\u300a2022\u57fa\u56e0\u884c\u4e1a\u84dd\u76ae\u4e66\u300b\u73b0\u5df2\u542f\u52a8\u5408\u4f5c\uff0c\u6b22\u8fce\u8054\u7cfb\u57fa\u56e0\u6167\uff08\u5fae\u4fe1\u53f7\uff1ajiyinhui_1\uff09\u8fdb\u884c\u6d3d\u8c08\u3002<\/div>\n
\u00a0<\/div>\n
\u6587\u7ae0 | \u57fa\u56e0\u6167\u7f16\u8f91 | Kathy\u00a0\u5ba1\u6838 | Mary<\/div>\n
\u5173\u952e\u8bcd | \u80bf\u7624\u57fa\u56e0\u53ca\u5206\u5b50\u68c0\u6d4b\u6280\u672f<\/div>\n\n
\n![\"RmFicvQBAkgDVIM1QFCj1wY42xic5JnILbNlLetdrFPhG7gYtP3oFwpxJuC9GeabdTqHU1qUCb4Ifdib2JIPzrQNg\"\/](\"https:\/\/www.geneclub.net.cn\/wp-content\/uploads\/images\/RmFicvQBAkgDVIM1QFCj1wY42xic5JnILbNlLetdrFPhG7gYtP3oFwpxJuC9GeabdTqHU1qUCb4Ifdib2JIPzrQNg.png\")
<\/figure><\/div>\n\n<\/div>\n
<\/figure><\/div>\n\n<\/div>\n<\/div>\n
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\u84dd\u76ae\u4e66\u8fde\u8f7d\u5185\u5bb9\u4ecb\u7ecd<\/div>\n
\u00a0<\/div>\n
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\u884c\u4e1a\u80cc\u666f\u548c\u6982\u51b5\uff1b<\/div>\n
\u80bf\u7624\u57fa\u56e0\u53ca\u5206\u5b50\u6807\u5fd7\u7269\u7b80\u4ecb\uff1b<\/div>\n
\u80bf\u7624\u57fa\u56e0\u53ca\u5206\u5b50\u68c0\u6d4b\u6280\u672f\u7b80\u4ecb\uff1b<\/div>\n
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\u80bf\u7624\u57fa\u56e0\u53ca\u5206\u5b50\u68c0\u6d4b\u884c\u4e1a\u7b80\u6790\uff1b<\/div>\n
\u672a\u6ee1\u8db3\u9700\u6c42\u53ca\u53d1\u5c55\u8d8b\u52bf\u3002<\/div>\n
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\n\n
\n![\"RmFicvQBAkgDVIM1QFCj1wY42xic5JnILbSdYCGq3z4OrGWUTAy924kYnxzGtDh7ic1alfzD1lqvuM5cf5rAOM5QQ\"\/](\"https:\/\/www.geneclub.net.cn\/wp-content\/uploads\/images\/RmFicvQBAkgDVIM1QFCj1wY42xic5JnILbSdYCGq3z4OrGWUTAy924kYnxzGtDh7ic1alfzD1lqvuM5cf5rAOM5QQ.png\")
<\/figure><\/div>\n\n<\/div>\n
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\u76f8\u6bd4\u4e8e\u4f20\u7edf\u7684\u7ec4\u7ec7\u6d3b\u68c0\uff0c\u6db2\u4f53\u6d3b\u68c0\u5177\u6709\u65e0\u521b\u6027\u3001\u654f\u611f\u6027\u3001\u52a8\u6001\u6027\u7b49\u7279\u8d28\uff0c\u5176\u51fa\u73b0\u6807\u5fd7\u7740\u4eba\u7c7b\u5728\u653b\u514b\u80bf\u7624\u764c\u75c7\u7684\u9053\u8def\u4e0a\u53c8\u524d\u8fdb\u4e86\u4e00\u5927\u6b65\u3002\u56e0\u6b64\uff0c\u5728\u4ecb\u7ecd\u5177\u4f53\u7684\u80bf\u7624\u57fa\u56e0\u53ca\u5206\u5b50\u68c0\u6d4b\u6280\u672f\u4e4b\u524d\uff0c\u5148\u5728\u6b64\u7b80\u8ff0\u6db2\u4f53\u6d3b\u68c0\u7684\u57fa\u672c\u60c5\u51b5\u3002<\/div>\n
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\u6db2\u4f53\u6d3b\u68c0<\/div>\n
