\n![\"RmFicvQBAkgDEsUia5jbfibMgeRrNibH1ns0NaxVdCMqRl9Bcn0geKXKbn4CByInShB9u6lDPnKmK1nhKxqqiaRaUzQ\"\/](\"https:\/\/www.geneclub.net.cn\/wp-content\/uploads\/images\/RmFicvQBAkgDEsUia5jbfibMgeRrNibH1ns0NaxVdCMqRl9Bcn0geKXKbn4CByInShB9u6lDPnKmK1nhKxqqiaRaUzQ.gif\")
<\/figure><\/div>\n\n
<\/figure><\/div>\n\n\u5173\u952e\u5b57\uff1a\u6db2\u4f53\u6d3b\u68c0ddPCR\u00a0\u00a0<\/div>\n
\u7528\u65f6\uff1a\u7ea65\u5206\u949f<\/div>\n
\u4f5c\u8005\uff1a\u5b59\u51b0\u00a0 |\u00a0\u7f16\u8005\uff1aCindy\u00a0 \u00a0|\u00a0\u6821\u5bf9\uff1a\u5341\u4e00\u90ce<\/div>\n
\u968f\u7740\u65e0\u521b\u6db2\u4f53\u6d3b\u68c0\u6280\u672f\u7684\u6210\u719f\u548c\u4f18\u5316\uff0c\u56fd\u5185\u8bb8\u591a\u673a\u6784\u6295\u5165\u4e86\u5927\u91cf\u7684\u4eba\u529b\u7269\u529b\u6765\u63a8\u5e7f\u8fd9\u4e00\u6280\u672f\u7684\u5e94\u7528\uff0c\u7279\u522b\u662f\u80bf\u7624\u68c0\u6d4b\u3001\u9884\u9632\u548c\u590d\u53d1\u8bc4\u4f30\u65b9\u9762\u3002\u8fd9\u91cc\u57fa\u56e0\u6167\u9080\u8bf7\u7f8e\u56fd\u9996\u90fd\u4e54\u6cbb\u57ce\u5927\u5b66\u533b\u5b66\u9662Lombardi\u7efc\u5408\u764c\u75c7\u7814\u7a76\u4e2d\u5fc3\u764c\u75c7\u9884\u9632\u9879\u76ee\u5206\u5b50\u751f\u7269\u5b66\u4e13\u5bb6\u5b59\u51b0\u6559\u6388\u548c\u5927\u5bb6\u5206\u4eab\u4e0d\u540c\u6280\u672f\u5728\u6db2\u4f53\u6d3b\u68c0\u4e2d\u5e94\u7528\u7684\u573a\u666f\u53ca\u6ce8\u610f\u4e8b\u9879\uff0c\u4f9b\u5927\u5bb6\u53c2\u8003\uff0c\u6b22\u8fce\u7559\u8a00\u8ba8\u8bba\u3002<\/div>\n
\u00a0<\/div>\n
\u5b59\u51b0<\/div>\n\n
\u7f8e\u56fd\u9996\u90fd\u4e54\u6cbb\u57ce\u5927\u5b66\u533b\u5b66\u9662Lombardi\u7efc\u5408\u764c\u75c7\u7814\u7a76\u4e2d\u5fc3\u764c\u75c7\u9884\u9632\u9879\u76ee\u5206\u5b50\u751f\u7269\u5b66\u4e13\u5bb6\u3001\u5c71\u4e1c\u5927\u5b66\u751f\u6b96\u533b\u9662\u5ba2\u5ea7\u6559\u6388<\/div>\n
\u00a0<\/div>\n
1\u3001\u4e0d\u540c\u6280\u672f\u5728\u65e0\u521b\u6db2\u4f53\u6d3b\u68c0\u4e2d\u5e94\u7528<\/div>\n
