{"id":8900,"date":"2021-06-17T20:35:25","date_gmt":"2021-06-17T12:35:25","guid":{"rendered":"http:\/\/mp.weixin.qq.com\/s?__biz=MzI3NjUzOTM1Mg==&mid=100019317&idx=1&sn=34c3dbc015569f12f6fe928c100d14bb&chksm=6b717ec25c06f7d4d3909896ef5ee457d988ce18e5a8a50909a75357dc9537c9c20ed71fb9d3#rd"},"modified":"2026-04-08T09:15:37","modified_gmt":"2026-04-08T01:15:37","slug":"579999-2","status":"publish","type":"post","link":"https:\/\/www.geneclub.net.cn\/index.php\/2021\/06\/17\/579999-2\/","title":{"rendered":"\u62a2\u5148\u62a5\u540d\uff1aMatthias\/John Yates\/\u9ec4\u8d85\u5170\u7b49\u86cb\u767d\u8d28\u7ec4\u5b66\u5927\u725b\u7684\u77e5\u8bc6\u76db\u5bb4"},"content":{"rendered":"

<\/p>\n

\u8d3a\u798f\u521d\u6559\u6388\u63d0\u5230\u201c\u7531\u86cb\u767d\u8d28\u7ec4\u5b66\u9a71\u52a8\u7684\u7cbe\u51c6\u533b\u5b66\uff0c\u52bf\u5fc5\u5e26\u6765\u7cbe\u786e\u8bca\u65ad\u4e0e\u7cbe\u51c6\u6cbb\u7597\u7edf\u4e00\u7684\u7b2c\u4e09\u4ee3\u533b\u5b66\u9769\u547d\u201d\u3002\u5e74\u4e2d\u5927\u4fc3\u4e4b\u9645\uff0c\u4f55\u4e0d\u6765\u4e00\u573a\u77e5\u8bc6\u7684\u72c2\u6b22\uff1fMatthias Mann\u3001John R. Yates III\u3001\u9ec4\u8d85\u5170\u6559\u6388\u7b49\u5728\u86cb\u767d\u8d28\u7ec4\u5b66\u9886\u57df\u5982\u96f7\u8d2f\u8033\u7684\u540d\u5b57\uff0c\u5c06\u805a\u9f50\u5728\u5409\u51ef\u57fa\u56e0\u4e0e\u5e03\u9c81\u514b\u8054\u5408\u4e3e\u529e\u7684\u201c\u65b0\u4e00\u4ee3\u86cb\u767d\u8d28\u7ec4\u524d\u6cbf\u6280\u672f\u4e0e\u5e94\u7528\u7814\u8ba8\u4f1a\u201d\uff0c\u4e8e2021\u5e746\u670824\u65e5\u4e0b\u5348\u4e0e\u60a8\u5206\u4eab\u86cb\u767d\u8d28\u7ec4\u5b66\u5e72\u8d27\uff0cenjoy\u3002<\/div>\n
\u6587\u7ae0 | \u5409\u51ef\u57fa\u56e0 \u7f16\u8f91 | April \u5ba1\u6838 | Barney<\/div>\n
\u5173\u952e\u8bcd | \u86cb\u767d\u7ec4\u00a0<\/div>\n
\u00a0<\/div>\n
01<\/div>\n
Matthias Mann\u6559\u6388<\/div>\n
\u5fb7\u56fdMax Plank<\/div>\n
\u751f\u7269\u5316\u5b66\u7814\u7a76\u6240\u6240\u957f<\/div>\n\n
\n
\"NUAhsbBqI8KjoZiafFQjIWvuDqcfOJw6L5YIiaarUSws2C0Ic0bQBDictLEUJOZeOtUvxfspO4AaUg2l8DSLNRk1g\"\/<\/figure><\/div>\n\n
