{"id":8827,"date":"2021-11-08T15:27:40","date_gmt":"2021-11-08T07:27:40","guid":{"rendered":"http:\/\/mp.weixin.qq.com\/s?__biz=MzI3NjUzOTM1Mg==&mid=100021317&idx=1&sn=2da658bf8328b737ffe4e046f8dcdf8c&chksm=6b7166f25c06efe4f70dbd8a2e94d9fcaea80cfe6524ca7b1c7dbf592bc0d082746174397900#rd"},"modified":"2026-04-07T17:15:42","modified_gmt":"2026-04-07T09:15:42","slug":"10256102","status":"publish","type":"post","link":"https:\/\/www.geneclub.net.cn\/index.php\/2021\/11\/08\/10256102\/","title":{"rendered":"\u8d5b\u9ed8\u98de\u5168\u81ea\u52a8\u4e00\u4f53\u5316\u6570\u5b57PCR\u7cfb\u7edf\u91cd\u78c5\u4e0a\u5e02"},"content":{"rendered":"
\n
\"Of3G0JtnqHusqZsa8nj3weB4nKxpHqY11CTKQaVCl9jCu5omKIeEwjyPyC3O6mZGWv1XJwKkwhrDwF5K7v9LZw\"\/<\/figure><\/div>\n\n
\u81ea1996\u5e74Applied Biosystems\u516c\u53f8\u63a8\u51fa\u9996\u6b3e\u8367\u5149\u5b9a\u91cfPCR\u4eea\u4ee5\u6765\uff0c\u521b\u65b0\u4ece\u672a\u6b62\u6b65\uff0c\u6280\u672f\u9769\u65b0\u4e0e\u7ecf\u5178\u4f20\u627f\u76f8\u5f97\u76ca\u5f70\u3002<\/div>\n
<\/div>\n\n
\n
\"Of3G0JtnqHusqZsa8nj3weB4nKxpHqY1bhAXXRWhbcfgicTYVwbdFjYEFyzZeKhRLGDJojgI0fSqQY2aBukcUpg\"\/<\/figure><\/div>\n\n
\n
\"Of3G0JtnqHusqZsa8nj3weB4nKxpHqY1VIgoap8ydWiaoBGniccf7TRxMia4EBfWreJ1djGFGeOg5LgbibDwaGwRBA\"\/<\/figure><\/div>\n\n
\n
\"Of3G0JtnqHusqZsa8nj3weB4nKxpHqY1uAR9cEX2icB2TNDPbDUhoyDibSa2pr1iaZlhImmwRmZTkIpSXaibawffRA\"\/<\/figure><\/div>\n\n
2021\u5e74QuantStudio\u7cfb\u5217\u518d\u6dfb\u65b0\u6210\u5458<\/div>\n
\u2014\u2014Applied Biosystems\u2122 QuantStudio\u2122 Absolute Q\u2122\u6570\u5b57PCR\u7cfb\u7edf\uff01<\/div>\n
\u66f4\u7b80\u4fbf\u3001\u66f4\u5feb\u901f\u3001\u66f4\u7cbe\u51c6\u5fae\u6d41\u4f53\u9635\u5217\u5f0f\u5168\u81ea\u52a8\u4e00\u4f53\u5316\u6570\u5b57PCR\u7cfb\u7edf<\/div>\n
Applied Biosystems\u2122 QuantStudio\u2122 Absolute Q\u2122\u6570\u5b57PCR\u7cfb\u7edf\u91c7\u7528\u5177\u6709\u4e13\u5229\u7684\u5fae\u6d41\u4f53\u9635\u5217\u5f0f\u82af\u7247\u6280\u672f\u3002\u76f8\u6bd4\u4e8e\u5176\u4ed6\u6570\u5b57PCR\u5e73\u53f0\uff0c\u8be5\u7cfb\u7edf\u53ef\u572820480\u4e2a\u56fa\u5b9a\u7eb3\u7c73\u7ea7\u5fae\u5b54\u4e2d\u751f\u621095%\u4ee5\u4e0a\u7684\u6709\u6548\u53cd\u5e94\u6db2\u6ef4\uff0c\u5177\u6709\u66f4\u5c11\u7684\u6b7b\u4f53\u79ef\uff0c\u4ece\u800c\u83b7\u5f97\u66f4\u7cbe\u51c6\u7684\u5b9e\u9a8c\u7ed3\u679c\u3002<\/div>\n\n
\n
