{"id":8901,"date":"2021-06-16T21:00:09","date_gmt":"2021-06-16T13:00:09","guid":{"rendered":"http:\/\/mp.weixin.qq.com\/s?__biz=MzI3NjUzOTM1Mg==&mid=100019308&idx=1&sn=ec516e701c7bdfcc18cb23f216f4e3b9&chksm=6b717edb5c06f7cd8afa2ee49e4c87208a1401bd6ed9bc5f2a89df4183f1655e426160275fd7#rd"},"modified":"2026-04-08T09:15:45","modified_gmt":"2026-04-08T01:15:45","slug":"579850","status":"publish","type":"post","link":"https:\/\/www.geneclub.net.cn\/index.php\/2021\/06\/16\/579850\/","title":{"rendered":"\u6cdb\u764c\u79cd\u65e9\u68c0\u6280\u672fELSA-seq\u83b7\u81ea\u7136\u5b50\u520a\u53d1\u8868 | \u71c3\u77f3\u9ed1\u79d1\u6280\u518d\u6b21\u8fce\u6765\u9ad8\u5149\u65f6\u523b"},"content":{"rendered":"

<\/p>\n

2021\u5e746\u670815\u65e5\uff0c\u71c3\u77f3\u533b\u5b66\u6cdb\u764c\u79cd\u65e9\u68c0\u6280\u672fELSA-seq\u6b63\u5f0f\u5728\u300a\u81ea\u7136\u00b7\u751f\u7269\u533b\u5b66\u5de5\u7a0b\u300b\uff08Nature Biomedical Engineering\uff0c\u5f71\u54cd\u56e0\u5b50\uff1a18.952\uff09\u53d1\u8868\uff1aUltrasensitive detection of circulating tumour DNA via deep methylation sequencing aided by machine learning\uff08\u673a\u5668\u5b66\u4e60\u8f85\u52a9\u7684\u6df1\u5ea6\u7532\u57fa\u5316\u6d4b\u5e8f\u5bf9\u5faa\u73af\u80bf\u7624DNA\u7684\u8d85\u7075\u654f\u68c0\u6d4b\uff09\u3002\u6587\u7ae0\u4e0e\u5317\u4eac\u534f\u548c\u533b\u9662\u548c\u4e0a\u6d77\u80f8\u79d1\u533b\u9662\u7b49\u673a\u6784\u5408\u4f5c\uff0c\u63ed\u79d8\u4e86ELSA-seq\u6280\u672f\u5982\u4f55\u51c6\u786e\u6355\u6349\u8840\u6db2\u4e2d\u6765\u81ea\u65e9\u671f\u80bf\u7624\u7684\u5fae\u5f31\u4fe1\u53f7\u7684\u6280\u672f\u539f\u7406\uff0c\u5e76\u4e14\u901a\u8fc7\u5728\u80ba\u764c\u53cc\u4e2d\u5fc3\u961f\u5217\u4e2d\u7684\u72ec\u7acb\u9a8c\u8bc1\u6570\u636e\uff0c\u5f62\u6210ELSA-seq\u6280\u672f\u5e94\u7528\u4e8e\u6cdb\u764c\u65e9\u68c0\u548c\u7ec4\u7ec7\u6eaf\u6e90\u7684\u80fd\u529b\u80cc\u4e66\u3002<\/div>\n
\u00a0<\/div>\n
\u6587\u7ae0 | \u71c3\u77f3 \u7f16\u8f91 | April \u5ba1\u6838 | Barney<\/div>\n
\u5173\u952e\u8bcd | ELSA-seq \u80bf\u7624\u65e9\u68c0<\/div>\n\n
\n
\"RmFicvQBAkgAI7zuVLElJCianBIglcAOBd15ia98J5xhXsaIBAoaGtgONufmufhuE3uWTByExthFmYqr3DaH9v79w\"\/<\/figure><\/div>\n\n
\u56fe1\uff1aNature Biomedical Engineering\u6587\u7ae0\u53d1\u8868\u622a\u56fe<\/div>\n
\u5171\u4e00\u4f5c\u8005\uff1a\u6881\u4e43\u65b0\u6559\u6388\uff08\u5317\u4eac\u534f\u548c\u533b\u9662\uff09\uff1b\u674e\u51b0\u601d\u535a\u58eb\uff08\u71c3\u77f3\u533b\u5b66\uff09\uff1b\u5171\u540c\u901a\u8baf\u4f5c\u8005\uff1a\u674e\u5355\u9752\u6559\u6388\uff08\u5317\u4eac\u534f\u548c\u533b\u9662\uff09\uff1b\u8d75\u73e9\u6559\u6388\uff08\u4e0a\u6d77\u4ea4\u901a\u5927\u5b66\u9644\u5c5e\u80f8\u79d1\u533b\u9662\uff09\uff0c\u5f20\u4e4b\u5b8f\u535a\u58eb\uff08\u71c3\u77f3\u533b\u5b66\uff09<\/div>\n