\u5e08\u4ece\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\u5f97\u4e3bJohan Fenn\uff0c\u662f\u5fb7\u56fd\u88ab\u5f15\u7528\u6b21\u6570\u6700\u591a\u7684\u7814\u7a76\u5458\uff0ch\u6307\u6570\u8d85\u8fc7230\uff0c\u5f15\u7528\u6b21\u6570\u8d85\u8fc725\u4e07\u6b21\uff08google scholar\uff09\uff0c\u5728Nature Methods\uff0cCell\u7b49\u6742\u5fd7\u4e0a\u53d1\u8868\u4e86\u4e00\u7cfb\u5217\u8fd9\u4e00\u9886\u57df\u7684\u7814\u7a76\u6210\u679c\u3002\u4e0eJ\u00fcrgen Cox\u6559\u6388\u5408\u4f5c\u5f00\u53d1\u4e86MaxQuant\u8f6f\u4ef6\u2014\u2014\u4e00\u79cd\u7528\u4e8e\u86cb\u767d\u8d28\u7ec4\u5b66\u5b9a\u91cf\u5206\u6790\u8f6f\u4ef6\u5de5\u5177\uff0c\u53ef\u7528\u4e8e\u5206\u6790MS\u6240\u4ea7\u751f\u7684\u6570\u636e\u3002\u51ed\u501f\u7740\u4e25\u8c28\u800c\u5f3a\u5927\u7684\u529f\u80fd\uff0c\u4ee5\u53ca\u5bf9\u4f17\u591a\u5e73\u53f0\u7684\u5e7f\u6cdb\u652f\u6301\u6027\uff0cMaxQuant\u4e1a\u5df2\u6210\u4e3a\u86cb\u767d\u8d28\u7ec4\u5b66\u7814\u7a76\u4e2d\u6700\u5e38\u7528\u7684\u641c\u5e93\u8f6f\u4ef6\u5e73\u53f0\u4e4b\u4e00\u3002\u57fa\u4e8etimsTOF Pro\u76844D\u86cb\u767d\u7ec4\u5b66\u5e73\u53f0\u5c31\u662f\u7531\u5e03\u9c81\u514b\u548cMatthias Mann\u56e2\u961f\u5171\u540c\u5f00\u53d1\u3002Matthias Mann\u57282012\u5e74\u56e0\u5728\u86cb\u767d\u8d28\u7ec4\u5b66\u548c\u8d28\u8c31\u7814\u7a76\u9886\u57df\u7684\u7a81\u51fa\u8d21\u732e\u8363\u83b7\u5f97\u5f71\u54cd\u529b\u4ec5\u6b21\u4e8e\u8bfa\u8d1d\u5c14\u5956\u7684Louis-Jeantet\u533b\u5b66\u5956\u3002<\/div>\n
\u00a0<\/div>\n
02<\/div>\n
John R.Yates III<\/div>\n
\u6559\u6388<\/div>\n
\u7f8e\u56fdScripps\u7814\u7a76\u6240<\/div>\n\n
\n
\"NUAhsbBqI8KjoZiafFQjIWvuDqcfOJw6LBGyo9LxhOoCqVTu9e2GEZHuuT43rglV89LicBC4UmFibGRe2wcchcPCg\"\/<\/figure><\/div>\n\n
\u4e8e2011\u5e74\u8363\u767b\u201c2000-2010\u5e74\u5168\u7403\u9876\u5c16\u4e00\u767e\u4f4d\u5316\u5b66\u5bb6\u201d\u540d\u5355\uff0c\u4e5f\u662f2019\u5e74ASMS\u8d28\u8c31\u6770\u51fa\u8d21\u732e\u5956\u83b7\u5f97\u8005\u300220\u591a\u5e74\u6765\u81f4\u529b\u4e8e\u8d28\u8c31\u6280\u672f\u5728\u751f\u7269\u5b66\u4e2d\u7684\u5e94\u7528\u7814\u7a76\uff0c\u5f00\u53d1\u4e86SEQUEST\u8f6f\u4ef6\u548c\u591a\u7ef4\u86cb\u767d\u8d28\u9274\u5b9a\u6280\u672f\u5e73\u53f0\uff08MudPIT\uff09\u3002MudPIT\u7684\u51fa\u73b0\u76f4\u63a5\u63a8\u52a8\u4e86\u86cb\u767d\u8d28\u7ec4\u5b66\u7531\u57fa\u4e8e2D\u53cc\u5411\u7535\u6cf3\u8fc8\u5411\u57fa\u4e8e\u8d28\u8c31\u7684\u65f6\u4ee3\u3002<\/div>\n