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\u25a0\u00a0\u6db2\u4f53\u6d3b\u68c0\u662f\u4ec0\u4e48\uff1f<\/div>\n
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\u25a0\u00a0\u53d1\u5c55\u5386\u7a0b<\/div>\n
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\n\n
\n![\"RmFicvQBAkgDVIM1QFCj1wY42xic5JnILbn6SL0ibib4iafx0ZgFsicpMFgaczqaSCaGvmGcfpprV6vtzJGByiajPbRcg\"\/](\"https:\/\/www.geneclub.net.cn\/wp-content\/uploads\/images\/RmFicvQBAkgDVIM1QFCj1wY42xic5JnILbn6SL0ibib4iafx0ZgFsicpMFgaczqaSCaGvmGcfpprV6vtzJGByiajPbRcg.png\")
<\/figure><\/div>\n\n<\/div>\n
<\/figure><\/div>\n\n<\/div>\n<\/div>\n
\u25a0\u00a0\u8303\u7574<\/div>\n
\u6db2\u4f53\u6d3b\u68c0\u7684\u91c7\u6837\u7c7b\u578b\u5305\u62ec\uff1a\u8840\u6db2\uff08\u5916\u5468\u8840\uff09\u3001\u553e\u6db2\u3001\u5c3f\u6db2\u3001\u8111\u810a\u6db2\u3001\u80f8\u8154\u79ef\u6db2\u7b49\uff1b<\/div>\n
\u6db2\u4f53\u6d3b\u68c0\u5206\u6790\u7684\u5206\u5b50\u6807\u5fd7\u7269\u5305\u62ec\uff1actDNA\u3001CTC\u3001\u5916\u6ccc\u4f53\uff08\u5916\u56ca\u6ce1\u7684\u4e00\u79cd\uff09\u3001\u80bf\u7624\u57f9\u517b\u7684\u8840\u5c0f\u677f\u7b49\u3002\u6839\u636e\u7f8e\u56fd\u75c5\u7406\u534f\u4f1a\u5b98\u7f51\uff0c\u6db2\u4f53\u6d3b\u68c0\u76ee\u524d\u7684\u91cd\u70b9\u662f ctDNA\uff0c\u5176\u4ed6\u5305\u62ec RNA\u3001CTC\u3001\u7ec6\u80de\u5916\u56ca\u6ce1\uff08EV\uff09\u548c\u80bf\u7624\u57f9\u517b\u7684\u8840\u5c0f\u677f\uff08TEP\uff09\u3002<\/div>\n
\u25a0\u5e94\u7528<\/div>\n
\u6db2\u4f53\u6d3b\u68c0\u4e3b\u8981\u57fa\u4e8e\u80bf\u7624\u91ca\u653e\u7684\u7ec6\u80de\u53ca\u6838\u9178\uff08DNA \u548c RNA\uff09\u6765\u8bc4\u4f30\u6cbb\u7597\u53cd\u5e94\uff08\u9884\u540e\u76d1\u6d4b\u3001\u6b8b\u7559\u75be\u75c5\u68c0\u6d4b\uff09\u3001\u9884\u6d4b\u80bf\u7624\u590d\u53d1\u98ce\u9669\u3001\u8ffd\u8e2a\u4e0e\u8010\u836f\u6027\u76f8\u5173\u7684\u7a81\u53d8\u3001\u53ef\u9776\u5411\u7684\u9a71\u52a8\u7a81\u53d8\u7814\u7a76\u548c\u4e34\u5e8a\u8bd5\u9a8c\u6ce8\u518c\u7b49\uff0c\u53ef\u4ee5\u5e94\u7528\u4e8e\u5e94\u7528\u4e8e\u80bf\u7624\u5206\u5b50\u5206\u578b\u3001\u80bf\u7624\u6f14\u53d8\u53ca\u9884\u540e\u76d1\u6d4b\u7b49\u573a\u666f\u3002<\/div>\n
\u25a0\u00a0\u4f18\u52bf<\/div>\n
\u5728\u4e34\u5e8a\u5e94\u7528\u4e2d\uff0c\u7ec4\u7ec7\u6d3b\u68c0\u901a\u5e38\u662f\u80bf\u7624\u8bca\u65ad\u548c\u6cbb\u7597\u7684\u91d1\u6807\u51c6\uff0c\u4f46\u5b58\u5728\u4e24\u65b9\u9762\u5f0a\u7aef\uff1a\u4e00\u662f\u4fb5\u5165\u6027\u610f\u5473\u7740\u6837\u672c\u6709\u65f6\u65e0\u6cd5\u53d6\u5f97\uff0c\u9700\u8981\u8003\u8651\u80bf\u7624\u7684\u4f4d\u7f6e\u548c\u60a3\u8005\u7684\u72b6\u6001\uff1b\u4e8c\u662f\u7ec4\u7ec7\u6837\u672c\u7684\u542b\u91cf\u4f4e\u7b49\u65e0\u6cd5\u6ee1\u8db3 NGS \u7b49\u68c0\u6d4b\u7684\u8981\u6c42\u3002<\/div>\n