\uff08\u6765\u6e90\/Nature Biomedical Engineering\uff09<\/div>\n
\u5206\u5b50\u751f\u7269\u5b66+\u673a\u5668\u5b66\u4e60\uff1a\u63ed\u5f00\u6cdb\u764c\u79cd\u65e9\u68c0\u8c1c\u56e2<\/div>\n
\u8840\u6d46\u6837\u672c\u4e2d\u4f4e\u4e30\u5ea6\u7684\u5faa\u73af\u80bf\u7624DNA\uff08ctDNA\uff09\u4e00\u76f4\u662f\u764c\u75c7\u65e9\u68c0\u7684\u7a81\u7834\u96be\u70b9\uff0c\u968f\u7740\u8868\u89c2\u9057\u4f20\u5b66\u7684\u52a0\u5165\uff0c\u591a\u7ec4\u5b66\u5206\u5b50\u4fe1\u53f7\u4e0e\u673a\u5668\u5b66\u4e60\u7684\u7ed3\u5408\u5f00\u59cb\u8ba9\u6211\u4eec\u89e6\u78b0\u5230\u6cdb\u764c\u79cd\u65e9\u68c0\u7684\u53ef\u80fd\u3002<\/div>\n
\u5728\u4eba\u7c7b\u9762\u4e34\u7684\u4f17\u591a\u764c\u75c7\u4e2d\uff0c\u80ba\u764c\u4e0d\u540c\u4e8e\u5929\u7136\u4f1a\u5411\u8840\u6db2\u4e2d\u91ca\u653e\u66f4\u591a\u6e38\u79bbDNA\u7684\u764c\u79cd\uff0c\u5982\u809d\u764c\u548c\u8840\u6db2\u80bf\u7624\uff0c\u6216\u8005\u4e0e\u6b63\u5e38\u7ec4\u7ec7\u7279\u5f02\u6027\u5dee\u5f02\u975e\u5e38\u660e\u663e\u7684\u764c\u79cd\uff0c\u5982\u80a0\u764c\uff08\u8868\u89c2\u9057\u4f20\u5b66\u53d8\u5316\u66f4\u4e3a\u4e30\u5bcc\uff09\uff0c\u5bfc\u81f4\u80ba\u764c\u65e9\u68c0\u5728\u751f\u7269\u5b66\u5c42\u9762\u5c24\u4e3a\u56f0\u96be\uff1b\u800c\u80ba\u764c\u540c\u65f6\u4f5c\u4e3a\u5168\u7403\u53d1\u75c5\u7387\u548c\u81f4\u6b7b\u7387\u9996\u4f4d\u7684\u764c\u79cd\uff0c\u5f62\u6210\u4e86\u6cdb\u764c\u79cd\u65e9\u68c0\u5e94\u7528\u7684\u74f6\u9888\u3002\u8fd9\u4e5f\u662f\u6211\u4eec\u4e3a\u4ec0\u4e48\u9891\u9891\u770b\u5230\u80a0\u764c\u4e0e\u809d\u764c\u65e9\u68c0\u6280\u672f\u7684\u9a8c\u8bc1\u6570\u636e\uff0c\u4f46\u80ba\u764c\u6570\u636e\u5be5\u5be5\u65e0\u51e0\u3002\u4ece\u4e00\u5b9a\u7a0b\u5ea6\u6765\u8bf4\uff0c\u653b\u7834\u80ba\u764c\u65e9\u68c0\u5c31\u80fd\u8de8\u8d8a\u591a\u764c\u79cd\u65e9\u68c0\u5e94\u7528\u6700\u91cd\u8981\u7684\u4e00\u4e2a\u95e8\u69db\u3002<\/div>\n
ELSA-seq\u6280\u672f\u4e3a\u4f55\u83b7\u5f97Nature\u5b50\u520a\u7684\u53d1\u8868\uff1f\u6280\u672f\u4e0e\u7b97\u6cd5\u7684\u521b\u65b0\u3001\u53ef\u9760\u7684\u72ec\u7acb\u9a8c\u8bc1\u961f\u5217\u662f\u83b7\u5f97\u8ba4\u53ef\u7684\u5173\u952e\u3002<\/div>\n
\u00a0<\/div>\n
\u6cdb\u764c\u79cd\u65e9\u68c0\u9ed1\u79d1\u6280\uff1aELSA-seq\u6280\u672f\u63ed\u79d8<\/div>\n
\u00a0<\/div>\n
\u25a0\u00a0ELSA-seq\u6280\u672f\u63ed\u79d8\u4e4b\u4e00<\/div>\n
\u201c\u71c3\u77f3\u9e70\u773c\u201d \u2013 \u5c06\u5355\u78b1\u57fa\u5206\u8fa8\u7387\u505a\u5230\u6781\u81f4<\/div>\n
