{"id":9174,"date":"2019-12-20T16:44:31","date_gmt":"2019-12-20T08:44:31","guid":{"rendered":"http:\/\/mp.weixin.qq.com\/s?__biz=MzI3NjUzOTM1Mg==&mid=100012184&idx=1&sn=ca4eaf55c0aa5f66ecede917fadbcf5c&chksm=6b71422f5c06cb394f86ccbb63ed38791a2c1e4723ecf1189d2a87a6866f6d59d68c3939ebf5#rd"},"modified":"2026-04-02T11:04:30","modified_gmt":"2026-04-02T03:04:30","slug":"999953","status":"publish","type":"post","link":"https:\/\/www.geneclub.net.cn\/index.php\/2019\/12\/20\/999953\/","title":{"rendered":"\u4ec5\u970045\u5206\u949f\uff01Nanopore\u6d4b\u5e8f\u5b9e\u73b0HPV&\u5bab\u9888\u5fae\u751f\u7269\u7ec4\u540c\u6b65\u68c0\u6d4b"},"content":{"rendered":"

<\/p>\n

\u5173\u952e\u8bcd\/Nanopore\u6d4b\u5e8f \u5fae\u751f\u7269<\/div>\n
\u6765\u6e90\/\u5143\u7801\u57fa\u56e0 \u7f16\u8f91\/\u57fa\u56e0\u6167<\/div>\n
\u5143\u7801\u57fa\u56e0\u79d1\u5b66\u56e2\u961f\u91c7\u7528\u4e09\u4ee3\u6d4b\u5e8f\u6280\u672f\uff0c\u5feb\u901f\u81f345\u5206\u949f\u5c31\u53ef\u83b7\u5f97\u5bab\u9888\u5904\u524d\u5341\u79cd\u4e3b\u8981\u5fae\u751f\u7269\u4e0eHPV\u7684\u4fe1\u606f\u3002\u7814\u7a76\u6210\u679c\u5df2\u4e8e\u5317\u4eac\u65f6\u95f412\u670819\u65e5\u5728Scientific reports (IF=4.122)\u4ee5\u9898\u76ee\u201cSimultaneous detection and comprehensive analysis of HPV and microbiome status of a cervical liquid-based cytology sample using Nanopore MinION sequencing\u201d\u520a\u51fa\u3002<\/div>\n
\u00a0<\/div>\n
\u4eba\u4f53\u5185\u73af\u5883\u662f\u590d\u6742\u7684\u5927\u81ea\u7136\u4e2d\u4e00\u4e2a\u76f8\u5bf9\u72ec\u7acb\u7684\u5c0f\u73af\u5883\u3002\u5728\u8fd9\u4e2a\u5c0f\u73af\u5883\u91cc\u9762\u6709\u5927\u91cf\u7684\u5fae\u751f\u7269\u4e0e\u6211\u4eec\u751f\u6d3b\u5728\u4e00\u8d77\uff0c\u5176\u4e2d\u6709\u76ca\u751f\u83cc\u4fdd\u62a4\u6211\u4eec\uff0c\u4e5f\u53ef\u80fd\u6709\u75c5\u539f\u83cc\u4f24\u5bb3\u6211\u4eec\u3002\u4f53\u5185\u5fae\u751f\u7269\u83cc\u7fa4\u7684\u53d8\u5316\u53ef\u80fd\u5f15\u8d77\u67d0\u79cd\u75be\u75c5\uff0c\u540c\u65f6\u8eab\u4f53\u72b6\u6001\u4e5f\u53ef\u80fd\u53cd\u8fc7\u6765\u4f5c\u7528\u4e8e\u83cc\u7fa4\u3002\u4eba\u7c7b\u4e73\u5934\u7624\u75c5\u6bd2\uff08human papillomavirus, HPV\uff09\u662f\u5f15\u8d77\u5bab\u9888\u764c\u7684\u4e3b\u8981\u75c5\u539f\u751f\u7269\uff0c\u540c\u65f6\u5bab\u9888\u764c\u7684\u53d1\u5c55\u53ef\u80fd\u4e0e\u5bab\u9888\u5904\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u53d8\u5316\u6709\u5173\u3002<\/div>\n