\u76f8\u5bf9\u7ec4\u7ec7\u6d3b\u68c0\uff0c\u6db2\u4f53\u6d3b\u68c0\u7684\u6574\u4f53\u4f18\u52bf\u4e3b\u8981\u5728\u4e8e\u83b7\u5f97\u4fb5\u5165\u6027\u8981\u5c0f\u5f97\u591a\uff0c\u5141\u8bb8\u9891\u7e41\u3001\u8fde\u7eed\u7684\u91c7\u6837\uff0c\u4ece\u800c\u6301\u7eed\u8ffd\u8e2a\u80bf\u7624\u53d1\u5c55\u53ca\u9884\u540e\u53cd\u5e94\uff1b\u53e6\u5916\u53ef\u4ee5\u5728\u65e0\u6cd5\u8fdb\u884c\u7ec4\u7ec7\u6d3b\u68c0\u4e0b\u8fdb\u884c\u6db2\u4f53\u6d3b\u68c0\u3002<\/div>\n
\u25a0\u00a0\u524d\u666f<\/div>\n
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\u57fa\u4e8e\u5df2\u83b7\u6279\u4ea7\u54c1\uff0c\u6db2\u4f53\u6d3b\u68c0\u4e5f\u5927\u5927\u52a0\u901f\u4e86\u65b0\u836f\u7814\u53d1\u7684\u9776\u70b9\u53d1\u73b0\u3001\u4e34\u5e8a\u8bd5\u9a8c\u5165\u7ec4\u4ee5\u53ca\u4f34\u968f\u8bca\u65ad\u7684\u53d1\u5c55\uff0c\u5728\u9884\u540e\u68c0\u6d4b\u3001\u590d\u53d1\u8bc4\u4f30\u7b49\u65b9\u9762\u6709\u7740\u5de8\u5927\u7684\u524d\u666f\uff0c\u6838\u5fc3\u6311\u6218\u662f\u63d0\u9ad8\u7075\u654f\u5ea6\u3002\u8be5\u9886\u57df\u7684\u4e0b\u4e00\u4e2a\u6311\u6218\u548c\u673a\u4f1a\u662f\u5c06\u6db2\u4f53\u6d3b\u68c0\u7eb3\u5165\u5e38\u89c4\u764c\u75c7\u7b5b\u67e5\u8ba1\u5212\uff0c\u4ee5\u4fc3\u8fdb\u764c\u75c7\u65e9\u671f\u8bca\u65ad\u3002<\/div>\n
<\/div>\n
\u6db2\u4f53\u6d3b\u68c0\u5305\u62ec\u591a\u79cd\u6280\u672f\uff0c\u4ee5\u4e0b\u5c06\u4ecb\u7ecd\u5176\u4e2d\u6700\u5177\u4ee3\u8868\u6027\u7684\u51e0\u79cd\u3002<\/div>\n
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\u8367\u5149\u5b9a\u91cfPCR\uff08qPCR\uff09\u53ca\u6570\u5b57PCR\uff08dPCR\uff09<\/div>\n
\u25a0\u00a0\u5b9a\u4e49\u53ca\u610f\u4e49<\/div>\n
PCR\uff08Polymerase Chain Reactio\uff0c\u805a\u5408\u9176\u94fe\u5f0f\u53cd\u5e94\uff09\u662f\u5728\u4f53\u5916\u6a21\u62df DNA \u590d\u5236\u7684\u8fc7\u7a0b\uff0c\u5feb\u901f\u6269\u589e\u7279\u5b9a\u57fa\u56e0\u6216 DNA \u5e8f\u5217\uff0c\u53c8\u79f0\u4e3a\u57fa\u56e0\u7684\u4f53\u5916\u6269\u589e\u6cd5\u3002PCR \u662f\u751f\u7269\u82af\u7247\u3001\u6d4b\u5e8f\u7b49\u5206\u5b50\u68c0\u6d4b\u6280\u672f\u4e2d\u7684\u57fa\u7840\u6280\u672f\uff0c\u4e5f\u662f\u4e00\u9879\u72ec\u7acb\u7684\u68c0\u6d4b\u6280\u672f\uff0c\u5bf9\u533b\u5b66\u8bca\u65ad\u7b49\u9886\u57df\u662f\u9769\u65b0\u5f0f\u63a8\u52a8\u3002<\/div>\n
\u00a0<\/div>\n
\u25a0\u00a0\u539f\u7406\u53ca\u6b65\u9aa4<\/div>\n
<\/div>\n
\u5b8c\u6210 PCR \u8fc7\u7a0b\u7684\u57fa\u672c\u8981\u7d20\u5305\u62ec\uff1a<\/div>\n