\u00a0<\/div>\n
John R. Yates III\u6559\u6388\u4e0d\u4f46\u662f\u86cb\u767d\u8d28\u7ec4\u7814\u7a76\u7684\u6267\u725b\u8033\u8005\uff0c\u540c\u65f6\u4e5f\u4e00\u76f4\u5927\u529b\u652f\u6301\u6211\u56fd\u86cb\u767d\u8d28\u7ec4\u5b66\u884c\u4e1a\u7684\u53d1\u5c55\uff0c\u56fd\u5185\u8bf8\u591a\u8457\u540d\u7684\u86cb\u767d\u8d28\u7ec4\u5b66\u5bb6\u5982\u5317\u5927\u7684\u9ec4\u8d85\u5170\u6559\u6388\u3001NIBS\u7684\u8463\u68a6\u79cb\u7814\u7a76\u5458\u3001\u4e2d\u79d1\u9662\u6709\u673a\u6240\u7684\u5f20\u8000\u9633\u7814\u7a76\u5458\u548c\u534e\u4e1c\u5e08\u8303\u5927\u5b66\u7684\u5ed6\u9c81\u5251\u6559\u6388\u7b49\u90fd\u66fe\u5728\u4ed6\u7684\u8bfe\u9898\u7ec4\u4ece\u4e8b\u7814\u7a76\u5de5\u4f5c\u3002\u6b64\u5916\uff0cJohn R. Yates III\u6559\u6388\u4e5f\u7ecf\u5e38\u6765\u4e2d\u56fd\u8bbf\u95ee\uff0c\u5e76\u53c2\u52a0CNHUPO\u7b49\u5b66\u672f\u4f1a\u8bae\uff0c\u53ef\u4ee5\u8bf4\u662f\u4e2d\u56fd\u79d1\u5b66\u754c\u7684\u201c\u8001\u670b\u53cb\u201d\u4e86\u3002<\/div>\n
03<\/div>\n
\u9ec4\u8d85\u5170\u6559\u6388<\/div>\n
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\n
\"NUAhsbBqI8KjoZiafFQjIWvuDqcfOJw6Lmb3MIEw7QCqZD7U8OsibWHDdxOmT9YkjVHhzxuibpia4t1RoLHTNsIp0Q\"\/<\/figure><\/div>\n\n
\u201c\u6e2f\u5927\u5316\u5b66\u521d\u76f8\u89c1\uff0c\u4e00\u9047\u8d28\u8c31\u8bef\u7ec8\u8eab\u201d\uff0c\u81ea\u6b64\u9ec4\u8d85\u5170\u6559\u6388\u627e\u5230\u4e86\u4e00\u751f\u7684\u201c\u60c5\u4eba\u201d\u3002\u5979\u5e08\u4ece\u86cb\u767d\u8d28\u7ec4\u5b66\u754c\u7684\u6cf0\u6597John Yates\uff0c\u5728\u7531\u8d28\u8c31\u6280\u672f\u642d\u5efa\u7684\u4e16\u754c\u91cc\uff0c\u6e38\u5203\u6709\u4f59\u5730\u8fdb\u884c\u7740\u79d1\u7814\u548c\u65b9\u6cd5\u5f00\u53d1\u3002\u5728\u7f8e\u79d1\u7814\u5de5\u4f5c8\u5e74\uff0c\u76f4\u52302013\u5e74\u88ab\u4e2d\u79d1\u9662\u4eba\u624d\u62db\u8058\u56de\u56fd\u5efa\u8bbe\u56fd\u5bb6\u86cb\u767d\u8d28\u4e2d\u5fc3\uff0c\u624b\u628a\u624b\u5730\u5efa\u7acb\u4e86\u56fd\u5bb6\u86cb\u767d\u8d28\u79d1\u5b66\u4e2d\u5fc3\uff08\u4e0a\u6d77\uff09\u30022014\u5e74\u4ee5\u7b2c\u4e00\u7684\u7b54\u8fa9\u6210\u7ee9\u83b7\u5f97\u4e86\u201c\u4e2d\u79d1\u9662\u5f15\u8fdb\u6770\u51fa\u6280\u672f\u4eba\u624d\u201d\u7684\u8363\u8a89\u3002<\/div>\n