\u51ed\u501f\u5355\u78b1\u57fa\u5206\u8fa8\u7387\u7684\u6d4b\u5e8f\u7cbe\u5ea6\uff0c\u5168\u57fa\u56e0\u7ec4\u91cd\u4e9a\u786b\u9178\u76d0\u6d4b\u5e8f\uff08WGBS\uff09\u5df2\u7ecf\u6210\u4e3a\u4e86\u4e1a\u5185\u7532\u57fa\u5316\u6d4b\u5e8f\u7684\u91d1\u6807\u51c6\uff0c\u7136\u800c\u6b64\u65b9\u6cd5\u5bf9DNA\u7684\u7834\u574f\uff0c\u4f7f\u5176\u4e34\u5e8a\u5e94\u7528\u4e25\u91cd\u53d7\u9650\u3002ELSA-seq\u4f9d\u9760\u5206\u5b50\u751f\u7269\u5b66\u548c\u751f\u7269\u4fe1\u606f\u5b66\u7684\u8bbe\u8ba1\u547c\u5e94\uff0c\u5728\u4e0a\u6e38\u5b9e\u73b0\u4e86\u6781\u5c11\u91cf\u7684\u8840\u6d46\u4e2d\u6e38\u79bbDNA\u7684\u9ad8\u6548\u56de\u6536\u548c\u5229\u7528\uff0c\u5728\u4e0b\u6e38\u81f4\u529b\u4e8e\u4fe1\u566a\u7a97\u53e3\u5206\u79bb\u3002<\/div>\n\n
\n
\"RmFicvQBAkgAI7zuVLElJCianBIglcAOBd1p6ATmTR5EibkXd8AljoUkSicDgD5gJSGDdUCZ8eXT5yPFqrgMmqxJQQ\"\/<\/figure><\/div>\n\n
\u56fe2\uff1aELSA-seq\u6280\u672f\u603b\u89c8<\/div>\n
\uff08\u6837\u672c\u5236\u5907\u2192\u6587\u5e93\u5236\u5907\u2192\u6df1\u5ea6\u6d4b\u5e8f\u2192\u964d\u566a\u5904\u7406\u2192\u7279\u5f81\u9009\u62e9\u2192\u673a\u5668\u5b66\u4e60\uff09<\/div>\n
\uff08\u6765\u6e90\/Nature Biomedical Engineering\uff09<\/div>\n\n
\n
\"RmFicvQBAkgAI7zuVLElJCianBIglcAOBdlVB9zQI2H5Apbtka7UIIW9e2iakXVvDM3O4DIsbfib5hdlEMbBgYWibxA\"\/<\/figure><\/div>\n\n
\u56fe3\uff1aELSA-seq\u4e0e\u4e3b\u6d41\u5546\u7528\u8bd5\u5242\u76d2\u6027\u80fd\u6bd4\u5bf9\u6709\u660e\u663e\u7684\u63d0\u5347<\/div>\n
\uff08\u6765\u6e90\/Nature Biomedical Engineering\uff09<\/div>\n
\u7136\u540e\u89e3\u51b3\u5728\u6570\u636e\u5904\u7406\u4e0a\uff0c\u5982\u4f55\u6709\u6548\u51c6\u786e\u7684\u5bf9\u7532\u57fa\u5316\u4fe1\u53f7\u548c\u566a\u97f3\u8fdb\u884c\u5206\u79bb\u3002<\/div>\n
\u7b80\u5355\u7684\u8bf4\uff0c\u5c31\u662f\u901a\u8fc7\u81ea\u4e3b\u5f00\u53d1\u7684\u751f\u4fe1\u6d41\u7a0b\u514b\u670d\u4e86\u76ee\u524d\u6b64\u7c7b\u6570\u636e\u5e38\u6709\u7684\u6587\u5e93\u590d\u6742\u5ea6\u201c\u9ad8\u4f30\u201d\u7684\u6311\u6218\uff0c\u5e76\u901a\u8fc7\u6709\u6548\u6df1\u5ea6\u6d4b\u5e8f\u5927\u5e45\u964d\u4f4e\u4e86\u6280\u672f\u566a\u97f3\u3002\u5982\u56fe\u53ef\u89c1\uff0c\u5229\u7528PCR\u91cd\u590d\u5e8f\u5217\u964d\u566a\u4e4b\u540e\uff0c\u89c2\u5bdf\u5230\u7684\u6bcf\u79cd\u78b1\u57fa\u53d8\u5f02\u65b9\u5411\u7684\u9519\u8bef\u9891\u7387\uff08AF\uff0c0.00003-0.00135\uff09\u5bf9\u6bd4\u964d\u566a\u524d\u964d\u4f4e\u4e86\u51e0\u4e4e10\u500d\u3002<\/div>\n\n
\n
\"RmFicvQBAkgAI7zuVLElJCianBIglcAOBdS3JoDIsp5OZJPt9oNdQibk7WglBGcRm5cuGo3jJOQH3F76iaVF0uJibNw\"\/<\/figure><\/div>\n\n
\u56fe4\uff1a\u5728\u6ca1\u6709\uff08d\uff09\u6216\u6709\uff08e\uff09\u6df1\u5ea6\u6d4b\u5e8f\u964d\u566a\u7684\u60c5\u51b5\u4e0b\uff0c \u4e0d\u540c\u6d4b\u5e8f\u4eea\u5747\u89c2\u6d4b\u5230\u78b1\u57fa\u9519\u8bef\u7387\u964d\u4f4e10\u500d<\/div>\n
\uff08\u6765\u6e90\/Nature Biomedical Engineering\uff09<\/div>\n
\u25a0\u00a0ELSA-seq\u6280\u672f\u63ed\u79d8\u4e4b\u4e8c<\/div>\n
\u201c\u98de\u82b1\u6458\u53f6\u201d \u2013 \u7cbe\u51c6\u7684\u9776\u5411\u6355\u83b7\u53ea\u7559\u4e0b\u6709\u6548\u4fe1\u606f<\/div>\n