\u4e8c\u4ee3\u6d4b\u5e8f\uff08NGS\uff09\u5728\u56fd\u5185\u5df2\u88ab\u5e7f\u6cdb\u5e94\u7528\u4e8e\u79d1\u7814\u7684\u5404\u4e2a\u9886\u57df\u548c\u4ea7\u524d\u8bca\u65ad\uff0c\u6db2\u4f53\u6d3b\u68c0\u4e5f\u4e0d\u4f8b\u5916\u3002\u8d28\u8c31\uff08MassArray\uff09\u4e5f\u53ef\u4ee5\u7528\u4e8e\u8840\u6db2\u65e0\u521b\u68c0\u6d4b\uff0c\u5176\u7075\u654f\u5ea6\u53ef\u8fbe\u5230\u5343\u5206\u4e4b\u4e00\u3002\u5fae\u6ef4\u6570\u5b57\u5316PCR\uff0c\u4f5c\u4e3a\u7b2c\u4e09\u4ee3DNA\u653e\u5927\u6280\u672f\uff0c\u867d\u88ab\u5927\u5bb6\u516c\u8ba4\u4e3a\u662f\u76ee\u524d\u6700\u654f\u611f\u7684\u68c0\u6d4b\u65b9\u6cd5\uff0c\u4f46\u5b9e\u9645\u5e94\u7528\u4e2d\u5176\u7075\u654f\u5ea6\u53c8\u548c\u8bb8\u591a\u56e0\u7d20\u606f\u606f\u76f8\u5173\u3002<\/div>\n
\u4e0a\u9762\u63d0\u5230\u7684\u4e09\u4e2a\u6280\u672f\uff0c\u5355\u7eaf\u6839\u636e\u5176\u68c0\u6d4b\u7247\u6bb5\u7684\u5927\u5c0f\u5c31\u51b3\u5b9a\u4e86\u662f\u5426\u9002\u7528\u4e8e\u6db2\u4f53\u6d3b\u68c0\u3002\u76ee\u524d\u5728\u4e34\u5e8a\u4e0a\u836f\u68c0\u5c40\uff08CFDA\uff09\u5df2\u7ecf\u6279\u51c6\u4f7f\u7528\u7684ARMS\u5b9e\u65f6\u5b9a\u91cfPCR\uff08ARMS-qPCR\uff09\u6cd5\uff0c\u5176\u7075\u654f\u5ea6\u4e5f\u5728\u5343\u5206\u4e4b\u4e00\uff0c\u4f46\u5176\u653e\u5927\u7247\u6bb5\u7684\u957f\u5ea6\u51b3\u5b9a\u4e86\u662f\u5426\u53ef\u7528\u4e8e\u8840\u6db2\u6807\u672c\u7684\u68c0\u6d4b\uff08\u89e3\u91ca\u89c1\u4e0b\uff09\u3002MicroArray\u5df2\u88ab\u5e7f\u6cdb\u5e94\u7528\u4e8e\u7ec6\u80de\u6216\u7ec4\u7ec7\u7684\u57fa\u56e0\u8868\u8fbe\u8c31\uff08profiling\uff09\u7684\u7814\u7a76\u3002NGS\u4e5f\u7528\u6765\u68c0\u6d4bmRNA\u5e76\u53ef\u6839\u636e\u6d4b\u5e8f\u7684\u7ed3\u679c\u5206\u6790\u57fa\u56e0\u8868\u8fbe\u7684\u60c5\u51b5\uff0c\u800c\u8868\u8fbe\u8c31\u7684\u7814\u7a76\u5219\u57fa\u4e8e\u68c0\u6d4b\u7684\u6df1\u5ea6\uff08dynamic range\uff09\u3002NGS\u4e3b\u8981\u68c0\u6d4b\u5728\u8868\u8fbe\u7684\u57fa\u56e0\u5e8f\u5217\u4e0a\u662f\u5426\u6709\u6539\u53d8\uff0c\u4f46\u82e5\u7528\u6765\u505a\u57fa\u56e0\u8868\u8fbe\u8c31\u8d39\u7528\u4f1a\u76f8\u5bf9\u8f83\u9ad8\u3002<\/div>\n
\u00a0<\/div>\n
2\u3001ddPCR\u548cNGS\u76f8\u8f85\u76f8\u6210<\/div>\n
