u673a\u6784\u8f6c\u8f7d\u8bf7\u5728\u6587\u7ae0\u4f5c\u8005\u4ee5\u53ca\u6b63\u6587\u9996\u884c\u663e\u8457\u6ce8\u660e\u201c\u8f6c\u8f7d\u81ea&#8217;\u57fa\u56e0\u6167&#8217;\u201d\uff0c\u6b22\u8fce\u4e2a\u4eba\u8f6c\u53d1\u81f3\u670b\u53cb\u5708\u53ca\u7fa4\u5206\u4eab\u3002<\/div>\n<div>\u00a0<\/div>\n<div>\u00a0\u6b22\u8fce\u52a0\u5165\u57fa\u56e0\u6167\u00a0<\/div>\n<div>1. 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\u5546\u52a1\u7ecf\u7406<\/div>\n<div>\u5546\u52a1\u9879\u76ee\u7ba1\u7406\u53ca\u5408\u4f5c\u5f00\u62d3\uff0c\u5173\u8054\u884c\u4e1a\u5e02\u573a\/\u9500\u552e\u7ecf\u9a8c1y+<\/div>\n<div>\uff08\u7b80\u5386\u6295\u9012\u90ae\u7bb1\uff1ainfo@genonet.cn)<\/div>\n<div>\u00a0<\/div>\n<div>\u3010\u5173\u4e8e\u57fa\u56e0\u6167\u3011\u6df1\u5733\u57fa\u56e0\u754c\u79d1\u6280\u54a8\u8be2\u6709\u9650\u516c\u53f8\u662f\u4e00\u5bb6\u7b2c\u4e09\u65b9\u57fa\u56e0\u548c\u6570\u5b57\u751f\u547d\u5065\u5eb7\u4ea7\u4e1a\u521b\u65b0\u670d\u52a1\u673a\u6784\uff0c\u6210\u7acb\u4e8e2016\u5e74\uff0c\u57fa\u4e8e10\u5e74\u4ea7\u4e1a\u5b9e\u8df5\u7684\u56e2\u961f\uff0c\u6211\u4eec\u5efa\u7acb\u4e86\u884c\u4e1a\u6570\u636e\u5e93YourMap\u548c\u4e13\u4e1a\u5a92\u4f53\u54c1\u724c\u57fa\u56e0\u6167\uff08GeneClub\uff09\uff0c\u63d0\u4f9b\u4ea7\u4e1a\u89c4\u5212\u3001\u54a8\u8be2\u57f9\u8bad\u548c\u79d1\u6280\u5a92\u4f53\u7b49\u670d\u52a1\uff0c\u81f4\u529b\u4e8e\u5efa\u7acb\u6570\u5b57\u751f\u547d\u5065\u5eb7\u4ea7\u4e1a\u4fe1\u606f\u5927\u6570\u636e\u5e73\u53f0\u3002<\/div>\n<div>\u00a0<\/div>\n<div>\u56fd\u5bb6\u53d1\u6539\u59d4\u300a2019\u5e74\u6218\u7565\u6027\u65b0\u5174\u4ea7\u4e1a\u53d1\u5c55\u5c55\u671b\u300b\u7f16\u59d4\u7684\u5355\u4f4d<\/div>\n<div>\u5168\u56fd\u536b\u751f\u4ea7\u4e1a\u4f01\u4e1a\u7ba1\u7406\u534f\u4f1a\u7cbe\u51c6\u533b\u7597\u5206\u4f1a\u7406\u4e8b\u7684\u5355\u4f4d<\/div>\n<div>\u54c8\u5c14\u6ee8\u5de5\u4e1a\u5927\u5b66\uff08\u6df1\u5733\uff09\u300a\u751f\u7269\u4ea7\u4e1a\u5206\u6790\u300b\u8bfe\u7a0b\u5408\u4f5c\u5355\u4f4d<\/div>\n<div><a 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href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzI3NjUzOTM1Mg==&amp;mid=2247490769&amp;idx=1&amp;sn=36fa39011eb7dd4b148b072d1aadb14c&amp;chksm=eb72ae66dc052770f2c4fc77a777dff121302dbf954b941b54da1d7eaf7eca1fdce86097ee84&amp;scene=21#wechat_redirect\">\u4e3b\u529e\u9996\u5c4a\u7ca4\u6e2f\u6fb3\u5927\u6e7e\u533a\u751f\u547d\u5065\u5eb7\u521b\u65b0\u8bba\u575b<\/a><\/div>\n<div><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzI3NjUzOTM1Mg==&amp;mid=2247485809&amp;idx=1&amp;sn=ed212d2f2640470ee7269e9959868dcc&amp;chksm=eb72bbc6dc0532d0fea4edea3a9b2aaf23affe391982bdf651b0322120844c1546119d1d475a&amp;scene=21#wechat_redirect\">\u534f\u529e\u7b2c\u4e00\u5c4a<\/a>\u548c<a 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