\u25cf DNA \u6a21\u677f\uff1a\u5305\u542b\u76ee\u6807\u5e8f\u5217\u7684\u6837\u672c DNA<\/div>\n
\u25cf DNA \u805a\u5408\u9176\uff1a\u5408\u6210\u4e0e\u76ee\u6807\u5e8f\u5217\u4e92\u8865\u7684\u65b0 DNA \u94fe\u7684\u9176\uff0c\u5982TaqDNA \u805a\u5408\u9176<\/div>\n
\u25cf\u00a0\u6838\u82f7\u9178\uff1adNTP\uff08A\/T\/C\/G\uff09 \u6216\u8131\u6c27\u6838\u82f7\u9178\u4e09\u78f7\u9178\uff0c\u662f\u6784\u5efa\u65b0 DNA \u94fe\u7684\u5143\u7d20<\/div>\n
\u25cf\u00a0\u9006\u8f6c\u5f55\u9176\uff1a\u5728\u9006\u8f6c\u5f55 PCR\u4e2d\u9700\u7528\u9006\u8f6c\u5f55\u9176\u5c06\u6837\u54c1 RNA \u8f6c\u5316\u4e3a cDNA<\/div>\n
\u00a0<\/div>\n
\n\n
\n![\"RmFicvQBAkgDVIM1QFCj1wY42xic5JnILbkjbghfJiayhSfL7pvblZFvhXtcmNIKT02ibpKpEw2ufkUrhSUwZ7lyicQ\"\/](\"https:\/\/www.geneclub.net.cn\/wp-content\/uploads\/images\/RmFicvQBAkgDVIM1QFCj1wY42xic5JnILbkjbghfJiayhSfL7pvblZFvhXtcmNIKT02ibpKpEw2ufkUrhSUwZ7lyicQ.png\")
<\/figure><\/div>\n\n<\/div>\n
<\/figure><\/div>\n\n<\/div>\n<\/div>\n
\u00a0<\/div>\n
<\/div>\n
\u25a0\u00a0\u7c7b\u578b<\/div>\n
\u00a0<\/div>\n
\n\n
\n![\"RmFicvQBAkgDVIM1QFCj1wY42xic5JnILb5CT7kEjwtBlibbtNpaGGCFMLMLT9QXb497Lb0mAhcYpG0JBnps4atVQ\"\/](\"https:\/\/www.geneclub.net.cn\/wp-content\/uploads\/images\/RmFicvQBAkgDVIM1QFCj1wY42xic5JnILb5CT7kEjwtBlibbtNpaGGCFMLMLT9QXb497Lb0mAhcYpG0JBnps4atVQ.png\")
<\/figure><\/div>\n\n<\/div>\n
<\/figure><\/div>\n\n<\/div>\n<\/div>\n
\u00a0<\/div>\n
\u25a0\u00a0\u5b9e\u9645\u5e94\u7528<\/div>\n
<\/div>\n
\u6b64\u7c7b\u6280\u672f\u7684\u5b9e\u9645\u5e94\u7528\u573a\u666f\u6781\u591a\uff0c\u4f8b\u5982\uff0c\u5bf9\u4e8e\u7c7b\u4f3c\u4e8e\u6162\u6027\u9aa8\u9ad3\u6027\u767d\u8840\u75c5\u7b49\u8840\u6db2\u7624\uff0cqPCR \u6280\u672f\u53ef\u4ee5\u901a\u8fc7\u68c0\u6d4b\u539f\u764c\u57fa\u56e0\u6613\u4f4d\u5bfc\u81f4\u7684 BCR\/ABL \u878d\u5408\u57fa\u56e0\u7684\u8868\u8fbe\uff0c\u6765\u786e\u5b9a\u5fae\u91cf\u6b8b\u4f59\u80bf\u7624\u7ec6\u80de\u7684\u6570\u91cf\uff0c\u8fdb\u884c\u6cbb\u7597\u6548\u679c\u8bc4\u4f30\u548c\u590d\u53d1\u9884\u6d4b\u3002<\/div>\n
<\/div>\n
\u00a0<\/div>\n
\u901a\u8fc7\u4f7f\u7528\u5e26\u6709\u7a81\u53d8\u7279\u5f02\u6027\u63a2\u9488\u7684 dPCR\uff0c\u53ef\u4ee5\u5728 cfDNA \u4e2d\u7684\u67d0\u4e9b\u7a81\u53d8\u5f00\u59cb\u51fa\u73b0\u5728\u4f53\u6db2\u4e2d\u65f6\u5c31\u68c0\u6d4b\u5230\u5b83\u4eec\u3002\u6b64\u5916\uff0cdPCR \u7684\u9ad8\u7cbe\u5ea6\u548c\u7075\u654f\u5ea6\u53ef\u7528\u4e8e\u76d1\u6d4b\u764c\u75c7\u8fdb\u5c55\u548c\u65e9\u671f\u68c0\u6d4b\u590d\u53d1\u3002<\/div>\n
\u00a0<\/div>\n