\u53ef\u4ee5\u628addPCR\u548cNGS\u770b\u6210\u662f\u70b9\u548c\u7ebf\u7684\u5173\u7cfb\u3002\u4e24\u8005\u5728\u5e94\u7528\u4e0a\u5e94\u8be5\u662f\u76f8\u8f85\u76f8\u6210\u7684\u3002NGS\u662f\u7528\u6765\u68c0\u6d4b\u4e00\u6761\u7ebf\u4e0a\u7684\u6bcf\u4e00\u70b9\u662f\u5426\u53d1\u751f\u4e86\u6539\u53d8\uff0c\u800cddPCR\u5219\u662f\u4e13\u6ce8\u4e8e\u68c0\u6d4b\u591a\u6761\u7ebf\u4e0a\u7684\u67d0\u4e00\u70b9\u662f\u5426\u53d1\u751f\u4e86\u6539\u53d8\u3002\u867d\u7136\u6709\u4eba\u8ba4\u4e3aNGS\u7684\u6d4b\u5e8f\u7ed3\u679c\u4e5f\u53ef\u4ee5\u7528\u6765\u5206\u6790\u67d0\u4e00\u70b9\u7684\u6539\u53d8\uff0c\u4f46\u8fd9\u5b8c\u5168\u53d6\u51b3\u4e8e\u6d4b\u5e8f\u7684\u6df1\u5ea6\u3002<\/div>\n
\u5f53\u521a\u62ff\u5230\u4e00\u4e2a\u75c5\u4eba\u7684\u80bf\u7624\u6837\u672c\u65f6\uff0c\u6700\u597d\u662f\u80fd\u505a\u4e00\u4e0bNGS\uff0c\u5e2e\u52a9\u627e\u5230\u8fd9\u4e2a\u75c5\u4eba\u7684\u6837\u672c\u4e2d\u6709\u54ea\u4e9b\u53d8\u5f02\uff0c\u800c\u4e0d\u5efa\u8bae\u4ec5\u4ec5\u6839\u636e\u4ee5\u5f80\u6587\u732e\u7684\u62a5\u9053\u6765\u63a8\u6d4b\u67d0\u4e9b\u57fa\u56e0\u53ef\u80fd\u5728\u8fd9\u4e2a\u75c5\u4eba\u53d1\u751f\u7684\u7a81\u53d8\u3002\u63a5\u4e0b\u6765\u8981\u76d1\u6d4b\u75c5\u4eba\u7684\u624b\u672f\u6216\u653e\u5316\u7597\u7684\u7597\u6548\u65f6\uff0c\u56e0\u4e3a\u6709\u4e86\u524d\u9762NGS\u6d4b\u5e8f\u7684\u7ed3\u679c\uff0c\u8003\u8651\u5230\u66f4\u7075\u654f\u3001\u66f4\u5feb\u901f\u548c\u66f4\u5ec9\u4ef7\uff0cddPCR\u5219\u662f\u5f88\u597d\u7684\u9009\u62e9\u4e4b\u4e00\uff08\u5982\u4e0b\u56fe\uff09\u3002\u4ece\u6210\u672c\u548c\u6280\u672f\u4e0a\uff0c\u7528ddPCR\u53ef\u76f8\u5bf9\u5bb9\u6613\u8fbe\u52302\u4e07x\u7684\u68c0\u6d4b\u6df1\u5ea6\uff08NGS\u7684\u68c0\u6d4b\u6df1\u5ea6\u4e00\u822c\u4e0d\u4f1a\u8d85\u8fc73\u5343x\uff09\u3002<\/div>\n\n
\n![\"RmFicvQBAkgDEsUia5jbfibMgeRrNibH1ns0hgyomxFbibXD1LCYmkibguU884wTbiajsVw1hd08Kgbd09eYyliaE7cCJA\"\/](\"https:\/\/www.geneclub.net.cn\/wp-content\/uploads\/images\/RmFicvQBAkgDEsUia5jbfibMgeRrNibH1ns0hgyomxFbibXD1LCYmkibguU884wTbiajsVw1hd08Kgbd09eYyliaE7cCJA.png\")