\"Of3G0JtnqHusqZsa8nj3weB4nKxpHqY1e81ruxf7iaUlvxoyfgeDicicJ6WIP8uBOMBDQicBxiaYUKwADicCfb6fzprg\"\/<\/figure><\/div>\n\n
\u4f18\u52bf\u4eae\u70b9<\/div>\n
1\u3001\u7b80\u4fbf\u7684\u5de5\u4f5c\u6d41\u7a0b\uff1a\u5176\u5b9e\u9a8c\u6d41\u7a0b\u5982\u540c<\/div>\n
qPCR \u5b9e\u9a8c\uff0c\u4e00\u53f0\u673a\u5668\u5b8c\u6210\u6db2\u6ef4\u5236\u5907\u3001PCR \u6269\u589e\u3001\u8367\u5149\u4fe1\u53f7\u6536\u96c6\uff0c\u6570\u636e\u5206\u6790\u3002<\/div>\n
2\u3001\u5b9e\u9a8c\u8fd0\u884c\u5feb\u901f\uff1a\u4ece\u53cd\u5e94\u4f53\u7cfb\u5236\u5907\u5230\u7ed3\u679c\u5224\u8bfb\u53ea\u97001.5\u5c0f\u65f6\u3002<\/div>\n
3\u3001\u591a\u91cd\u68c0\u6d4b\u529f\u80fd\uff1a\u6700\u591a\u652f\u63015\u8272\u8367\u5149\u901a\u9053\u68c0\u6d4b\uff08\u5de5\u5382\u6821\u6b63\uff09\u3002<\/div>\n
4\u3001\u7ed3\u679c\u66f4\u7cbe\u51c6\uff1a\u56fa\u5b9a\u7eb3\u7c73\u7ea7\u5fae\u5b54\u4e2d\u751f\u6210<\/div>\n
95% \u4ee5\u4e0a\u7684\u6709\u6548\u53cd\u5e94\u6db2\u6ef4\uff0c\u6b7b\u4f53\u79ef<5%\uff1b\u4e14ROX\u901a\u9053\u5177\u6709\u8d28\u63a7\u529f\u80fd\uff0c\u975e\u6b63\u5e38\u6db2\u6ef4\u4e88\u4ee5\u6392\u9664\u5206\u6790\uff1bPCR\u6269\u589e\u524d\u540e\u4fe1\u53f7\u91c7\u96c6\uff0c\u81ea\u52a8\u6392\u9664\u5047\u9633\u6027\u3002<\/div>\n
5\u3001\u7075\u6d3b\u7684\u6837\u672c\u901a\u91cf\uff1a\u6bcf\u6b21\u68c0\u6d4b4\u4e2a\u30018\u4e2a\u300112\u4e2a\u621616\u4e2a\u6837\u672c\u3002<\/div>\n
6\u3001\u5168\u81ea\u52a8\u4e00\u4f53\u673a\uff1a\u5b89\u88c5\u540e\u65e0\u9700\u6821\u6b63\uff0c\u7ef4\u62a4\u6210\u672c\u4f4e\u3002<\/div>\n
\u00a0<\/div>\n
\u7b80\u4fbf\u5feb\u901f\u7684\u5de5\u4f5c\u6d41\u7a0b<\/div>\n
QuantStudio\u2122 Absolute Q\u2122\u662f\u57fa\u4e8e\u5fae\u6d41\u4f53\u9635\u5217\u5f0f\u82af\u7247\u7684\u6570\u5b57PCR\u7cfb\u7edf, \u8be5\u7cfb\u7edf\u5c06\u6db2\u6ef4\u751f\u6210\uff0c\u6db2\u6ef4\u6269\u589e\u548c\u6570\u636e\u5206\u6790\u96c6\u6210\u5230\u4e00\u4f53\u5316\u4eea\u5668\u4e2d, \u5176\u5b9e\u9a8c\u6d41\u7a0b\u5982\u540cqPCR\u5b9e\u9a8c\u4e00\u822c\uff0c\u5b9e\u73b0\u57281.5\u5c0f\u65f6\u5185\u4ece\u6837\u672c\u5230\u6570\u636e\u7ed3\u679c\u7684\u5feb\u901f\u7b80\u4fbf\u5de5\u4f5c\u6d41\u7a0b\u3002<\/div>\n\n
\n
\"Of3G0JtnqHusqZsa8nj3weB4nKxpHqY15V93jrdKM85SkzF40H6tBxmHfnTLkNnV8dQEKCb6bwkfwayNUdCdKQ\"\/<\/figure><\/div>\n\n
\u5fae\u6d41\u4f53\u9635\u5217\u5f0f\u82af\u7247(MAP16)\u677f<\/div>\n