dPCR \u9664\u4e86\u53ef\u4ee5\u68c0\u6d4b\u70b9\u7a81\u53d8\uff0c\u8fd8\u53ef\u7528\u4e8e\u68c0\u6d4b CNV\u3002\u6b64\u5916\uff0c\u7814\u7a76\u663e\u793a\uff0c dPCR \u68c0\u6d4b HPV \u4e2d mRNA\uff0c\u5bf9\u4e8e\u7ec4\u7ec7\u5b66\u4e0a\u65e0\u80bf\u7624\u7684\u6dcb\u5df4\u7ed3\u4e2d\u7684\u9690\u533f\u6027\u80bf\u7624\u7ec6\u80de\u8fdb\u884c\u4e34\u5e8a\u8bc4\u4f30\u6709\u8f83\u5927\u7684\u5e94\u7528\u6f5c\u529b\u3002<\/div>\n
<\/div>\n
<\/div>\n
\u4e0b\u4e00\u4ee3\u6d4b\u5e8f\uff08NGS\uff09<\/div>\n
\u25a0\u00a0\u5b9a\u4e49<\/div>\n
\u4e0b\u4e00\u4ee3\u6d4b\u5e8f\uff08Next Generation Sequencing\uff0cNGS\uff09\u4e5f\u88ab\u79f0\u4e3a\u5927\u89c4\u6a21\u5e73\u884c\u6d4b\u5e8f\uff08MPS\uff09\u6216\u9ad8\u901a\u91cf\u6d4b\u5e8f\uff08HTS\uff09\uff0c\u662f\u4e00\u79cd\u5927\u89c4\u6a21\u3001\u5e76\u884c\u3001\u9ad8\u901a\u91cf\u6d4b\u5e8f\u6280\u672f\uff0c\u4ee5\u53ef\u6269\u5c55\u6027\u3001\u5feb\u901f\u548c\u53ef\u53ca\u6027\u7b49\u7279\u70b9\uff0c\u6765\u786e\u5b9a\u57fa\u56e0\u7ec4\u6216\u90e8\u5206\u6838\u9178\uff08DNA\/RNA\uff09\u5e8f\u5217\u7684\u6392\u5217\u987a\u5e8f\uff0c\u8fdb\u800c\u5206\u6790\u5bf9\u5e94\u8c03\u63a7\u3001\u7f16\u7801\u86cb\u767d\u8d28\u7b49\u751f\u7269\u4f53\u7279\u5f81\u3002\u00a0<\/div>\n
\u00a0<\/div>\n
<\/div>\n
\n\n
\n![\"RmFicvQBAkgDVIM1QFCj1wY42xic5JnILbq02ic8futrlxVjzJ0MzGwECNkTjmV3HnDElewGVWwJ9OE88Yhq6sZXA\"\/](\"https:\/\/www.geneclub.net.cn\/wp-content\/uploads\/images\/RmFicvQBAkgDVIM1QFCj1wY42xic5JnILbq02ic8futrlxVjzJ0MzGwECNkTjmV3HnDElewGVWwJ9OE88Yhq6sZXA.png\")
<\/figure><\/div>\n\n
<\/figure><\/div>\n\n\u25a0\u00a0\u7c7b\u578b<\/div>\n
\u4ece\u5206\u7c7b\u4e0a\uff0cNGS \u5305\u62ec\u77ed\u8bfb\u957f\u6d4b\u5e8f\u53ca\u957f\u8bfb\u957f\u6d4b\u5e8f\uff0c\u77ed\u8bfb\u957f\u6d4b\u5e8f\u5728\u51c6\u786e\u7387\u53ca\u6210\u672c\u4e0a\u76f8\u5bf9\u6210\u719f\uff0c\u5e7f\u6cdb\u5e94\u7528\u4e8e\u57fa\u7840\u7814\u7a76\u53ca\u4e34\u5e8a\u7b49\u5e94\u7528\uff0c\u4f46\u56e0\u4e3a\u8bfb\u957f\u77ed\u5bf9\u4e8e\u5927\u7247\u6bb5\u7ed3\u6784\u53d8\u5f02\u53ca\u590d\u6742\u57fa\u56e0\u7ec4\u7ec4\u88c5\u7b49\u65b9\u9762\u6709\u5c40\u9650\uff1b<\/div>\n
\u00a0<\/div>\n
\u957f\u8bfb\u957f\u6d4b\u5e8f\u76ee\u524d\u4e3b\u8981\u5e94\u7528\u4e8e\u57fa\u7840\u7814\u7a76\uff0c\u4e34\u5e8a\u5e94\u7528\u4ecd\u5728\u63a2\u7d22\u4e2d\uff0c\u51c6\u786e\u7387\u53ca\u6210\u672c\u4ecd\u9700\u4f18\u5316\uff0c\u4f46\u5f88\u597d\u5f25\u8865\u4e86 NGS \u5728\u957f\u7247\u6bb5\u68c0\u6d4b\u65b9\u9762\u7684\u5c40\u9650\u3002\u57fa\u56e0\u6167\u8ba4\u4e3a\uff0c\u5728 5-10 \u5e74\u5185\uff0c\u77ed\u8bfb\u957f\u548c\u957f\u8bfb\u957f\u5c06\u5728\u5e02\u573a\u4e0a\u4e92\u8865\u5171\u5b58\u3002\u672a\u6765\u968f\u7740\u6280\u672f\u8fed\u4ee3\uff0c\u6781\u53ef\u80fd\u4f1a\u51fa\u73b0\u96c6\u6210 Sanger \u6d4b\u5e8f\u3001\u77ed\u8bfb\u957f\u548c\u957f\u8bfb\u957f\u4f18\u52bf\u7684\u6280\u672f\u53ca\u5de5\u5177\u51fa\u73b0\u3002<\/div>\n