\u00a0<\/div>\n
\u7136\u800c\uff0c\u5e02\u9762\u4e0a\u76ee\u524d\u8fd8\u6ca1\u6709\u4e00\u79cd\u53ef\u4ee5\u540c\u65f6\u5feb\u901f\u68c0\u6d4bHPV\u75c5\u6bd2\u548c\u5176\u4ed6\u5fae\u751f\u7269\u7684\u65b9\u6cd5\u3002\u56e0\u6b64\uff0c\u6211\u4eec\u9700\u8981\u66f4\u597d\u7684\u5de5\u5177\u5bf9HPV\u7b49\u5fae\u751f\u7269\u8fdb\u884c\u533a\u5206\u9274\u522b\u5206\u6790\u3002<\/div>\n\n
\n
\"RmFicvQBAkgCBWx1VDiabyr6qgsEbibGc9oK50KIb12ib6vnmIXTHNtw3JiczY7vlVhUiaDt2zrQvRqWVkKeW5saDIyg\"\/<\/figure><\/div>\n\n
\u56fe1\uff1aHPV\u75c5\u6bd2\uff08\u6765\u6e90\/\u4e92\u8054\u7f51\uff09<\/div>\n
\u9488\u5bf9\u8fd9\u4e2a\u95ee\u9898\uff0c\u5143\u7801\u57fa\u56e0\u79d1\u5b66\u56e2\u961f\u91c7\u7528\u591a\u91cd PCR\u6280\u672f\u548cNanopore\u6d4b\u5e8f\uff0c\u53ea\u9700\u82b1\u8d39 45\u5206\u949f\uff0c\u5c31\u53ef\u4ee5\u540c\u65f6\u5f97\u5230\u5bab\u9888\u5904\u524d\u5341\u79cd\u4e3b\u8981\u5fae\u751f\u7269\u4e0e HPV\u7684\u4fe1\u606f\u3002\u5177\u4f53\u7684\u65b9\u6cd5\uff0c\u91c7\u7528\u591a\u91cd PCR\u6280\u672f\u6269\u589e HPV E6&E7 \u533a\u57df\u4ee5\u53ca 16s \u5168\u957f\uff0c\u5e76\u5bf9\u6269\u589e\u4ea7\u7269\u4f7f\u7528Nanopore\u8fdb\u884c\u6d4b\u5e8f\u3002<\/div>\n
\u00a0<\/div>\n
\u7ecf\u9a8c\u8bc1\uff0c\u5176\u5206\u6790\u7ed3\u679c\u4e0e Illumina \u6d4b\u5e8f\u5e73\u53f0\u68c0\u6d4b\u7ed3\u679c\u4e00\u81f4\uff0c\u4f46\u82b1\u8d39\u7684\u65f6\u95f4\u5927\u5927\u7f29\u77ed\u3002<\/div>\n\n
\n
\"RmFicvQBAkgCBWx1VDiabyr6qgsEbibGc9ouoXxIjJJMeibZyGAROx0y4qBHibNU1VxHItyjj4cObXLvwjqsqG9emzw\"\/<\/figure><\/div>\n\n
\u56fe2\uff1a\u8be5\u9879\u7814\u7a76\u6210\u679c\u5df2\u5728Scientific reports ( IF=4.122) \u4e0a\u520a\u51fa\uff08\u6765\u6e90\/\u5143\u7801\u57fa\u56e0\uff0c\u4e0b\u540c\uff09<\/div>\n
\u8be5\u9879\u7814\u7a76\u6210\u679c\u5df2\u4e8e\u5317\u4eac\u65f6\u95f412\u670819\u65e5\u5728Scientific reports ( IF=4.122)\u4e0a\u520a\u51fa\uff0c\u662f\u76ee\u524d\u7b2c\u4e00\u7bc7\u7528ONT\u5355\u5206\u5b50\u6d4b\u5e8f\u5e73\u53f0\u540c\u65f6\u5feb\u901f\u68c0\u6d4bHPV\u611f\u67d3\u548c\u5bab\u9888\u5fae\u751f\u7269\u7ec4\u7684\u6587\u7ae0\u3002<\/div>\n
\u00a0<\/div>\n
\u4e00\u3001\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784<\/div>\n
\u3010\u5177\u4f53\u65b9\u6cd5\u3011\u5143\u7801\u57fa\u56e0\u56e2\u961f\u5206\u522b\u91c7\u7528Nanopore\u548cIllumina\u6d4b\u5e8f\u5e73\u53f0\u5bf9\u5fae\u751f\u7269\u8fdb\u884c16s\u6269\u589e\u5b50\u6d4b\u5e8f\u3002<\/div>\n