<\/figure><\/div>\n\n
<\/figure><\/div>\n\n\u56fe\uff1actDNA\u68c0\u6d4b\u6280\u672f\u6bd4\u8f83<\/div>\n
3\u3001ctDNA\u7684\u68c0\u6d4b\u4e2d\u7684\u6d53\u5ea6\u548c\u6a21\u677f\u957f\u5ea6\u6307\u6807<\/div>\n
\u6d4b\u5b9a\u8840\u4e2d\u6e38\u79bb\u7684\u5faa\u73af\u80bf\u7624DNA\uff08ctDNA\uff09\u5bf9\u4e8e\u80bf\u7624\u7684\u65e9\u671f\u8bca\u65ad\u3001\u836f\u7269\u9009\u62e9\u548c\u5b9e\u65f6\u7597\u6548\u548c\u8010\u836f\u76d1\u6d4b\u6709\u7740\u5e7f\u6cdb\u7684\u5b9e\u7528\u4ef7\u503c\u548c\u53d1\u5c55\u524d\u666f\u3002<\/div>\n
\u5bf9\u68c0\u6d4b\u7684\u624b\u6bb5\u6216\u6280\u672f\u5c24\u5176\u662f\u7075\u654f\u5ea6\u6709\u5f88\u9ad8\u7684\u8981\u6c42\uff0c\u4e3b\u8981\u662f\u56e0\u4e3a\u8840\u4e2d\u53ef\u63d0\u53d6\u5230\u7684\u6e38\u79bbDNA\u4e0d\u591a\uff0c\u4e14\u80bf\u7624DNA\u6240\u5360\u7684\u6bd4\u4f8b\u5f88\u4f4e\u3002\u6839\u636e\u4e00\u4e2a\u5b8c\u6574\u7684\u57fa\u56e0\u7ec4DNA\u5927\u7ea6\u76f8\u5f53\u4e8e3pg\uff0c\u4e00\u822c\u8ba4\u4e3a\u9700\u8981\u4e09\u500d\u7684DNA\u91cf\u624d\u80fd\u786e\u5b9a\u68c0\u6d4b\u7684\u7cbe\u5ea6\uff0c\u6240\u4ee5\u516c\u8ba4\u4e3a\u9700\u898110ng\u6765\u505a\u5343\u5206\u4e4b\u4e00\u7684\u68c0\u6d4b\u3002\u8fd9\u662f\u57fa\u4e8e\u4ece\u7ec6\u80de\u6216\u7ec4\u7ec7\u4e2d\u63d0\u53d6\u51fa\u5b8c\u6574\u7684\u57fa\u56e0\u7ec4DNA\u63a8\u7b97\u7684\u3002<\/div>\n
\u7136\u800c\uff0c\u5f53\u68c0\u6d4b\u7684\u6807\u672c\u662f\u8840\u6db2\u4e2d\u7684\u6e38\u79bbDNA\uff0c\u68c0\u6d4b\u6a21\u677f\u7684\u957f\u5ea6\u4e5f\u662f\u4e00\u4e2a\u5f88\u91cd\u8981\u7684\u6307\u6807\u3002\u6bd4\u5982\uff0c\u68c0\u6d4b\u7684DNA\u7247\u6bb5\u82e5\u5728200bp\u4ee5\u4e0a\uff0c\u7528\u5728\u8840\u6db2\u6e38\u79bbDNA\u7684\u68c0\u6d4b\uff0c\u5373\u4f7f\u6709\u5f88\u591a\u68c0\u6d4b\u7684\u9776\u5411\u76ee\u6807\u5b58\u5728\uff0c\u4f46\u68c0\u6d4b\u5230\u7684\u53ef\u80fd\u6027\u4e0d\u5927\u3002\u8fd9\u4e3b\u8981\u662f\u56e0\u4e3a\u8840\u6db2\u4e2d\u7684\u6e38\u79bbDNA\u5927\u90fd\u662f\u5728\u7ec6\u80de\u51cb\u4ea1\uff08apoptosis\uff09\u8fc7\u7a0b\u4e2d\u88ab\u964