\u00a0<\/div>\n
\u25a0\u00a0\u4f18\u52bf<\/div>\n
NGS \u5728\u7eb3\u7c73\u7ea7\u7a7a\u95f4\u5185\u8fdb\u884c PCR \u53cd\u5e94\u6765\u6269\u589e DNA\uff08ONT \u7b49\u4e0d\u9700\u7528\u5230 PCR\uff09\uff0c\u57fa\u4e8e\u5927\u89c4\u6a21\u5e73\u884c\u7684\u53cd\u5e94\u4ea7\u751f\u5de8\u5927\u6570\u636e\u91cf\u3002\u5355\u4f4d\u53cd\u5e94\u4ea7\u751f\u7684\u6570\u636e\u91cf\u9010\u5e74\u63d0\u9ad8\uff0c\u6210\u672c\u8d85\u6469\u5c14\u5b9a\u5f8b\u4e0b\u964d\uff0c\u76ee\u524d\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\uff0830x\uff09\u6210\u672c\u4f4e\u4e8e 500 \u7f8e\u5143\u3002\u6781\u5927\u63a8\u52a8\u4e86\u4eba\u53e3\u7ea7\u5927\u89c4\u6a21\u57fa\u56e0\u6d4b\u5e8f\u57fa\u7840\u7814\u7a76\uff0c\u5bf9\u4e8e\u5c06\u57fa\u56e0\u6280\u672f\u5e94\u7528\u5230\u4e34\u5e8a\u8f85\u52a9\u8bca\u65ad\u53ca\u4f34\u968f\u8bca\u65ad\u3001\u65b0\u836f\u7814\u53d1\u3001\u75c5\u539f\u6eaf\u6e90\u7b49\u4f5c\u51fa\u4e86\u91cd\u8981\u8d21\u732e\u3002<\/div>\n
\u00a0<\/div>\n
\u6b64\u5916\uff0c\u968f\u7740 PacBio \u7684 SMRT \u6280\u672f\u4f18\u5316\uff0c\u590d\u6742\u7269\u79cd\u4ee5\u53ca\u5fae\u751f\u7269\u7684 de novo \u7ec4\u88c5\u5f97\u4ee5\u7a81\u7834\uff0c\u63a8\u52a8\u751f\u7269\u591a\u6837\u6027\u7814\u7a76\uff1bONT \u7684 Nanopore \u6d4b\u5e8f\u6280\u672f\u4e0d\u4f9d\u8d56 PCR\uff0c\u6700\u957f\u8bfb\u957f\u8d85\u8fc7 4 Mb\uff0c\u7ed3\u6784\u53d8\u5f02\u68c0\u6d4b\u65e5\u8d8b\u6210\u719f\uff1b\u5176\u4fbf\u643a\u5f0f\u8bbe\u5907\u4e5f\u8ba9\u6d4b\u5e8f\u4ece\u5b9e\u9a8c\u5ba4\u5230\u91ce\u5916\uff0c\u4ece\u5730\u7403\u5230\u592a\u7a7a\u5c55\u5f00\u66f4\u5e7f\u9614\u7684\u5e94\u7528\u573a\u666f\u3002<\/div>\n
\u00a0<\/div>\n
\u25a0\u00a0\u5c40\u9650<\/div>\n
\u5c3d\u7ba1\u4f18\u52bf\u5f88\u5927\uff0cNGS \u4ecd\u5b58\u5728\u4e0d\u5c11\u5c40\u9650\u6027\uff0c\u4f8b\u5982\uff1a<\/div>\n
\u25cf\u00a0\u5728\u764c\u75c7\u6db2\u4f53\u6d3b\u68c0\u4ecd\u9700\u63d0\u9ad8\u7075\u654f\u5ea6\uff0c\u7279\u522b\u662f\u4f4e\u6d53\u5ea6\u6837\u672c\u548c\u4f4e\u9891\u7a81\u53d8\uff1b<\/div>\n
\u25cf\u00a0\u6210\u672c\u548c\u5468\u671f\u4ecd\u9700\u4f18\u5316\uff1b<\/div>\n
\u25cf\u00a0\u77e5\u8bc6\u5e93\u7684\u532e\u4e4f\u4f7f\u5f97\u6d4b\u5e8f\u540e\u5e8f\u5217\u96be\u4ee5\u89e3\u8bfb\uff0c\u5bf9\u5e94\u91c7\u53d6\u7684\u4e34\u5e8a\u57fa\u56e0 Panel \u4ea7\u54c1\u8bbe\u8ba1\u4ecd\u4e0d\u591f\u7075\u6d3b\u548c\u53ef\u6269\u5c55\u3002<\/div>\n