\u25a0 Nanopore\u6d4b\u5e8f\u5e73\u53f0<\/div>\n
\u6d4b\u5e8f\u591a\u91cdPCR\u4ea7\u7269\uff0c\u5f15\u7269\u5206\u522b\u4e3a16s\u5168\u957f\u4e0eHPV E6&E7 \u533a\u3002<\/div>\n
\u25a0 Illumina \u6d4b\u5e8f\u5e73\u53f0<\/div>\n
\u53ea\u68c0\u6d4b16s V4\u533a\u4ea7\u7269\u3002<\/div>\n
\u25a0\u00a0\u6bd4\u5bf9\u548c\u6ce8\u91ca<\/div>\n
Nanopore\u6d4b\u5e8f\u6570\u636e\u5148\u4e0eHPV\u57fa\u56e0\u7ec4\u6570\u636e\u5e93\u6bd4\u5bf9\uff0c\u5f97\u5230\u7684HPV\u4fe1\u606f\u4f5c\u4e3a\u5206\u578b\u9274\u5b9a\u7684\u8f85\u52a9\u4f9d\u636e\u3002\u5bf9\u4e8e\u6ca1\u6709\u6bd4\u5bf9\u4e0aHPV\u7684\u6d4b\u5e8f\u6570\u636e\uff0c\u7ee7\u7eed\u8fdb\u884c\u5fae\u751f\u7269\u7269\u79cd\u6ce8\u91ca\u6d41\u7a0b\u3002<\/div>\n
\u00a0<\/div>\n
16s \u5206\u6790\u7ed3\u679c\u663e\u793a\uff0cNanopore\u6d4b\u5e8f\u5e73\u53f0\u68c0\u6d4b\u5230\u4e30\u5ea6\u524d\u5341\u79cd\u5fae\u751f\u7269\u5728\u4e0d\u540c\u5206\u7c7b\u6c34\u5e73\u4e0eIllumina\u6d4b\u5e8f\u5e73\u53f0\u4e00\u81f4\u3002\u4f4e\u4e30\u5ea6\u7684rare genus\u79cd\u7c7b\u6570\u91cf\uff0cIllumina\u5e73\u53f0\u68c0\u6d4b\u5230\u7684\u6bd4Nanopore\u5e73\u53f0\u591a\uff0c\u5176\u539f\u56e0\u53ef\u80fd\u56e0\u4e3aIllumina\u5e73\u53f0\u6d4b\u5e8f\u6df1\u5ea6\u8fdc\u5927\u4e8eNanopore\u5e73\u53f0\u3002<\/div>\n\n
\n
\"RmFicvQBAkgCBWx1VDiabyr6qgsEbibGc9o11A7hAJsBqaedaQWKl7tmiaDMF2LEEx0CskaTxjWgG6XSNdJ4HuF4mQ\"\/<\/figure><\/div>\n\n<\/div>\n
\n\n
\n
\"RmFicvQBAkgCBWx1VDiabyr6qgsEbibGc9okJPlc9XjC4upaQ6icsP2F0rzELd2AcRtsHbyMXtBKaQSziavwBYzKR4A\"\/<\/figure><\/div>\n\n
\u56fe3\uff1a\u4e0d\u540c\u5206\u7c7b\u6c34\u5e73\u7684\u7269\u79cd\u6ce8\u91ca\uff08a\uff0c\u95e8\u6c34\u5e73\uff1bb\uff0c\u7eb2\u6c34\u5e73\uff1bc\uff0c\u76ee\u6c34\u5e73\uff1bd\uff0c\u79d1\u6c34\u5e73\uff1be\uff0c\u5c5e\u6c34\u5e73\uff09<\/div>\n
\u6b64\u524d\u5df2\u6709\u6587\u732e\u62a5\u9053\uff0c\u540c\u4e00\u6279\u6b21\u6d4b\u5e8f\u6570\u636e\u5982\u4f7f\u7528\u4e0d\u540c\u7684\u5206\u6790\u65b9\u6cd5\u5219\u4f1a\u4ea7\u751f\u4e0d\u540c\u7684\u7ed3\u679c\u3002\u8fd9\u5176\u4e2d\uff0c\u6bd4\u5bf9\u65b9\u6cd5\u4e0e\u4f7f\u7528\u7684\u6570\u636e\u5e93\u6709\u5f88\u5927\u7684\u5f71\u54cd\u3002\u57fa\u4e8e\u6b64\uff0c\u91c7\u7528\u4e86\u4e0d\u540c\u7684\u65b9\u6cd5\u4e0e\u6570\u636e\u5e93\u5206\u522b\u5bf9Nanopore\u6d4b\u5e8f\u6837\u54c1\u8fdb\u884c\u5206\u6790\u3002<\/div>\n
\u00a0<\/div>\n