d\u89e3\u6210165bp\u6216\u66f4\u5c0f\u7684\u7247\u6bb5\uff08\u5982\u4e0b\u56fe\uff09\u3002\u867d\u7136\u76ee\u524d\u5c1a\u4e0d\u6e05\u695aDNA\u5728apoptosis\u7684\u964d\u89e3\u8fc7\u7a0b\u4e2d\u7684\u964d\u89e3\u4f4d\u70b9\u662f\u5426\u6709\u7279\u5f02\u6027\uff0c\u4f46\u51ed\u5176\u968f\u673a\u964d\u89e3\u540e\u7247\u6bb5\u7684\u5927\u5c0f\uff0c\u7528\u4e8e\u68c0\u6d4b\u7684DNA\u7247\u6bb5\u6700\u597d\u5e94\u63a7\u5236\u5728150bp\u4ee5\u4e0b\u3002\u53ef\u4ee5\u7b80\u5355\u7684\u7b97\u4e00\u4e0b\uff0c\u82e5\u68c0\u6d4b\u7684DNA\u7247\u6bb5\u662f55bp\uff0c\u76f8\u5bf9\u4e8e\u5b8c\u6574\u7684\u57fa\u56e0\u7ec4DNA\uff0c\u4ece\u8840\u4e2d\u6e38\u79bbDNA\u68c0\u6d4b\u5230\u7684\u76ee\u6807\u57fa\u56e0\u7684\u51e0\u7387\u964d\u4f4e\u4e861\/3\u3002\u5f53\u68c0\u6d4b\u7684DNA\u7247\u6bb5\u589e\u52a0\u5230110bp\uff0c\u68c0\u6d4b\u5230\u7684\u51e0\u7387\u53c8\u964d\u4e861\/3\u3002\u6240\u4ee5\u79d1\u7814\u4eba\u5458\u9996\u5148\u9700\u8981\u641e\u6e05\u695a\u6240\u9009\u7528\u7684\u68c0\u6d4b\u65b9\u6cd5\uff08\u8bd5\u5242\u76d2\uff09\u7684\u68c0\u6d4b\u7247\u6bb5\u7684\u5927\u5c0f\uff0c\u786e\u5b9a\u662f\u5426\u9002\u7528\u4e8e\u8840\u4e2d\u6e38\u79bbDNA\u7684\u68c0\u6d4b\uff0c\u8fd8\u9700\u8ba1\u7b97\u51fa\u6bcf\u4e2a\u68c0\u6d4b\u6240\u9700\u7528\u7684\u6837\u672c\u91cf\u3002<\/div>\n\n
\n![\"RmFicvQBAkgDEsUia5jbfibMgeRrNibH1ns06LdV8AvsicoeBXvPlPM14RGeFZ5jcbdthLmvqHibrZWJqvDlaAlDpksQ\"\/](\"https:\/\/www.geneclub.net.cn\/wp-content\/uploads\/images\/RmFicvQBAkgDEsUia5jbfibMgeRrNibH1ns06LdV8AvsicoeBXvPlPM14RGeFZ5jcbdthLmvqHibrZWJqvDlaAlDpksQ.png\")
<\/figure><\/div>\n\n
<\/figure><\/div>\n\n\u56fe\uff1a\u6838\u5c0f\u4f53\u7684\u5f62\u6210\u793a\u610f\u56fe<\/div>\n
4\u3001\u6839\u636e\u68af\u5ea6PCR\u7ed3\u679c\u91c7\u7528\u9700\u8981\u68c0\u6d4b\u7684\u6807\u672c\u7c7b\u578b<\/div>\n
\u5f53\u68c0\u6d4b\u53cd\u5e94\u4e2d\u52a0\u5165\u7684\u6837\u672c\u91cf\u8fc7\u591a\uff0c\u5219\u4f1a\u51fa\u73b0\u53e6\u4e00\u4e2a\u8ba9\u4eba\u5934\u75db\u7684\u95ee\u9898\uff0c\u90a3\u5c31\u662f\u7279\u5f02\u6027\u7684\u95ee\u9898\u3002<\/div>\n