\u00a0<\/div>\n
\u25a0\u00a0\u5b9e\u9645\u5e94\u7528<\/div>\n
NGS \u5e26\u6765\u5927\u91cf\u7269\u79cd\u53ca\u5927\u89c4\u6a21\u6837\u672c\u7684\u57fa\u56e0\u7ec4\u89e3\u6790\uff0c\u8fdb\u800c\u4e0e IT \u6280\u672f\uff08\u751f\u7269\u4fe1\u606f\u3001\u751f\u7269\u8ba1\u7b97\u3001\u673a\u5668\u5b66\u4e60\uff09\u7b49\u7ed3\u5408\uff0c\u9769\u65b0\u5bf9\u764c\u75c7\u53d1\u5c55\u548c\u8fdb\u5c55\u7684\u57fa\u7840\u7406\u89e3\u3001\u4e34\u5e8a\u5e94\u7528\u4ee5\u53ca\u6cbb\u7597\u3002\u76ee\u524d\uff0cNGS\u5df2\u5e7f\u6cdb\u5e94\u7528\u4e8e\u533b\u5b66\u3001\u516c\u5171\u536b\u751f\u3001\u519c\u4e1a\u3001\u6cd5\u533b\u3001\u751f\u7269\u591a\u6837\u6027\u7b49\u65b9\u9762\u7684\u57fa\u7840\u7814\u7a76\u3001\u8f85\u52a9\u51b3\u7b56\u53ca\u5206\u6790\u3002<\/div>\n
\u00a0<\/div>\n
\u514d\u75ab\u7ec4\u5316\uff08IHC\uff09<\/div>\n
\u25a0\u00a0\u5b9a\u4e49\u53ca\u539f\u7406<\/div>\n
\u514d\u75ab\u7ec4\u7ec7\u5316\u5b66\uff08immunohistochemistry\uff0cIHC\uff09\u7b80\u79f0\u514d\u75ab\u7ec4\u5316\uff0c\u901a\u8fc7\u6807\u8bb0\u7684\u7279\u5f02\u6027\u6297\u4f53\u5728\u7ec4\u7ec7\u7ec6\u80de\u539f\u4f4d\u7ed3\u5408\u76ee\u6807\u6297\u539f\uff0c\u901a\u8fc7\u6297\u539f\u6297\u4f53\u53cd\u5e94\u548c\u7ec4\u7ec7\u5316\u5b66\u7684\u5448\u8272\u53cd\u5e94\uff0c\u53ef\u89c6\u5316\u76f8\u5e94\u6297\u539f\u5728\u7ec6\u80de\u5185\u53ca\u5176\u9002\u5f53\u7684\u7ec4\u7ec7\u5b66\u80cc\u666f\u7684\u9ad8\u5206\u8fa8\u7387\u5206\u5e03\u548c\u5b9a\u4f4d\u3002<\/div>\n
\u00a0<\/div>\n
\u25a0\u00a0\u6b65\u9aa4<\/div>\n
\u901a\u8fc7\u5207\u7247\u673a\u5236\u4f5c\u75c5\u7406\u5207\u7247\uff0c\u4e0e\u6297\u4f53\u4e00\u8d77\u57f9\u517b\u3002\u6297\u4f53\u7ed3\u5408\u4f4d\u70b9\u4e0e\u6807\u8bb0\u7269\uff08\u5982\u8367\u5149\u67d3\u6599\u3001\u9176\u3001\u653e\u5c04\u6027\u5143\u7d20\u6216\u80f6\u4f53\u91d1\uff09\u76f8\u8fde\uff0c\u5728\u663e\u5fae\u955c\u4e0b\u53ef\u89c6\u5316\u3002IHC \u53cd\u5e94\u53ef\u4ee5\u901a\u8fc7\u653e\u5c04\u81ea\u663e\u5f71\u6765\u89c2\u5bdf\uff0c\u6838\u5fc3\u662f\u901a\u8fc7\u5bf9\u7ec6\u80de\u6216\u7ec4\u7ec7\u9020\u6210\u6700\u5c11\u7684\u635f\u4f24\u6765\u5bf9\u5927\u90e8\u5206 IHC \u8fdb\u884c\u67d3\u8272\uff0c\u5e76\u901a\u8fc7\u4f7f\u7528\u6700\u5c11\u7684\u6297\u4f53\uff0c\u5728\u80bf\u7624\u5206\u578b\u548c\u80bf\u7624\u6807\u5fd7\u7269\u4e0a\u627e\u5230\u4e00\u6761\u8def\u3002<\/div>\n
\u00a0<\/div>\n
\u25a0\u00a0\u4f18\u52bf\u4e0e\u5b9e\u9645\u5e94\u7528<\/div>\n