ELSA-seq\u7684\u6838\u5fc3\u662f\u5728\u6781\u5fae\u91cf\u7684DNA\u4e2d\u7cbe\u51c6\u6355\u6349\u4e0e\u764c\u75c7\u4fe1\u53f7\u76f8\u5173\u7684\u7532\u57fa\u5316\u7247\u6bb5\uff0c\u5b83\u7684\u72ec\u5230\u4e4b\u5904\u6b63\u662f\u4fe1\u53f7\u7684\u9ad8\u4fdd\u771f\u653e\u5927\u548c\u9ad8\u6548\u7387\u6355\u83b7\u3002<\/div>\n
\u00a0<\/div>\n
\u5728cfDNA\u6295\u5165\u91cf\u4ec5\u4e3a2 ng\u65f6\uff08\u76f8\u5f53\u4e8e1ml\u5065\u5eb7\u4eba\u8840\u6d46\u6837\u672c\u542b\u91cf\uff09\uff0cELSA-seq\u5b9e\u73b0\u4e86DNA\u6269\u589e\u4e0e\u5747\u4e00\u6027\u6355\u83b7\u7684\u4e24\u5168\uff1a60-80% reads\u53ef\u4ee5\u88ab\u51c6\u786e\u7684\u6bd4\u5bf9\u5728\u9776\u5411\u533a\u57df\u4e0a\uff0c\u5e76\u4e1490%\u4ee5\u4e0a\u7684\u533a\u57df\u88ab>200 reads\u8986\u76d6\u3002<\/div>\n\n
\n
\"RmFicvQBAkgAI7zuVLElJCianBIglcAOBdZkC7gsPiaJlbYLOgpvYpoq20DiboeLmMZShmq6A6MEYGCKIUstkm3r1w\"\/<\/figure><\/div>\n\n
\u56fe5\uff1aELSA-seq panel\u8bbe\u8ba1\u548c\u9776\u5411\u6355\u83b7\u6027\u80fd\u8868\u73b0<\/div>\n
\uff08\u6765\u6e90\/Nature Biomedical Engineering\uff09<\/div>\n
\u25a0\u00a0ELSA-seq\u6280\u672f\u63ed\u79d8\u4e4b\u4e09<\/div>\n
\u201c\u501f\u529b\u6253\u529b\u201d\uff1a\u4ecelocus\u5230block\uff0c\u8bde\u751fMBS\uff08methylation block score\uff09\u65b0\u578b\u7b97\u6cd5<\/div>\n
\u4f20\u7edf\u7684DNA\u7532\u57fa\u5316\u5206\u6790\u4e3b\u8981\u57fa\u4e8e\u5355\u72ec\u4f4d\u70b9\u7532\u57fa\u5316\u53d8\u5f02\u6765\u6355\u6349\u4fe1\u53f7\uff08iAF\uff09\uff0c\u8be5\u65b9\u6cd5\u5bf9\u6d4b\u5e8f\u6df1\u5ea6\u548c\u5355\u70b9\u9519\u8bef\u7387\u975e\u5e38\u654f\u611f\u3002\u4e3a\u6b64\uff0cELSA-seq\u5f00\u53d1\u4e86\u4e00\u79cd\u5bf9\u76f8\u90bb\u7532\u57fa\u5316\u4f4d\u70b9\u7684\u57fa\u56e0\u7ec4\u8ddd\u79bb\u548c\u7532\u57fa\u5316\u6c34\u5e73\u8fdb\u884c\u52a0\u6743\u8ba1\u7b97\u7684\u6253\u5206\u7cfb\u7edf\uff0c\u5bf9\u57fa\u56e0\u7ec4\u8fdb\u884c\u533a\u57df\u5212\u5206\uff08\u4e0b\u56fea\uff09\u3002\u901a\u8fc7\u5bf9\u80ba\u764c\u7279\u5f81\u6027\u7684SHOX2\u57fa\u56e0\u7684\u7532\u57fa\u5316\u53d8\u5f02\u68c0\u6d4b\u53ef\u4ee5\u770b\u51fa\uff0c\u533a\u57df\u7edf\u8ba1\u91cf\uff08mAF\uff09\u6bd4\u4f4d\u70b9\u7edf\u8ba1\u91cfiAF\u5177\u6709\u66f4\u597d\u7684\u8bc6\u522b\u80ba\u764c\u4fe1\u53f7\u7684\u80fd\u529b\uff08\u4e0b\u56feb\uff09\u3002<\/div>\n
\u00a0<\/div>\n
\u6b64\u5916\uff0cELSA-seq\u7528\u4e00\u79cd\u65b0\u7684\u6d4b\u91cf\u7edf\u8ba1\u91cf\u201c\u7532\u57fa\u5316\u533a\u57df\u8bc4\u5206\uff08MBS\uff09\u201d\u5e26\u6765\u4e86\u66f4\u9ad8\u7684\u4fe1\u566a\u6bd4\u3002\u5982\u4e0b\u56fed\u6240\u793a\uff0c\u5373\u4f7f\u662f\u4f4e\u81f31\u2030\u7684\u6837\u672c\u63ba\u6bd4\uff0cMBS\u4e5f\u53ef\u4ee5\u5b9e\u73b0\u4e0e\u9634\u6027\u5bf9\u7167\u7684\u660e\u663e\u533a\u5206\u3002<\/div>\n\n