\u6709\u7ecf\u9a8c\u7684\u4eba\u4e00\u63d0\u5c31\u77e5\u9053\uff0c\u4e8b\u5148\u7528\u4ece\u7ec6\u80de\u63d0\u53d6\u7684\u57fa\u56e0\u7ec4DNA\u5efa\u7acb\u8d77\u7684\u65b9\u6cd5\u548c\u6761\u4ef6\uff0c\u5f53\u6539\u7528\u4ece\u798f\u5c14\u9a6c\u6797\u56fa\u5b9a\u7684\u8721\u5757\u7ec4\u7ec7\u6216\u4ece\u8840\u6d46\/\u8840\u6e05\u4e2d\u63d0\u53d6\u7684DNA\u65f6\uff0cPCR\u653e\u5927\u5f80\u5f80\u4f1a\u6216\u591a\u6216\u5c11\u51fa\u73b0\u4e00\u4e9b\u975e\u7279\u5f02\u6027\u3002\u5f53\u5382\u5bb6\u63a8\u9500\u8bd5\u5242\u76d2\u65f6\u63d0\u5230\u66fe\u5c06\u9633\u6027\u6807\u672c\u7a00\u91ca1\u5343\u751a\u81f31\u4e07\u500d\u800c\u4ecd\u80fd\u68c0\u6d4b\u7684\u51fa\u65f6\uff0c\u5e94\u8be5\u660e\u786e\u4e00\u4e0b\u662f\u7528\u4ec0\u4e48\u6837\u7684\u6807\u672c\u6765\u505a\u7a00\u91ca\u3002<\/div>\n
\u5f53\u4f60\u81ea\u5df1\u5728\u4f18\u5316\u53cd\u5e94\u6761\u4ef6\u65f6\uff0c\u4e0d\u59a8\u6839\u636e\u68af\u5ea6PCR\u7684\u7ed3\u679c\uff0c\u627e\u5230\u6700\u9ad8\u7684\uff08stringent\uff09\u9000\u706b\uff08annealing\uff09 \u6e29\u5ea6\uff0c\u5e76\u76f4\u63a5\u91c7\u7528\u9700\u8981\u68c0\u6d4b\u7684\u6807\u672c\u7c7b\u578b\u6765\u8fdb\u884c\u4f18\u5316\u3002\u53e6\u5916\uff0c\u5c31\u662f\u8003\u8651\u57283\u52305\u4e2aPCR\u5faa\u73af\u653e\u5927\u540e\uff0c\u964d\u4f4e\u89e3\u94fe\uff08denature\uff09\u7684\u6e29\u5ea6\u81f3\u53ea\u80fd\u591f\u4f7f\u5f97\u524d\u51e0\u8f6e\u653e\u5927\u7684\u5c0f\u7247\u6bb5\u8db3\u4ee5\u89e3\u94fe\u5c31\u53ef\u3002<\/div>\n
\u00a0<\/div>\n
\u53d6\u8840\u6837\u505a\u6db2\u4f53\u6d3b\u68c0\u65f6\u8fd8\u8981\u4fdd\u8bc1\u6837\u672c\u65b0\u9c9c\u3002\u57fa\u672c\u539f\u5219\u662f\u5ba4\u6e29\u4e0b\u62bd\u8840\u540e4\u5c0f\u65f6\u5185\u5c06\u8840\u6d46\u5206\u79bb\u5b8c\u6bd5\u4f4e\u6e29\u51bb\u5b58\uff0c\u6216\u7528\u7279\u5236\u7684\u62bd\u8840\u7ba1\u5982Qiagen\u516c\u53f8\u7684PAXgene\u53d6\u6837\u7ba1\uff0c\u5176\u76ee\u7684\u5c31\u662f\u907f\u514d\u7531\u8840\u4e2d\u767d\u7ec6\u80de\u957f\u65f6\u95f4\u5b58\u653e\u540e\u91ca\u653e\u51fa\u7684\u57fa\u56e0\u7ec4DNA\u6c61\u67d3\u8840\u6d46\u4e2d\u7684\u6e38\u79bbDNA\uff0c\u9020\u6210\u8fdb\u4e00\u6b65\u964d\u4f4e\u80bf\u7624\u57fa\u56e0\u88ab\u68c0\u6d4b\u5230\u7684\u51e0\u7387\u3002<\/div>\n