QuantStudio\u2122 Absolute Q\u2122\u6570\u5b57PCR\u7cfb\u7edf\u91c7\u7528MAP16\u5fae\u6d41\u4f53\u9635\u5217\u5f0f\u82af\u7247\u677f\u8fdb\u884c\u6db2\u6ef4\u5236\u5907\uff0c\u8be5\u82af\u7247\u677f\u5229\u7528\u5fae\u6ce8\u5c04\u6210\u578b\u6280\u672f\uff0c\u53ef\u514b\u670d\u6db2\u6ef4\u5236\u5907\u4e0d\u5747\u4e00\u4ee5\u53ca\u6b7b\u4f53\u79ef\u8f83\u9ad8\u7684\u5e38\u89c1\u95ee\u9898\u3002\u8fd9\u79cd\u65b0\u9896\u7684\u65b9\u6cd5\u5177\u6709\u8bf8\u591a\u4f18\u52bf\uff0c\u5982\u6db2\u6ef4\u4f53\u79ef\u7cbe\u786e\u3001\u6837\u672c\u5206\u9694\u5747\u4e00\u4ee5\u53ca 95% \u4ee5\u4e0a\u7684\u4e0a\u6837\u91cf\u5206\u6790\u3002<\/div>\n\n
\n
\"Of3G0JtnqHusqZsa8nj3weB4nKxpHqY1DcedPpTFljkpH3wrNTclNJbWcxU3Wp6M2KZ835uATTsVMr8t8fZmpQ\"\/<\/figure><\/div>\n\n
\u2022 \u6bcf\u5757\u677f\u5177\u670916\u4e2a\u82af\u7247<\/div>\n
\u2022 \u6bcf\u4e2a\u82af\u7247\u53ef\u5206\u679020,000\u4e2a\u6db2\u6ef4<\/div>\n
\u2022 \u6bcf\u4e2a\u53cd\u5e94\u6b7b\u4f53\u79ef<5%<\/div>\n\n
\n
\"Of3G0JtnqHusqZsa8nj3weB4nKxpHqY1NUHgSe1cHqARKFh660yIlfv4vTkFHlJA6aW6BKSrIbq44fs5p6c7qg\"\/<\/figure><\/div>\n\n
\u6bcf\u5757MAP16\u82af\u7247\u677f\u4e0a16\u4efd\u6837\u672c\u7684\u5206\u6790\u6db2\u6ef4\u6570\u91cf\uff0c\u5171\u5bf910\u5757MAP16\u82af\u7247\u677f(160\u4efd\u6837\u672c)\u8fdb\u884c\u4e86\u8bc4\u4f30\u3002\u5fae\u5b54\u586b\u5145\u7387\u4e3a99%\u3002<\/div>\n
\u589e\u5f3a\u578b\u5149\u8def\u7cfb\u7edf<\/div>\n
QuantStudio\u2122 Absolute Q\u2122\u6570\u5b57PCR\u7cfb\u7edf\u914d\u59073\u4e2aLED\u5149\u6e90\uff0c\u6700\u591a\u652f\u63015\u4e2a\u8367\u5149\u68c0\u6d4b\u901a\u9053(\u5de5\u5382\u6821\u6b63)\uff0c\u6bcf\u4efd\u6837\u672c\u4e2d\u53ef\u8fdb\u884c\u591a\u9776\u6807\u68c0\u6d4b\uff0c\u4ece\u800c\u8282\u7701\u65f6\u95f4\u548c\u8bd5\u5242\uff0c\u83b7\u5f97\u66f4\u591a\u5b9e\u9a8c\u6570\u636e\u3002<\/div>\n
\u5176\u4e2d\uff0cROX\u901a\u9053\u4e3a\u6db2\u6ef4\u8d28\u63a7\u901a\u9053\uff0c\u53ef\u6839\u636eROX\u67d3\u6599\u7684\u8367\u5149\u4fe1\u53f7\u5f3a\u5ea6\u8bc4\u5224\u6240\u6709\u5fae\u5b54\u5185\u6db2\u6ef4\u662f\u5426\u6b63\u5e38\u751f\u6210\uff0c\u975e\u6b63\u5e38\u6db2\u6ef4\u4e88\u4ee5\u5254\u9664\u5206\u6790\uff0c\u4ece\u800c\u83b7\u5f97\u66f4\u51c6\u786e\u7ed3\u679c\u3002<\/div>\n\n
\n
\"Of3G0JtnqHusqZsa8nj3weB4nKxpHqY1c41nFT4Ocn0FXLHHDEcZvWnzZRSq9IMYqxicJzoib4Q4hdeS38UssdvA\"\/<\/figure><\/div>\n\n