\n
\"RmFicvQBAkgAI7zuVLElJCianBIglcAOBdDiaDR2L1qlFY4Qiak9MgWPoiaKZLiczxbtL2XOeSDCFyGDFHOXtYibzfJRQ\"\/<\/figure><\/div>\n\n
\u56fe6\uff1aELSA-seq\u7532\u57fa\u5316block\u5b9a\u4e49\u548c\u6027\u80fd\u9a8c\u8bc1<\/div>\n
\uff08\u6765\u6e90\/Nature Biomedical Engineering\uff09<\/div>\n
\u8bf4\u4e86\u8fd9\u4e48\u591a\uff0c\u662f\u65f6\u5019\u770b\u770bELSA-seq\u7684\u8868\u73b0\u4e86\uff0c\u5728\u4f4e\u81f3\u4e07\u5206\u4e4b\u4e94\u4ee5\u4e0a\u7684\u764c\u7ec6\u80deDNA\u542b\u91cf\u6837\u672c\u4e2d\uff0cELSA-seq\u53ef\u4ee5\u8fd1\u4e4e\u5b8c\u7f8e\u5730\u5b9a\u91cf\u764c\u75c7\u4fe1\u53f7\uff08r2=0.99\uff09\uff1b\u5728\u764c\u7ec6\u80deDNA\u542b\u91cf\u4f4e\u81f3\u4e07\u5206\u4e4b\u4e00\u65f6\uff0c\u4f9d\u7136\u53ef\u4ee5\u663e\u8457\u533a\u5206\u764c\u75c7\u4e0e\u5bf9\u7167\u6837\u672c\u3002\u5728\u540c\u6837\u7684\u4e00\u7ec4\u6837\u672c\u4e2d\uff0c\u7814\u7a76\u8005\u6bd4\u5bf9\u4e86ELSA-seq\u4e0e\u4ee3\u8868\u6db2\u4f53\u6d3b\u68c0\u6700\u9ad8\u7075\u654f\u5ea6\u7684\u4e24\u79cd\u65b9\u6cd5\u5b66\uff1aNGS\u548cddPCR\uff0cELSA-seq\u5bf9\u6bd4\u540e\u4e24\u8005\u7075\u654f\u5ea6\u8d85\u8d8a10\u500d\u4ee5\u4e0a\uff0c\u5448\u73b0\u4e86\u78be\u538b\u6027\u7684\u4f18\u52bf\u3002<\/div>\n\n
\n
\"RmFicvQBAkgAI7zuVLElJCianBIglcAOBdHg0Bn6kwLfUa0MmTe3KqvZLSPnSEIgkgias7gkon8OzxVuPr32mAicDw\"\/<\/figure><\/div>\n\n
\u56fe7\uff1aELSA-seq\u6027\u80fd\u9a8c\u8bc1\u7ed3\u679c<\/div>\n
\uff08\u6765\u6e90\/Nature Biomedical Engineering\uff09<\/div>\n
\u4f5c\u4e3a ELSA-seq \u7684\u4e34\u5e8a\u6548\u7528\u7684\u6700\u7ec8\u68c0\u9a8c\uff0c\u6765\u81ea\u5317\u4eac\u534f\u548c\u533b\u9662\u548c\u4e0a\u6d77\u80f8\u79d1\u533b\u9662\u7684\u7814\u7a76\u8005\u5206\u522b\u62db\u52df\u4e86\u4e24\u4e2a\u5b8c\u5168\u72ec\u7acb\u7684\u80ba\u764c\u60a3\u8005\u961f\u5217\uff0c\u8fdb\u884c\u4e86\u65e9\u68c0\u6a21\u578b\u7684\u8bad\u7ec3\/\u9a8c\u8bc1\uff0c\u4ee5\u53ca\u9501\u5b9a\u6a21\u578b\u540e\u72ec\u7acb\u961f\u5217\u7684\u5355\u76f2\u6d4b\u8bd5\uff0c\u5b9e\u9a8c\u540c\u65f6\u8bbe\u7f6e\u4e86\u8de8\u6280\u672f\u5e73\u53f0\u5bf9\u6bd4\u4e9a\u7ec4\uff0c\u8fdb\u884c\u7532\u57fa\u5316\u4fe1\u53f7\uff08ELSA-seq\uff09\u4e0e\u7a81\u53d8\u4fe1\u53f7\uff08NGS\uff09\u201c\u5934\u5bf9\u5934\u201d\u7684\u6bd4\u8f83\u3002\u8fd9\u4e2a\u201c\u7ec8\u6781\u5927\u8003\u201d\u7684\u7b54\u5377\u5982\u4e0b\uff1a<\/div>\n
\u00a0<\/div>\n
ELSA-seq\u5b9e\u73b0\u4e86\u6709\u6548\u7684\u4f4e\u9891\u4fe1\u53f7\u5efa\u6a21\u548c\u8fc7\u62df\u5408\u98ce\u9669\u63a7\u5236\uff1a\u72ec\u7acb\u961f\u52171\u548c\u961f\u52172\u5206\u7c7b\u51c6\u786e\u5ea6\uff08AUC: 0.93 vs 0.90\uff09\u9ad8\u5ea6\u4e00\u81f4\uff1b<\/div>\n