\u8fd8\u6709\u4e00\u70b9\u5c31\u662f\u5bf9\u4e8e\u63d0\u53d6\u8840\u4e2d\u6e38\u79bbDNA\u8bd5\u5242\u76d2\u7684\u9009\u62e9\u3002\u6709\u7684\u516c\u53f8\u7684\u8bd5\u5242\u76d2\u66f4\u503e\u5411\u4e8e\u4ece\u8840\u6d46\/\u8840\u6e05\u4e2d\u63d0\u53d6\u7eaf\u5316\u8f83\u5927\u7247\u6bb5\u7684DNA\uff0c\u56e0\u6b64\u5e76\u4e0d\u9002\u7528\u4e8e\u8840\u6db2\u6db2\u4f53\u6d3b\u68c0\u7684\u8981\u6c42\u3002<\/div>\n
\u00a0<\/div>\n
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\u00a0<\/div>\n
\u5f53\u9700\u8981\u76d1\u6d4b\u6cbb\u7597\u7684\u7597\u6548\u6216\u75c5\u60c5\u7684\u8fdb\u5c55\u65f6\uff0c\u68c0\u6d4b\u7684\u539f\u5219\u662f\u4e0d\u4ec5\u9700\u8981\u8f83\u9ad8\u7684\u7075\u654f\u5ea6\u8fd8\u9700\u8981\u51c6\u786e\u7684\u5b9a\u91cf\u3002\u5faa\u73af\u4e2d\u6e38\u79bb\u7684\u80bf\u7624DNA\uff0c\u56e0\u4e3a\u5176\u534a\u8870\u671f\u53ea\u67091\u4e2a\u534a\u5c0f\u65f6\u5de6\u53f3\uff0c\u800c\u4e14\u5176\u8fdb\u5165\u8840\u5faa\u73af\u662f\u4e00\u4e2a\u6301\u7eed\u7684\u8fc7\u7a0b\uff0c\u5176\u5728\u8840\u4e2d\u7684\u6c34\u5e73\u53ef\u4ee5\u5f88\u51c6\u786e\u7684\u7528\u6765\u53cd\u6620\u75be\u75c5\u7684\u8fdb\u5c55\u60c5\u51b5\u3002ddPCR\u6280\u672f\uff0c\u56e0\u4e3a\u662f\u76f4\u63a5\u8ba1\u6570\u6765\u7edd\u5bf9\u5b9a\u91cf\u800c\u4e0d\u9700\u8981\u5185\u63a7\u6807\u51c6\u54c1\u505a\u5b9a\u91cf\u53c2\u8003\uff0c\u5728\u52a0\u4e4b\u8f83\u5bb9\u6613\u7684\u5224\u8bfb\uff0c\u5728\u6db2\u4f53\u6d3b\u68c0\u5e94\u7528\u4e2d\u8f83ARMS-qPCR\u6cd5\u663e\u793a\u51fa\u660e\u663e\u7684\u4f18\u52bf\u3002\u53c8\u56e0\u4e3a\u5176\u7075\u654f\u5ea6\u9ad8\u800c\u68c0\u6d4b\u8d39\u7528\u4f4e\u518d\u52a0\u4e4b\u7ed3\u679c\u62a5\u544a\u5468\u671f\u77ed\uff0c\u5728\u8fd9\u4e00\u70b9\u4e0a\u6bd4NGS\u66f4\u6709\u5b9e\u7528\u4ef7\u503c\u3002<\/div>\n
\u00a0<\/div>\n
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