\u4fbf\u6377\u6613\u7528\u7684\u5206\u6790\u8f6f\u4ef6<\/div>\n
\u8be5\u63a7\u5236\u5206\u6790\u8f6f\u4ef6\uff0c\u53ef\u8f7b\u677e\u7f16\u8f91\u677f\u9762\u5e03\u5c40\u3001\u8367\u5149\u901a\u9053\u548c\u70ed\u5faa\u73af\u7a0b\u5e8f\u7b49\u53c2\u6570\uff0c\u53ef\u4ee5\u5feb\u901f\u8fd0\u884c\u5b9e\u9a8c\u3002\u8bbe\u7f6e\u540e\uff0c\u53ef\u4ee5\u4fdd\u5b58\u548c\u91cd\u65b0\u52a0\u8f7d\u7279\u5b9a\u7a0b\u5e8f\uff0c\u7b80\u5316\u60a8\u7684\u5de5\u4f5c\u6d41\u7a0b\u3002<\/div>\n
\u68c0\u6d4b\u7ed3\u679c\u53ef\u89c6\u5316\u6216\u6280\u672f\u91cd\u590d\u6837\u672c\u8054\u5408\u5206\u6790\u65e2\u5feb\u901f\u53c8\u7b80\u5355\u3002\u7ed3\u679c\u53ef\u8f7b\u677e\u5bfc\u51fa\uff0c\u8fdb\u884c\u4e0b\u6e38\u5206\u6790\uff0c\u6216\u7f16\u8bd1\u6210\u62a5\u544a\u3002<\/div>\n
PCR\u6269\u589e\u540e\u4fe1\u53f7\u626b\u63cf\u503c\u51cf\u53bbPCR\u6269\u589e\u524d\u626b\u63cf\u503c\uff0c\u4ee5\u5254\u9664\u4efb\u4f55\u5f02\u5e38\u5982\u5f02\u7269\u3001\u53d1\u5149\u9897\u7c92\u5bfc\u81f4\u7684\u5047\u9633\u6027\u6db2\u6ef4\u3002<\/div>\n\n
\n
\"Of3G0JtnqHusqZsa8nj3weB4nKxpHqY1duOjoiayKYMFv2tzOgGibsszDhoDGWH0k2ibw753YbYHTUk8fplvxNDUg\"\/<\/figure><\/div>\n\n
<\/div>\n
\u5e94\u7528\u5e7f\u6cdb<\/div>\n
\u5bf9\u4e8e\u9700\u8981\u9ad8\u7075\u654f\u5ea6\u3001\u9ad8\u7cbe\u5ea6\u548c\u51c6\u786e\u5ea6\u7684\u68c0\u6d4b\uff0cQuantStudio\u2122 Absolute Q\u2122\u6570\u5b57PCR\u7cfb\u7edf\u662f\u4e00\u79cd\u7406\u60f3\u9009\u62e9\u3002<\/div>\n\n
\n
\"Of3G0JtnqHusqZsa8nj3weB4nKxpHqY1eTib9ibADdRhz0jQ6pp6kp2ubY5JnnkPvXSmiaOEehN5Y9VU9wLyfUFZg\"\/<\/figure><\/div>\n\n
\n
\"Of3G0JtnqHusqZsa8nj3weB4nKxpHqY1wGrvmOkw6ia3D7piaSRcTwz618ktBQd2ibRghIKlYmSjH47jzTveQP8UQ\"\/<\/figure><\/div>\n\n
\u672c\u4ea7\u54c1\u4ec5\u9650\u79d1\u7814\u4f7f\u7528\uff0c\u4e0d\u4f5c\u4e3a\u4e34\u5e8a\u8bca\u65ad\u3002<\/div>\n
<\/div>\n\n
\n
\"RmFicvQBAkgDs8t5ibGxTdCEPMR1OcqwHjYrpHxuxdeTRVtyOkgNnUW6rSxtMfMHuKhria9kgib7C0C4ic1l6MsZOMA\"\/<\/figure><\/div>\n\n
\u626b\u7801\u4e86\u89e3\u66f4\u591a\u65b0\u54c1\u4fe1\u606f<\/div>\n
\u586b\u95ee\u5377\uff0c\u9001\u597d\u793c<\/div>\n\n
\n
\"Of3G0JtnqHusqZsa8nj3weB4nKxpHqY1R20Pv2zxUsJ8YKBldgn9byOIuJyl6HpDeMG4QSgpw43napQa5Xq3zA\"\/<\/figure><\/div>\n\n
\u6269\u5c55\u9605\u8bfb<\/div>\n
\u8d5b\u9ed8\u98de\u81ea\u52a8\u5316NGS\u7cfb\u7edf\u73b0\u5df2\u4e0a\u5e02<\/a><\/div>\n
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