\u591a\u53d8\u91cf\u5206\u6790\u663e\u793a\u68c0\u6d4bELSA-seq\u6a21\u578b\u6253\u5206\u7684\u9ad8\u4f4e\u4e0e\u80bf\u7624\u5206\u671f\u3001\u80bf\u7624\u5927\u5c0f\u548c\u7ec4\u7ec7\u5b66\u4e9a\u578b\u9ad8\u5ea6\u76f8\u5173\uff0c\u5176\u4e2d\u80ba\u9cde\u764c\u4e9a\u578b\u5e73\u5747\u5f97\u5206\u663e\u8457\u9ad8\u4e8e\u80ba\u817a\u764c\uff1b<\/div>\n
\u5bf9\u4e8e\u6240\u6709\u7684\u8840\u6d46\u6837\u672c\uff0c\u572896%\u7684\u6574\u4f53\u7279\u5f02\u6027\u4e0b\uff0cIA\u671f-III\u671f\u80ba\u764c\u60a3\u8005\u7684\u7075\u654f\u5ea6\u8fbe\u523052%-81%\uff1b<\/div>\n
\u7532\u57fa\u5316\u4fe1\u53f7\u53ef\u68c0\u6d4b\u5230\u4f4e\u81f30.02%\u7a81\u53d8\u4e30\u5ea6\u7684\u8840\u6db2\u6837\u672c\uff0c\u4e14\u7532\u57fa\u5316\u4fe1\u53f7\u6210\u529f\u8bc6\u522b\u7684\u80ba\u764c\u60a3\u8005\u6570\u91cf\u51e0\u4e4e\u662fNGS\u7a81\u53d8\u4fe1\u53f7\u8bc6\u522b\u7684\u4e24\u500d\uff0848\/66 vs 28\/66\uff09\uff0c\u540c\u65f6\u4fdd\u6301\u540c\u6837\u7684\u8d85\u4f4e\u5047\u9633\u6027\u7387\uff080\/49\uff09\uff0c\u503c\u5f97\u4e00\u63d0\u7684\u662f\uff0c\u4e24\u8005\u7684\u8054\u5408\u68c0\u6d4b\u53ef\u4ee5\u8fbe\u5230\u66f4\u9ad8\u7684\u9633\u6027\u7387\uff0854\/66\uff09\u3002<\/div>\n\n
\n
\"RmFicvQBAkgAI7zuVLElJCianBIglcAOBdZmG4Oqz9fFFqlibnicmnF3uDKyZUjQaHicyffXBA5c6tUSL9Rg4t1u7MQ\"\/<\/figure><\/div>\n\n
\u56fe8\uff1a\u53cc\u4e2d\u5fc3\u4e34\u5e8a\u9a8c\u8bc1\u8def\u7ebf\u8bbe\u8ba1<\/div>\n
\uff08\u6765\u6e90\/Nature Biomedical Engineering\uff09<\/div>\n
\u00a0<\/div>\n
\u6587\u7ae0\u4e4b\u5916\u7684\u6545\u4e8b\uff1a\u71c3\u77f3\u7684\u901f\u5ea6\u548c\u79d1\u6280\u521b\u65b0\u7684\u529b\u91cf<\/div>\n
ELSA-seq\u8bde\u751f\u4e8e2017\u5e74\uff0c\u57282018\u5e74\u7684CSCO\u7b2c\u4e00\u6b21\u53d1\u5e03\u6570\u636e\uff0c\u8ddd\u4eca\u5df2\u6709\u4e09\u5e74\u7684\u65f6\u95f4\u3002\u5728\u8fd9\u7bc7\u6587\u7ae0\u6210\u6587\u3001\u6253\u78e8\u3001\u6295\u7a3f\u3001\u4fee\u56de\u7684\u6f2b\u957f\u7684\u4e09\u5e74\u91cc\uff0c\u71c3\u77f3\u7684\u7814\u53d1\u56e2\u961f\u53c8\u628aELSA-seq\u8fdb\u884c\u4e86\u6570\u6b21\u7684\u8fed\u4ee3\uff0c\u57282020\u5e74\u7684ESMO Asia\u5927\u4f1a\u53e3\u5934\u62a5\u544a\u4e2d\uff0c\u6765\u81ea\u590d\u65e6\u5927\u5b66\u9644\u5c5e\u4e2d\u5c71\u533b\u9662\u7684\u7814\u7a76\u8005\u6c47\u62a5\u4e86\u6700\u65b0\u4e00\u4ee3ELSA-seq\u6280\u672f\u5728\u80ba\u764c\u3001\u80a0\u764c\u3001\u809d\u764c\u3001\u98df\u7ba1\u764c\u3001\u5375\u5de2\u764c\u3001\u4ee5\u53ca\u80f0\u817a\u764c\u516d\u4e2a\u764c\u79cd\u4e2d\u7684\u9a8c\u8bc1\u6570\u636e\uff0cELSA-seq\u7684\u68c0\u6d4b\u7279\u5f02\u6027\u4e5f\u4ece\u672c\u6587\u768496%\u8fdb\u4e00\u6b65\u63a8\u9ad8\u523098%\u4ee5\u4e0a\uff0c\u5e76\u4e14\u5448\u73b0\u4e86\u9ad8\u8fbe82%\u51c6\u786e\u6027\u7684\u7ec4\u7ec7\u6eaf\u6e90\u80fd\u529b\u3002\u5728\u6b64\u57fa\u7840\u4e0a\uff0c\u4e24\u4e2a\u524d\u77bb\u6027\u65e9\u68c0\u4e07\u4eba\u7814\u7a76\u201cPREDICT\u201d\u548c\u201cPRESCIENT\u201d\u4ea6\u5df2\u5f00\u542f\u3002<\/div>\n