\u7531\u4e8e IHC \u6d89\u53ca\u7279\u5b9a\u7684\u6297\u539f – \u6297\u4f53\u53cd\u5e94\uff0c\u4e14\u80fd\u591f\u5c06\u6297\u539f\u7684\u5b58\u5728\u4e0e\u5176\u5728\u7ec4\u7ec7\u6216\u7ec6\u80de\u4e2d\u7684\u4f4d\u7f6e\u76f8\u5173\u8054\uff0c\u56e0\u6b64\u5b83\u6bd4\u4f20\u7edf\u86cb\u767d\u8d28\u68c0\u6d4b\u65b9\u6cd5\u5177\u6709\u660e\u663e\u4f18\u52bf\u3002\u76ee\u524dIHC\u662f\u5728\u4e34\u5e8a\u4e0a\u5e94\u7528\u6700\u4e3a\u5e7f\u6cdb\u7684\u75c5\u7406\u8bca\u65ad\u6280\u672f\uff0c\u5e7f\u6cdb\u5e94\u7528\u4e8e\u8bb8\u591a\u533b\u5b66\u7814\u7a76\u5b9e\u9a8c\u5ba4\u548c\u4e34\u5e8a\u8bca\u65ad\uff0c\u7279\u522b\u662f\u7ec6\u80de\u529f\u80fd\u7814\u7a76\u548c\u5206\u5b50\u75c5\u7406\u68c0\u6d4b\u3002\u514d\u75ab\u7ec4\u5316\u68c0\u6d4b\u53ef\u4ee5\u8bca\u65ad\u6765\u6e90\u4e0d\u660e\u3001\u539f\u53d1\u7076\u548c\u672a\u77e5\u539f\u53d1\u80bf\u7624\u8f6c\u79fb\u7684\u80bf\u7624\uff0c\u8fd8\u53ef\u4ee5\u9884\u6d4b\u4e73\u817a\u764c\u548c\u524d\u5217\u817a\u764c\u7684\u6cbb\u7597\u53cd\u5e94\uff0c\u5e76\u8fdb\u884c\u80bf\u7624\u7684\u75c5\u7406\u5b66\u5206\u578b\uff0c\u5728\u80bf\u7624\u57fa\u7840\u7814\u7a76\u548c\u4e34\u5e8a\u5e94\u7528\u4e0a\u8d77\u5230\u91cd\u8981\u4f5c\u7528\u3002<\/div>\n
\u00a0<\/div>\n
\u8d28\u8c31\u5206\u6790\uff08MS\uff09<\/div>\n
\u25a0\u00a0\u5b9a\u4e49\u53ca\u539f\u7406<\/div>\n
\u8d28\u8c31\uff0c\u5373\u662f\u4f7f\u6837\u672c\u4e2d\u5404\u7ec4\u5206\u7535\u79bb\uff0c\u751f\u6210\u4e0d\u540c\u8377\u8d28\u6bd4\u7684\u79bb\u5b50\uff0c\u7ecf\u7535\u573a\u4f5c\u7528\u5f62\u6210\u79bb\u5b50\u675f\u8fdb\u5165\u8d28\u91cf\u5206\u6790\u5668\uff0c\u4e0d\u540c\u8d28\u8377\u6bd4\u7684\u79bb\u5b50\u805a\u7126\u5728\u4e0d\u540c\u7684\u70b9\u4e0a\uff0c\u5f97\u5230\u8d28\u8c31\u56fe\uff0c\u4ece\u800c\u786e\u5b9a\u5176\u8d28\u91cf\u8fdb\u800c\u8fdb\u884c\u7ec4\u5206\u7684\u5b9a\u6027\u5206\u6790 ; \u8c31\u5cf0\u5f3a\u5ea6\u4e0e\u5316\u5408\u7269\u542b\u91cf\u6709\u5173\uff0c\u636e\u6b64\u8fdb\u884c\u5b9a\u91cf\u5206\u6790\u3002<\/div>\n
\u00a0<\/div>\n
\u25a0\u00a0\u7c7b\u578b<\/div>\n
\u8d28\u8c31\u5206\u4e3a\u591a\u79cd\u7c7b\u578b\uff0c\u5305\u62ec\u6c14\u76f8\u8272\u8c31\u8d28\u8c31\u8054\u7528\u4eea\uff08GC-MS\uff09\u3001\u6db2\u76f8\u8272\u8c31\u548c\u4e32\u8054\u8d28\u8c31\u8054\u7528\u6280\u672f\uff08LC-MS\/MS\uff09\u3001\u4e32\u8054\u8d28\u8c31\uff08MS-MS\uff09\u3001\u57fa\u8d28\u8f85\u52a9\u6fc0\u5149\u89e3\u5438\u98de\u884c\u65f6\u95f4\u8d28\u8c31\u4eea\uff08MALDI-TOF MS\uff09\u7b49\u3002<\/div>\n
\u00a0<\/div>\n
\u25a0\u00a0\u4f18\u52bf\u53ca\u5b9e\u9645\u5e94\u7528<\/div>\n
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