\u00a0<\/div>\n
\u5982\u4eca\u9ec4\u8d85\u5170\u6559\u6388\u62c5\u4efb\u5317\u4eac\u5927\u5b66\u533b\u5b66\u90e8\u7cbe\u51c6\u533b\u7597\u591a\u7ec4\u5b66\u7814\u7a76\u4e2d\u5fc3\u4e3b\u4efb\uff0c\u5317\u5927-\u6e05\u534e\u751f\u547d\u79d1\u5b66\u8054\u5408\u4e2d\u5fc3\u7814\u7a76\u5458\uff0c\u66fc\u5f7b\u65af\u7279\u5927\u5b66\u751f\u7269\u3001\u533b\u5b66\u4e0e\u5065\u5eb7\u5b66\u9662\u8363\u8a89\u6559\u6388\u3001\u300aBBA- PROTEINS PROTEOM\u300b\u671f\u520a\u526f\u4e3b\u7f16\uff0c\u4e2d\u56fd\u751f\u7269\u5316\u5b66\u4e0e\u5206\u5b50\u751f\u7269\u5b66\u4f1a\u86cb\u767d\u8d28\u4e13\u4e1a\u59d4\u5458\u4f1a\u59d4\u5458\uff0cCNHUPO\u4e13\u5bb6\u59d4\u5458\u4f1a\u59d4\u5458\u7b49\u804c\u3002\u957f\u671f\u81f4\u529b\u4e8e\u8d28\u8c31\u548c\u86cb\u767d\u8d28\u7ec4\u5b66\u524d\u6cbf\u65b0\u6280\u672f\u548c\u65b9\u6cd5\u7684\u7814\u7a76\u5f00\u53d1\uff0c\u5305\u62ec\u5355\u7ec6\u80de\u86cb\u767d\u8d28\u82af\u7247gOAD chip (3.0)\u3001\u7edd\u5bf9\u5b9a\u91cf\u86cb\u767d\u8d28\u7ec4\u65b0\u65b9\u6cd5\u3001\u4e34\u5e8a\u5927\u961f\u52174D-DIA\u9ad8\u901a\u91cf\u6280\u672f\u7b49\uff0c\u76ee\u524d\u5df2\u5728Cell\u3001Science\u3001Nature Method\u3001Nature Communications\u3001PLoS Biology\u3001PNAS\u3001Molecular Cell\u3001EMBO J\u7b49\u671f\u520a\u53d1\u8868\u8d85\u8fc780\u7bc7\u6587\u7ae0\u3002<\/div>\n
\u00a0<\/div>\n
schedule<\/div>\n
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6\u670824\u65e5<\/div>\n\n
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\"NUAhsbBqI8KjoZiafFQjIWvuDqcfOJw6LRHb4jV5rG9ib6piakGrS6bL6VNWBLvxbrgJqfiaVXl9SFTIlMokvdZCoA\"\/<\/figure><\/div>\n\n
sign up<\/div>\n
\u62a5\/\u540d\/\u65b9\/\u5f0f<\/div>\n\n
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\"NUAhsbBqI8KjoZiafFQjIWvuDqcfOJw6L5cx3dmKoyRUib68erMaA2ibBrVww1vR29QzJVGUQ6CcRde7CsaCNLG2w\"\/<\/figure><\/div>\n\n
\u626b\u63cf\u4e0a\u65b9\u4e8c\u7ef4\u7801<\/div>\n
\u5373\u53ef\u62a5\u540d\u672c\u6b21\u8bfe\u7a0b<\/div>\n
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[\u6ce8\uff1a\u4ee5\u4e0a\u5185\u5bb9\u53d7\u6743\u8f6c\u8f7d\u81ea\u201c\u5409\u51ef\u57fa\u56e0\u201d]<\/div>\n
\u00a0<\/div>\n
\u6269\u5c55\u9605\u8bfb<\/div>\n
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