\u00a0<\/div>\n
\u672c\u6587\u7684\u4e00\u56fe\u4e00\u8868\u4e00\u5b57\u4e00\u53e5\u8bb0\u5f55\u4e86ELSA-seq\u6700\u521d\u7684\u6a21\u6837\uff0c\u800c\u5728\u6587\u7ae0\u4e4b\u5916\uff0c\u5b83\u5df2\u7ecf\u4ee5\u547c\u5578\u800c\u8fc7\u7684\u901f\u5ea6\u957f\u51fa\u4e86\u672c\u6587\u6240\u63cf\u753b\u7684\u56fe\u666f\u3002\u8fd9\u6b63\u662f\u764c\u75c7\u65e9\u68c0\u6280\u672f\u751f\u957f\u7684\u901f\u5ea6\uff0c\u662f\u71c3\u77f3\u7684\u901f\u5ea6\u3002\u00a0<\/div>\n
\u00a0<\/div>\n
\u672a\u6765\uff0c\u71c3\u77f3\u671f\u5f85\u4e0e\u533b\u5b66\u754c\u7684\u4e13\u5bb6\u4eec\u4e00\u9053\u52aa\u529b\uff0c\u4e00\u5982\u65e2\u5f80\u4e0d\u9057\u4f59\u529b\u5730\u63a8\u52a8\u764c\u75c7\u65e9\u68c0\u4ea7\u54c1\u7684\u7814\u53d1\u548c\u843d\u5730\uff0c\u4e3a\u764c\u75c7\u60a3\u8005\u5927\u5e45\u5ef6\u957f\u751f\u5b58\u65f6\u95f4\u548c\u63d0\u9ad8\u751f\u547d\u8d28\u91cf\u8d21\u732e\u4e2d\u56fd\u79d1\u6280\u521b\u65b0\u7684\u529b\u91cf\u3002<\/div>\n
\u00a0<\/div>\n
\u53c2\u8003\u8d44\u6599\uff1a<\/div>\n
Liang, N., Li, B., Jia, Z. et al. Ultrasensitive detection of circulating tumour DNA via deep methylation sequencing aided by machine learning. Nat Biomed Eng (2021). https:\/\/doi.org\/10.1038\/s41551-021-00746-5<\/div>\n
\u00a0<\/div>\n
\u6269\u5c55\u9605\u8bfb<\/div>\n
\u63e3\u5c11\u5764\uff1a\u9996\u4e2aNGS\u764c\u75c7\u57fa\u56e0\u68c0\u6d4b\u8bd5\u5242\u76d2\u7684\u5ba1\u6279\u4e4b\u8def<\/a><\/div>\n
\u57fa\u56e0\u884c\u4e1a\u53bb\u5e74\u6700\u70ed\u7684\u8d5b\u9053\uff0c\u4eca\u5e74\u53d1\u751f\u4e86\u54ea\u4e9b\u53d8\u5316\uff1f<\/a><\/div>\n
\u4e2d\u56fd\u80bf\u7624\u5b66\u5927\u4f1a\u80bf\u7624\u6807\u5fd7\u5206\u4f1a\u573a\u7b14\u8bb0<\/a><\/div>\n\n
\n
\"640?wx_fmt=jpeg\"\/<\/figure><\/div>\n\n
\u300a2021\u80bf\u7624\u57fa\u56e0\u53ca\u5206\u5b50\u68c0\u6d4b\u84dd\u76ae\u4e66\u300b<\/div>\n
\u57fa\u56e0\u6167\u8054\u5408\u8d5b\u9ed8\u98de\/\u534e\u5927\u57fa\u56e0\/\u6cdb\u751f\u5b50\/\u91d1\u57df\u533b\u5b66\u7b49\u53d1\u8d77<\/div>\n
\u6b22\u8fce\u4e34\u5e8a\u4e13\u5bb6\u53ca\u4f01\u4e1a\u53c2\u4e0e\u5408\u4f5c<\/div>\n
\u3010\u58f0\u660e\u3011\u4e3a\u4e86\u670d\u52a1\u57fa\u56e0\u53ca\u6570\u5b57\u751f\u547d\u5065\u5eb7\u4ea7\u4e1a\u53d1\u5c55\u3001\u79d1\u6280\u63a8\u5e7f\u53ca\u653f\u4ea7\u5b66\u7814\u7528\u8fde\u63a5\uff0c\u57fa\u56e0\u6167\u79c9\u6301\u4e13\u4e1a\u3001\u8d4b\u80fd\u3001\u4e2d\u7acb\u7684\u7406\u5ff5\u6536\u96c6\u3001\u5206\u6790\u3001\u53d1\u5e03\u4fe1\u606f\u6216\u89c1\u89e3\u3002\u4f46\u7531\u4e8e\u65f6\u6548\u6027\u53ca\u884c\u4e1a\u7279\u6b8a\u6027\uff0c\u6240\u520a\u767b\u5185\u5bb9\u4ec5\u4f9b\u7814\u7a76\u53c2\u8003\uff0c\u4e0d\u4f5c\u4e3a\u51b3\u7b56\u4f9d\u636e\uff