\u25a0\u91c7\u7528\u4e0d\u540c\u65b9\u6cd5\u4e0e\u6570\u636e\u5e93\u5bf9Nanopore\u6d4b\u5e8f\u6570\u636e\u8fdb\u884c\u5206\u6790\uff1a<\/div>\n
1\uff09\u4f20\u7edf\u65b9\u6cd5 QIIME + greengenes \u6570\u636e\u5e93\uff1b<\/div>\n
2\uff09Nanopore\u6d4b\u5e8f\u5e38\u7528\u6bd4\u5bf9\u8f6f\u4ef6 LAST + greengenes \u6570\u636e\u5e93\uff1b<\/div>\n
3\uff09Nanopore\u6d4b\u5e8f\u5e38\u7528\u6bd4\u5bf9\u8f6f\u4ef6 LAST + NCBI 16s \u6570\u636e\u5e93\u3002<\/div>\n\n
\n
\"RmFicvQBAkgCBWx1VDiabyr6qgsEbibGc9osChcrsdQ2dvl1NTFz6KdkbSKPGShQIDa3ia1d9YgOodAWoBBQaIJpIA\"\/<\/figure><\/div>\n\n
\u56fe4a\uff1a\u4e0d\u540c\u5206\u6790\u65b9\u6cd5\u7684\u6bd4\u8f83\u56fe\uff1b4b \u9ad8\u4e30\u5ea6\u7269\u79cd\u7684\u4eb2\u7f18\u5173\u7cfb<\/div>\n
\u6587\u6c0f\u56fe\uff08\u56fe4a\uff09\u4e3a\u4e09\u79cd\u65b9\u6cd5\u5728\u5c5e\u6c34\u5e73\u4e0a\u7269\u79cd\u6ce8\u91ca\u7ed3\u679c\u7684\u6bd4\u8f83\uff0c\u4e09\u79cd\u989c\u8272\u7684\u5708\u5206\u522b\u4ee3\u8868\u4e09\u79cd\u65b9\u6cd5\uff0c\u4e2d\u95f4\u768410\u8868\u793a\u4e09\u79cd\u65b9\u6cd5\u68c0\u6d4b\u7ed3\u679c\u5171\u670910\u4e2a\u5c5e\uff0c\u5176\u6b63\u597d\u662f\u4e30\u5ea6\u524d\u5341\u7684\u5fae\u751f\u7269\uff0c\u8bf4\u660e Nanopore 16s \u5168\u957f\u6d4b\u5e8f\u7ed3\u679c\u5177\u6709\u5f88\u597d\u7684 robust \u6027\u3002<\/div>\n
\u00a0<\/div>\n
NCBI \u6570\u636e\u5e93\u76f8\u6bd4greengenes \u6570\u636e\u5e93\u5177\u6709\u4e00\u5927\u4f18\u52bf\uff0c\u5b83\u53ef\u4ee5\u6ce8\u91ca\u5230\u79cd\u6c34\u5e73\uff0c\u5728\u540e\u7eed\u4e34\u5e8a\u5e94\u7528\u4e2d\u53ef\u4ee5\u4e3a\u533b\u751f\u63d0\u4f9b\u66f4\u591a\u7684\u4fe1\u606f\u3002\u7cfb\u7edf\u53d1\u80b2\u6811\uff08\u56fe4b\uff09\u4e3a\u6ce8\u91ca\u5230\u79cd\u6c34\u5e73\u7684\u5fae\u751f\u7269\u4ee5\u53ca\u8fdb\u5316\u5173\u7cfb\uff0c\u5305\u542b\u4e86\u4e30\u5ea6\u524d\u5341\u7684\u5341\u4e2a\u5c5e\u3002<\/div>\n
\u00a0<\/div>\n
\u4e8c\u3001\u5feb\u901f\u68c0\u6d4b<\/div>\n
\u6309\u7167\u4e0d\u540c\u7684\u767e\u5206\u6bd4\u968f\u673a\u62bd\u53d6Nanopore reads\uff0c\u4ee5\u6b64\u6a21\u62df\u4e0d\u540c\u6d4b\u5e8f\u65f6\u95f4\u7684\u6ce8\u91ca\u7ed3\u679c\uff0c\u6bcf\u4e2a\u767e\u5206\u6bd4\u5206\u522b\u968f\u673a\u62bd\u53d6\u5341\u6b21\u3002<\/div>\n
\u25a0\u00a0\u6570\u636e\u62bd\u53d6\u4e4b\u540e\u7684\u5206\u6790\u7ed3\u679c\uff08\u88681\uff09<\/div>\n
\u7b2c\u4e00\u5217\uff1a\u62bd\u53d6reads\u7684\u767e\u5206\u6570\uff1b<\/div>\n
\u7b2c\u4e8c\u5217\uff1a\u6d4b\u5e8f\u7684\u65f6\u95f4\uff1b<\/div>\n
\u7b2c\u4e09\u5217\uff1a\u62bd\u53d6\u7684reads\u6570\uff1b<\/div>\n
\u7b2c\u56db\u5217\uff1a\u62bd\u53d6\u7684reads\u4e2d16s\u5e73\u5747reads\u6570\uff1b<\/div>\n
\u7b2c\u4e94\u5217\uff1a\u62bd\u53d6\u7684reads \u4e2d HPV\u5e73\u5747reads\u6570\uff1b<\/div>\n
\u7b2c\u516d\u5217\uff1a\u5fae\u751f\u7269\u4e30\u5ea6\u524d\u5341\u7684\u5e73\u5747reads\u6570\uff1b<\/div>\n
\u7b2c\u4e03\u5217\uff1a\u652f\u6301reads\u5927\u4e8e4\u65f6\u68c0\u6d4b\u5230\u5168\u90e8\u4e30\u5ea6\u524d\u5341\u7684\u6b21\u6570\uff1b<\/div>\n
\u7b2c\u516b\u5217\uff1a\u6bcf\u6b21\u968f\u673a\u62bd\u53d6\u540e\u4e0e\u5168\u90e8\u6d4b\u5e8freads\u7fa4\u843d\u7ed3\u6784\u7684\u76f8\u4f3c\u7a0b\u5ea6\u3002<\/div>\n
\u4ece\u4e2d\u53ef\u4ee5\u770b\u51fa\uff0c\u53ea\u9700\u6d4b\u5e8f45\u5206\u949f\u5c31\u53ef\u4ee5\u5f97\u5230\u5b8c\u6574\u7684\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u4ee5\u53caHPV\u4fe1\u606f\uff0c\u4e0eMiSeq PE250\u5927\u4e8e24\u5c0f\u65f6\u6d4b\u5e8f\u65f6\u95f4\u76f8\u6bd4\u5927\u5927\u7f29\u77ed\u3002<\/div>\n\n
\n
\"RmFicvQBAkgCBWx1VDiabyr6qgsEbibGc9ovYxoZQnUZib06j4YKHyGsc8Cian1Zq10iaOIU8onbTAETATu1IEHQ7LRw\"\/<\/figure><\/div>\n\n
\u88681\uff1a\u968f\u673a\u62bd\u53d6\u6d4b\u5e8f\u6570\u636e\u7684\u5206\u6790\u6bd4\u8f83<\/div>\n
\u4e09\u3001\u5143\u7801\u57fa\u56e0\u4f9d\u6258\u6280\u672f\u5e73\u53f0\u4f18\u52bf<\/div>\n
\u8fd9\u9879\u6280\u672f\u5177\u6709\u5b9e\u9a8c\u6b65\u9aa4\u7b80\u5355\u3001\u6613\u4e8e\u64cd\u4f5c\u4e14\u82b1\u8d39\u65f6\u95f4\u77ed\u7684\u4f18\u52bf\u3002\u5e94\u7528\u5230\u4e34\u5e8a\u68c0\u6d4b\u65f6\uff0c\u53ef\u4ee5\u5927\u5927\u51cf\u5c11\u60a3\u8005\u7b49\u5019\u7ed3\u679c\u7684\u65f6\u95f4\u3002<\/div>\n
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[1] Rebolj, M. et al. Primary cervical screening with high risk human papillomavirus testing: observational study. BMJ 364, l240 (2019).<\/div>\n
[2] Gao, W., Weng, J., Gao, Y. & Chen, X. Comparison of the vaginal microbiota diversity of women with and without human papillomavirus infection: a cross-sectional study. Bmc Infectious Diseases 13, 271\u2013271 (2013).<\/div>\n
[3] Arokiyaraj, S., Seo, S. S., Kwon, M., Lee, J. K. & Kim, M. K. Association of cervical microbial community with persistence, clearance and negativity of Human Papillomavirus in Korean women: a longitudinal study. Scientific reports 8 (2018).<\/div>\n
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