1b\u672c\u6587\u76f8\u5173\u4fe1\u606f\u4e0d\u4ee3\u8868\u57fa\u56e0\u6167\u7684\u89c2\u70b9\uff1b\u57fa\u56e0\u6167\u5e73\u53f0\u520a\u767b\u7684\u539f\u521b\u5185\u5bb9\u7684\u77e5\u8bc6\u4ea7\u6743\u4e3a\u201c\u57fa\u56e0\u6167\u201d\u5546\u6807\u62e5\u6709\u8005\u53ca\u76f8\u5173\u6743\u5229\u4eba\u6240\u6709\uff1b\u6b22\u8fce\u8f6c\u8f7d\uff0c\u8f6c\u8f7d\u8bf7\u7533\u8bf7\u5e76\u6ce8\u660e\u6765\u6e90\u3002\u6b22\u8fce\u4e2a\u4eba\u53ca\u673a\u6784\u6295\u7a3f\u53ca\u5408\u4f5c\u3002<\/div>\n
\u00a0<\/div>\n
\u00a0\u5173\u4e8e\u57fa\u56e0\u6167\u00a0<\/div>\n
\u57fa\u56e0\u6167\u662f\u4e00\u5bb6\u57fa\u56e0\u53ca\u6570\u5b57\u751f\u547d\u5065\u5eb7\u4ea7\u4e1a\u521b\u65b0\u670d\u52a1\u5e73\u53f0\u3002\u516c\u53f8\u521b\u7acb\u4e8e2016\u5e74\uff0c\u521b\u59cb\u56e2\u961f\u6df1\u8015\u884c\u4e1a\u5341\u4f59\u5e74\uff0c\u79c9\u6301\u201c\u4f7f\u8fde\u63a5\u4ea7\u751f\u4ef7\u503c\u00b7\u7528\u6570\u636e\u770b\u89c1\u672a\u6765\u201d\u7684\u7406\u5ff5\uff0c\u5efa\u7acb\u4e86\u9996\u4e2a\u6570\u5b57\u751f\u547d\u5065\u5eb7\u4ea7\u4e1a\u6570\u5b57\u5316\u5e73\u53f0YourMap\u00ae\uff0c\u8054\u5408\u653f\u7b56\u3001\u4e34\u5e8a\u3001\u79d1\u7814\u53ca\u4ea7\u4e1a\u673a\u6784\u8fde\u7eed\u56db\u5e74\u53d1\u5e03\u884c\u4e1a\u84dd\u76ae\u4e66\uff0c\u4e13\u6ce8\u4ea7\u4e1a\u54a8\u8be2\u53ca\u5e02\u573a\u670d\u52a1\u3002\u4f5c\u4e3a\u56fd\u53d1\u6539\u4ea7\u4e1a\u7814\u7a76\u5408\u4f5c\u5355\u4f4d\uff0c\u57fa\u56e0\u6167\u4e0e90%\u77e5\u540d\u57fa\u56e0\u673a\u6784\u5efa\u7acb\u4e86\u5408\u4f5c\uff0c\u53c2\u4e0e\u7ec4\u7ec7\u53d1\u5e03\u591a\u9879\u884c\u4e1a\u5171\u8bc6\u548c\u56e2\u4f53\u6807\u51c6\uff0c\u670d\u52a1\u751f\u547d\u79d1\u6280\u521b\u65b0\u521b\u4e1a\u3002<\/div>\n
\u2606\u00a0\u56fd\u53d1\u6539\u300a\u6218\u7565\u6027\u65b0\u5174\u4ea7\u4e1a\u53d1\u5c55\u5c55\u671b\u300b\u6267\u7b14\u5355\u4f4d<\/a><\/div>\n
\u2606\u00a0\u53c2\u4e0e\u7ec4\u7ec7\u673a\u6784\u53d1\u5e03\u591a\u9879\u884c\u4e1a\u5171\u8bc6<\/a>\u3001\u56e2\u4f53\u6807\u51c6<\/a><\/div>\n
\u2606\u00a0\u4e2d\u56fd\u9057\u4f20\u5b66\u4f1a\u751f\u7269\u4ea7\u4e1a\u4fc3\u8fdb\u59d4\u5458\u4f1a\u59d4\u5458<\/div>\n
\u2606\u00a0\u5efa\u7acb\u4ea7\u4e1a\u4e1a\u6570\u5b57\u5316\u5e73\u53f0\u4f18\u8109\u901aYourMap<\/a>\u00ae<\/div>\n
\u2606\u00a0\u4e2d\u56fd\u6297\u764c\u534f\u4f1a\u80bf\u7624\u6807\u5fd7\u4e13\u4e1a\u59d4\u5458\u4f1a\u6218\u7565\u5408\u4f5c\u5355\u4f4d<\/a><\/div>\n
\u2606\u00a0\u53d1\u5e03\u6570\u5341\u4efd\u57fa\u56e0\u53ca\u6570\u5b57\u751f\u547d\u5065\u5eb7\u4ea7\u4e1a\u884c\u7814\u62a5\u544a<\/a><\/div>\n
\u2606\u00a0\u7ec4\u7ec7\u57fa\u56e0\u68c0\u6d4b\u8054\u76df\uff08\u7b79\uff09\u9996\u5c4a<\/a>\u3001\u7b2c\u4e8c\u5c4a<\/a>\u4f1a\u8bae<\/a><\/div>\n
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